Repin1 (NM_001079901) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI425994; Ap4; E430037F08Rik; Zfp-464; Zfp464
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228415 representing NM_001079901 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGATAGGGATGTCTTTATTGCTACAGTTTTCTCTGTCATCCGGGGGCTACCGAAGTGTGGGCCGAA GCAGCCGAAGTCTCCCCAGGAACATCCCCAGAAGGAGCTGGAAAAAGCCTCACCCCCAGCTCTTCAGTCT TCAGGCAGAGGAAGAACCGATGCTGGAACAGCGCTGCAGGGGCCCCACGGCCATGGGCCCAGCTCAGCCC TGGCTCTTTTCTGGGCCCTCCCAGGAGTCCTCCCAGCCCGACAGAGGGTTGAGGTACCAGGGCAAATCAG CTCAGCCAAGAGGCCAAACCCCAGGCAAGGTCCATCGTTGTGCCCACTGTCGGAAGCGCTTCCCGGGCTG GGTGGCCCTGTGGCTTCATGCTCGGCGGTGCCAGGCCCGGCTGCCTCTGCCCTGCCATGAATGCAACCAG CGCTTTCGCCACGCCCCCTTCTTAGCGCTGCATCTTCAGGTTCATGCTTCTGCAGTCCCCGACCTGGGTT TCATCTGCCACCTATGTGGGCATAGCTTCCGAGGATGGGTAGCCCTGGTTCTGCATCTGCGGGCTCACTC AGCTTCAAAGCGGCCCATCACTTGCCCTGAATGCGACAGACGCTTCTGGCGACAAAAACAGCTTCGAGCT CATCTGCGAAGGTGCCAGCCCCCTGTCCCTGAGGCCCGGCCCTTCATATGCGGCAACTGTGGCCGGAGCT TTGCCCAATGGGACCAACTGGTTGTTCACAAGCGGGTGCACGTGGCTGAGGCCTTGGAGGAGGCAGCAGC CAAAGCCCTGGGTCCTCGCCCACGAGGACGTCCCGCAGCTCCCAGGCCTGGTGGAGACGCTGTGGACCGC CCCTTCCAGTGTGCCTGCTGCGGCAAGCGTTTCCGCCACAAGCCCAATCTGATCGCCCACCGCCGCGTGC ACACTGGTGAGCGACCACACCAGTGCCCAGAGTGCGGGAAGCGTTTCACCAACAAGCCCTACCTGACCTC GCACCGGCGCATACATACCGGCGAGAAGCCCTACCCATGCACCGAGTGTGGCCGCCGCTTCCGCCACAAA CCCAACCTGTTGTCGCACAGCAAAATCCACAAGCGCTTGGAGGTCTCAGCGCAGGCTGCCCCACACCCCG AGAGTCACCAGATTGCAGCAGAGCCTATGGCACAACCTGCACTTGGGGTGCCCCTGGGGTCCCCGCGGAC CCCAGCTGAGGCACCTGCGCTCCTGCATAGCTGCTCCGACTGCGGCCGCAGCTTCCGGCTGGAGCGCTTC CTGCGGCTACACCAGAGGCAGCACACAGGGGAGAGGCCCTTCGCCTGCACAGAGTGTGGCAAGAACTTCG GCAAGAAGACGCACCTGGTGGCGCACTCACGCGTGCACTCCGGCGAACGTCCCTTCGCCTGCGAGGAGTG TGGTCGCCGTTTCTCACAGGGCAGCCACCTGGCAGCCCACCGGCGAGACCATGCACCAGAGAGGCCCTTC GTGTGCCCGGACTGCGGCAAGGCTTTCCGCCACAAGCCCTACCTGGCTGCGCACCGACGCATCCACACAG GCGAGAAACCCTATGTGTGTCCCGACTGTGGCAAAGCTTTCAGTCAGAAGTCCAACCTGGTGTCCCACCG GCGCATCCACACAGGCGAGCGGCCCTACGCCTGCCCCGACTGTGATCGTAGCTTCAGTCAGAAGTCCAAT CTTATCACACACCGGAAGAGCCACATCCGGGATGGCGCCTTCTGTTGTGCCATCTGTGGCCAGACCTTTG ATGACGAGGACCGACTCTTGATGCACCAGAAGAAGCATGATGCCTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001079901
Insert Size	1797 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001079901.1, NP_001073370.2
RefSeq Size	3177 bp
RefSeq ORF	1797 bp
Locus ID	58887
UniProt ID	Q5U4E2
Gene Summary	Sequence-specific double-stranded DNA-binding protein required for initiation of chromosomal DNA replication. Binds on 5'-ATT-3' reiterated sequences downstream of the origin of bidirectional replication (OBR) and a second, homologous ATT sequence of opposite orientation situated within the OBR zone. Facilitates DNA bending (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes isoform a.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.