Arntl (NM_001243048) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Arnt3; bHLHe5; Bmal1; BMAL1b; bmal1b'; MOP3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228467 representing NM_001243048 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACCAGAGAATGGACATTTCCTCAACCATCAGCGACTTCATGTCTCCGGGCCCCACCGACCTAC TCTCCGGTTCCCTGGGCACCAGTGGTGTGGACTGCAATCGCAAGAGGAAAGGCAGTGCCACTGACTACCA GTTAGAATATGCAGAACACCAAGGAAGGATCAAGAATGCAAGGGAGGCCCACAGTCAGATTGAAAAGAGG CGTCGGGACAAAATGAACAGTTTCATTGATGAATTGGCTTCTTTGGTACCAACATGCAATGCAATGTCCA GGAAGTTAGATAAACTCACCGTGCTAAGGATGGCTGTTCAGCACATGAAAACTTTGAGAGGTGCCACCAA CCCATACACAGAAGCAAACTACAAGCCAACATTTCTATCAGATGACGAACTGAAACACCTAATTCTCAGG GCAGCAGATGGATTTTTGTTTGTCGTAGGATGTGACCGAGGGAAGATCCTCTTTGTCTCCGAGTCTGTCT TCAAGATCCTCAATTATAGCCAGAATGACCTTATTGGCCAGAGCTTGTTTGACTACCTGCATCCAAAAGA TATTGCCAAAGTTAAGGAACAGCTATCTTCCTCGGACACTGCGCCCCGGGAGCGACTCATTGATGCCAAG ACTGGACTTCCGGTTAAAACGGATATAACCCCTGGGCCCTCCCGGCTATGCTCTGGAGCCCGCCGCTCTT TCTTCTGTAGAATGAAGTGCAACAGGCCTTCAGTAAAGGTGGAAGATAAGGACTTCGCCTCTACCTGTTC AAAGAAAAAAGCAGATCGAAAAAGCTTCTGCACAATCCACAGCACAGGCTATTTGAAAAGCTGGCCACCC ACGAAGATGGGGCTGGACGAAGACAATGAGCCAGACAACGAGGGCTGCAACCTCAGCTGCCTCGTTGCAA TCGGGCGCCTGCACTCGCACATGGTTCCACAACCAGCGAACGGGGAAATACGGGTGAAATCTATGGAGTA CGTTTCTCGACACGCAATAGATGGGAAATTTGTTTTTGTAGATCAGAGGGCGACAGCTATTTTGGCGTAT CTACCACAGGAACTTCTAGGTACATCATGTTATGAGTATTTTCATCAAGACGACATAGGACACCTCGCAG AATGTCACAGGCAAGTTTTACAGACAAGAGAAAAGATCACAACTAATTGCTATAAGTTTAAGATCAAAGA TGGTTCTTTTATCACGCTACGAAGTCGATGGTTCAGTTTCATGAACCCGTGGACCAAGGAAGTTGAATAC ATTGTCTCAACCAACACTGTTGTTTTAGCCAATGTCCTGGAAGGCGGGGACCCAACCTTCCCGCAGCTAA CAGCACCCCCCCACAGCATGGACAGCATGCTGCCCTCTGGAGAAGGTGGCCCAAAGAGGACTCATCCCAC TGTCCCAGGCATTCCAGGGGGAACCAGAGCCGGAGCAGGAAAAATAGGTCGAATGATCGCGGAGGAAATC ATGGAAATCCACAGGATAAGAGGGTCATCGCCTTCCAGCTGTGGCTCCAGCCCGCTGAACATCACAAGTA CGCCTCCCCCTGATGCCTCTTCTCCAGGAGGCAAGAAGATTCTAAATGGAGGGACTCCAGACATTCCTTC CACTGGACTATTACCAGGGCAGGCTCAGGAGACCCCAGGGTATCCCTATTCTGATAGTTCTTCTATTCTT GGTGAGAACCCCCACATAGGCATCGATATGATAGATAACGACCAAGGATCAAGTAGTCCCAGTAACGATG AGGCAGCAATGGCTGTCATCATGAGCCTCTTGGAAGCAGATGCGGGGCTGGGTGGCCCCGTTGACTTTAG TGACTTGCCATGGCCGCTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI
ACCN	NM_001243048
Insert Size	1842 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001243048.1, NP_001229977.1
RefSeq Size	2628 bp
RefSeq ORF	1842 bp
Locus ID	11865
UniProt ID	Q9WTL8
Gene Summary	The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with Clock. This heterodimer binds E-box enhancer elements upstream of Period (Per1, Per2, Per3) and Cryptochrome (Cry1, Cry2) genes and activates transcription of these genes. Per and Cry proteins heterodimerize and repress their own transcription by interacting in a feedback loop with Clock/Arntl complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2014] Transcript Variant: This variant (2) lacks several in-frame exons in the coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.

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