Ache (NM_001290010) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228471 representing NM_001290010 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCCTCCCTGGTATCCCCTGCATACACCTTCCCTGGCTTTTCCACTCCTCTTCCTCCTCCTCTCCC TCCTGGGAGGAGGGGCAAGGGCTGAGGGCCGGGAAGACCCGCAGCTGCTGGTGAGGGTTCGAGGGGGCCA GCTGAGGGGCATCCGCCTGAAGGCCCCTGGAGGCCCAGTCTCAGCTTTTCTGGGCATCCCCTTTGCAGAG CCACCTGTGGGCTCACGTAGATTTATGCCACCAGAGCCCAAGCGGCCCTGGTCAGGAGTGTTGGATGCTA CCACCTTCCAAAATGTCTGCTACCAGTACGTGGACACCCTGTACCCTGGGTTTGAGGGTACTGAGATGTG GAACCCCAACCGAGAGTTGAGTGAAGACTGCCTGTATCTTAATGTGTGGACACCATACCCCAGACCTGCT TCTCCCACACCTGTCCTCATCTGGATCTATGGGGGTGGTTTCTACAGCGGAGCGGCCTCCTTGGATGTGT ATGACGGCCGTTTCCTGGCCCAGGTTGAGGGAGCTGTGTTGGTATCTATGAACTACCGAGTGGGAACCTT TGGCTTCTTGGCCCTACCAGGAAGCAGAGAAGCCCCTGGCAATGTAGGTCTGCTGGATCAACGGCTTGCC TTGCAATGGGTGCAAGAAAATATTGCAGCCTTTGGGGGCGACCCGATGTCAGTGACTCTGTTTGGGGAGA GTGCGGGTGCAGCCTCCGTGGGCATGCACATACTGTCCCTGCCCAGCAGGAGCCTCTTCCACAGGGCTGT CCTCCAGAGTGGCACACCCAATGGGCCCTGGGCCACTGTGAGTGCTGGAGAGGCCAGGCGCAGGGCCACA CTGCTGGCCCGCCTTGTGGGCTGTCCCCCAGGTGGCGCTGGTGGCAATGACACCGAGCTGATAGCCTGCT TGAGGACAAGGCCCGCTCAGGACCTGGTGGACCACGAGTGGCACGTCCTGCCTCAAGAAAGTATCTTCCG ATTTTCCTTCGTGCCTGTGGTAGACGGGGACTTCCTCAGTGACACACCGGAGGCTCTCATCAATACTGGA GATTTTCAAGACCTGCAGGTGCTGGTGGGTGTGGTGAAGGACGAGGGCTCCTACTTTCTGGTTTACGGGG TCCCAGGCTTCAGCAAAGACAATGAATCTCTCATCAGCCGGGCCCAGTTCCTGGCTGGGGTGCGGATCGG TGTACCCCAAGCAAGTGACCTGGCAGCCGAGGCTGTGGTCCTGCATTACACAGACTGGCTGCACCCTGAG GACCCTACTCACCTGAGAGATGCCATGAGTGCAGTGGTAGGCGACCACAACGTTGTGTGCCCTGTGGCCC AGCTGGCTGGGCGACTGGCTGCCCAAGGGGCCCGGGTCTATGCCTACATCTTTGAACACCGTGCCTCCAC ACTGACTTGGCCCCTCTGGATGGGGGTGCCCCATGGCTATGAAATCGAGTTCATCTTTGGGCTCCCCCTG GATCCCTCGCTGAACTACACCACGGAGGAGAGGATCTTTGCTCAGCGACTTATGAAATACTGGACCAATT TTGCCCGCACAGGGGACCCCAATGACCCTCGAGACTCCAAATCTCCACAGTGGCCACCGTACACCACTGC CGCGCAGCAATATGTGAGCCTGAACCTGAAGCCCTTAGAGGTGCGGCGGGGACTGCGCGCCCAGACCTGC GCCTTCTGGAATCGCTTTCTCCCCAAATTGCTCAGCGCCACCGATACTCTGGACGAGGCGGAGCGCCAGT GGAAGGCCGAGTTCCACCGCTGGAGCTCCTACATGGTGCACTGGAAGAACCAGTTCGACCACTATAGCAA GCAGGAGCGCTGCTCAGACCTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290010
Insert Size	1845 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290010.1, NP_001276939.1
RefSeq Size	2227 bp
RefSeq ORF	1845 bp
Locus ID	11423
UniProt ID	P21836
Gene Summary	Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.

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