Itk (NM_001281968) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Emt; Tcsk; Tsk
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228486 representing NM_001281968 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACAACTTCATCCTCCTGGAAGAACAGCTGATCAAGAAGTCCCAACAGAAGAGAAGGACTTCTCCCT CGAATTTTAAAGTTCGTTTCTTTGTCTTAACGAAAGCCAGCCTGGCCTACTTTGAGGACCGCCACGGGAA GCGCACGTTGAAGGGCTCCATTGAACTCTCCAGAATCAAGTGTGTGGAGATTGTCAAGAGTGACATTAGC ATCCCGTGCCACTATAAATACCCTTTTCAGGTCGTGCATGACAACTATCTCCTGTATGTGTTTGCTCCAG ACTGTGAGAGTCGGCAGCGCTGGGTGCTGACCCTTAAAGAAGAAACGAGGAATAACAACAGCCTGGTATC CAAGTATCACCCTAATTTCTGGATGGATGGGCGGTGGAGGTGCTGCTCCCAGCTGGAGAAGCCTGCTGTA GGCTGTGCTCCCTACGACCCATCCAAGAATGCTTCAAAGAAGCCTCTTCCTCCTACTCCTGAAGACAACA GGCGGTCATTTCAGGAACCTGAAGAAACCCTGGTCATTGCCTTGTACGACTACCAAACCAACGACCCTCA GGAGCTCGCACTGCGGTGTGATGAAGAGTACTACCTGCTGGACAGCTCCGAGATCCACTGGTGGAGGGTT CAAGACAAAAATGGGCATGAAGGATATGCACCAAGCAGTTACCTGGTAGAAAAATCTCCAAATAACCTTG AAACCTATGAGTGGTACAATAAAAGCATCAGCCGCGACAAAGCTGAAAAACTTCTTTTGGACACAGGTAA AGAAGGAGCTTTCATGGTCCGAGATTCCAGGACACCCGGGACATACACAGTCTCTGTTTTCACCAAGGCC ATCATAAGTGAGAACCCCTGTATAAAACATTATCACATCAAAGAAACAAATGACAGCCCCAAGCGCTACT ACGTGGCTGAGAAGTATGTGTTTGACTCCATCCCTCTCCTCATCCAGTATCACCAGTACAATGGAGGAGG TTTGGTCACTCGACTCCGCTATCCAGTTTGCTCCTGGAGACAAAAAGCCCCTGTCACAGCAGGGCTAAGA TATGGGAAGTGGGTGATCCAACCCTCAGAGCTAACGTTCGTGCAGGAGATTGGCAGCGGGCAGTTTGGGC TGGTGCATCTCGGCTACTGGCTCAACAAGGACAAGGTGGCCATCAAGACCATTCAGGAAGGGGCGATGTC AGAAGAAGACTTTATCGAGGAGGCGGAAGTCATGATGAAACTCTCTCACCCCAAACTGGTGCAGCTCTAT GGGGTGTGCCTGGAGCAAGCCCCCATCTGCCTGGTGTTTGAGTTCATGGAGCACGGCTGCCTGTCGGATT ACCTTCGAAGTCAGCGGGGTCTCTTTGCTGCGGAGACCCTGCTGGGCATGTGCCTGGATGTGTGTGAGGG CATGGCCTACCTGGAAAAAGCTTGTGTCATCCACAGAGACCTGGCAGCCAGAAACTGTTTGGTGGGAGAA AACCAGGTCATCAAGGTGTCCGACTTTGGGATGACAAGATTTGTCCTTGATGATCAATATACCAGCTCCA CGGGCACCAAATTCCCAGTGAAGTGGGCATCCCCAGAGGTGTTCTCCTTTAGTCGCTATAGCAGCAAGTC AGATGTGTGGTCGTTTGGTGTACTGATGTGGGAAGTCTTCAGTGAAGGCAAAATCCCATACGAAAACCGA AGCAATTCCGAGGTCGTGGAAGATATCAGCACTGGCTTTCGGTTATACAAGCCCCGCCTGGCCTCCTGTC ATGTCTACCAGATCATGAATCATTGCTGGAAAGAGAAACCAGAAGACCGGCCACCCTTCTCCCAGCTGTT AAGCCAGCTGGCTGAAATCGCAGAAGCTGGGCTTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001281968
Insert Size	1857 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001281968.1, NP_001268897.1
RefSeq Size	4271 bp
RefSeq ORF	1857 bp
Locus ID	16428
UniProt ID	Q03526
Gene Summary	Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Phosphorylates TBX21 at 'Tyr-525' and mediates its interaction with GATA3 (PubMed:15662016).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) uses two alternate in-frame splice sites in the central coding region, compared to variant 1. The encoded isoform (5) is shorter, compared to isoform 1.

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