Repin1 (NM_001079904) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI425994; Ap4; E430037F08Rik; Zfp-464; Zfp464
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228425 representing NM_001079904 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGGGCGCTGCGTCCCTGGAGGGCACGCGGGAGAGTTTTCTCTGTCATCCGGGGGCTACCGAAGTG TGGGCCGAAGCAGCCGAAGTCTCCCCAGGAACATCCCCAGAAGGAGCTGGAAAAAGCCTCACCCCCAGCT CTTCAGTCTTCAGGAGGAAGAACCGATGCTGGAACAGCGCTGCAGGGGCCCCACGGCCATGGGCCCAGCT CAGCCCTGGCTCTTTTCTGGGCCCTCCCAGGAGTCCTCCCAGCCCGACAGAGGGTTGAGGTACCAGGGCA AATCAGCTCAGCCAAGAGGCCAAACCCCAGGCAAGGTCCATCGTTGTGCCCACTGTCGGAAGCGCTTCCC GGGCTGGGTGGCCCTGTGGCTTCATGCTCGGCGGTGCCAGGCCCGGCTGCCTCTGCCCTGCCATGAATGC AACCAGCGCTTTCGCCACGCCCCCTTCTTAGCGCTGCATCTTCAGGTTCATGCTTCTGCAGTCCCCGACC TGGGTTTCATCTGCCACCTATGTGGGCATAGCTTCCGAGGATGGGTAGCCCTGGTTCTGCATCTGCGGGC TCACTCAGCTTCAAAGCGGCCCATCACTTGCCCTGAATGCGACAGACGCTTCTGGCGACAAAAACAGCTT CGAGCTCATCTGCGAAGGTGCCAGCCCCCTGTCCCTGAGGCCCGGCCCTTCATATGCGGCAACTGTGGCC GGAGCTTTGCCCAATGGGACCAACTGGTTGTTCACAAGCGGGTGCACGTGGCTGAGGCCTTGGAGGAGGC AGCAGCCAAAGCCCTGGGTCCTCGCCCACGAGGACGTCCCGCAGCTCCCAGGCCTGGTGGAGACGCTGTG GACCGCCCCTTCCAGTGTGCCTGCTGCGGCAAGCGTTTCCGCCACAAGCCCAATCTGATCGCCCACCGCC GCGTGCACACTGGTGAGCGACCACACCAGTGCCCAGAGTGCGGGAAGCGTTTCACCAACAAGCCCTACCT GACCTCGCACCGGCGCATACATACCGGCGAGAAGCCCTACCCATGCACCGAGTGTGGCCGCCGCTTCCGC CACAAACCCAACCTGTTGTCGCACAGCAAAATCCACAAGCGCTTGGAGGTCTCAGCGCAGGCTGCCCCAC ACCCCGAGAGTCACCAGATTGCAGCAGAGCCTATGGCACAACCTGCACTTGGGGTGCCCCTGGGGTCCCC GCGGACCCCAGCTGAGGCACCTGCGCTCCTGCATAGCTGCTCCGACTGCGGCCGCAGCTTCCGGCTGGAG CGCTTCCTGCGGCTACACCAGAGGCAGCACACAGGGGAGAGGCCCTTCGCCTGCACAGAGTGTGGCAAGA ACTTCGGCAAGAAGACGCACCTGGTGGCGCACTCACGCGTGCACTCCGGCGAACGTCCCTTCGCCTGCGA GGAGTGTGGTCGCCGTTTCTCACAGGGCAGCCACCTGGCAGCCCACCGGCGAGACCATGCACCAGAGAGG CCCTTCGTGTGCCCGGACTGCGGCAAGGCTTTCCGCCACAAGCCCTACCTGGCTGCGCACCGACGCATCC ACACAGGCGAGAAACCCTATGTGTGTCCCGACTGTGGCAAAGCTTTCAGTCAGAAGTCCAACCTGGTGTC CCACCGGCGCATCCACACAGGCGAGCGGCCCTACGCCTGCCCCGACTGTGATCGTAGCTTCAGTCAGAAG TCCAATCTTATCACACACCGGAAGAGCCACATCCGGGATGGCGCCTTCTGTTGTGCCATCTGTGGCCAGA CCTTTGATGACGAGGACCGACTCTTGATGCACCAGAAGAAGCATGATGCCTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001079904
Insert Size	1803 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001079904.1, NP_001073373.2
RefSeq Size	3103 bp
RefSeq ORF	1803 bp
Locus ID	58887
UniProt ID	Q5U4E2
Gene Summary	Sequence-specific double-stranded DNA-binding protein required for initiation of chromosomal DNA replication. Binds on 5'-ATT-3' reiterated sequences downstream of the origin of bidirectional replication (OBR) and a second, homologous ATT sequence of opposite orientation situated within the OBR zone. Facilitates DNA bending (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) differs in the 5' coding region, uses an alternate in-frame splice site, and uses an alternate start codon, compared to variant 1. The encoded isoform (d) has a distinct and longer N-terminus, compared to isoform a.

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