Enox2 (NM_001271447) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	APK1; Cova1; tNOX
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228416 representing NM_001271447 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGCTGCCTGTGTCTGATCCAGCTGCATGGGCCACAGCAATGAATAATCTTGGAATGGCTCCACTGG GAATTGCTGGACAACCAATTTTACCTGACTTCGATCCTGCCCTTGGGATGATGACTGGAATACCACCAAT AACTCCCATGATGCCGGGTTTGGGCATAGTCCCGCCACCGATTCCTCCAGATATGCCGGTAGCAAAGGAG ATCATACACTGCAAAAGCTGCACGCTCTTCCCTCCCAACCCAAATCTTCCACCACCTGCAACACGAGAAA GGCCACCAGGCTGTAAGACAGTGTTTGTGGGTGGCCTGCCTGAAAATGGGACAGAGCAGATCATTGTGGA AGTGTTTGAACAGTGTGGAGAGATTATTGCTATCCGGAAGAGCAAAAAGAACTTCTGTCACATTCGCTTT GCTGAGGAATACATGGTGGACAAAGCCCTCTATCTGTCTGGTTACCGAATTCGTCTGGGCTCTAGTACTG ACAAGAAGGACACAGGCCGGCTCCATGTTGACTTTGCCCAGGCTCGGGATGACTTATATGAGTGGGAGTG TAAACAGCGTATGCTAGCCAGAGAGGAGCGGCACCGTAGAAGGATGGAAGAAGAAAGAATGCGTCCACCA TCCCCACCTCCAGTGGTCCACTATTCAGATCATGAATGCAGCATTGTTGCTGAAAAACTCAAAGATGATT CCAAATTCTCGGAAGCTGTGCAGACCTTGCTCACCTGGATTGAGAGGGGGGAAGTGAACCGCCGCAGTGC CAACCACTTCTACTCCATGATCCAGTCAGCCAACAGCCATGTCCGCCGCCTGGTAAATGAGAAAGCTACC CATGAGAAAGAGATGGAAGAAGCAAAAGAGAAGTTCAAGCAGGCCCTTTCTGGAATTCTCATTCAATTTG AGCAGATAGTAGCTGTGTACCATTCCGCTTCCAAACAGAAGGCATGGGACCACTTCACAAAAGCACAACG TAAAAACATCAGTGTTTGGTGCAAACAAGCTGAGGAAATTCGCAACATTCATAATGATGAATTAATGGGA ATCAGAAGAGAAGAAGAAATGGAAATGTCTGATGATGAAATAGAAGAGACAACAGAAACAAAAGAAACGG AGGAATCAGCCTTAGTGTCACAGGCAGAAGCTCTGAAGGAGGAAAATGATAGCCTCCGCTGGCAGCTGGA CGCATACCGGAATGAGGTAGAACTGCTCAAACAGGAACAAGGCAAAGCCCACAGGGAGGATGACCCAAAC AAGGAACAGCAGCTGAAACTTCTGCAGCAAGCCCTACAAGGGATGCAACAGCATCTGCTCAAAGTCCAAG AGGAATACAAAAAGAAAGAAGCTGAGCTTGACCGAATCAAAGACGACAATTTACAGGTAGAACAACTGTT GGAAAATTTTCATGAAAAGCAGGAAAACTGTGGTTCTAGACTGTGTGCATCAAGCCAAGAAGGTGAGCAA CCTCTTGAGAAGACCGCAGTCAGCAATCCTGTCAAATCTGAACGTGAGGCACTGCTAGTGGGGATCATCT CCACATTCCTTCACGTCCACCCATTTGGAGCCAGCATTGAATACATCTGTTCCTACTTGAATCGTCTCGA TAATAAGGCAAGCTACCAAATACCCAGCAAATTAACCACTTCTCCACTCTTACCGATCAGCACCAGTGAT GTGGAATCTCTCATGAGTAGACTCCAGCATACCTTCAGACAGGAAATGACTGGAGTTGGAGCCAGCCTGG AGAAGAGATGGAAATTCTGTGGTTTTGAGGGCTTGAAGCTGACCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271447
Insert Size	1797 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271447.1, NP_001258376.1
RefSeq Size	3977 bp
RefSeq ORF	1797 bp
Locus ID	209224
UniProt ID	Q8R0Z2
Gene Summary	May be involved in cell growth. Probably acts as a terminal oxidase of plasma electron transport from cytosolic NAD(P)H via hydroquinones to acceptors at the cell surface. Hydroquinone oxidase activity alternates with a protein disulfide-thiol interchange/oxidoreductase activity which may control physical membrane displacements associated with vesicle budding or cell enlargement. The activities oscillate with a period length of 22 minutes and play a role in control of the ultradian cellular biological clock (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Variants 1, 2, 3, and 4 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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