Bbs12 (NM_001255992) Mouse Untagged Clone

CAT#: MC228404

Bbs12 (untagged) - Mouse Bardet-Biedl syndrome 12 (human) (Bbs12), transcript variant 2



  "NM_001255992" in other vectors (1)

CNY 5,700.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Gm407; Gm721; Gm1805
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC228404 representing NM_001255992
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCAGAAGGCTTGAACTCTTGCATTGAAGCAGTAGTTTCTCTTCAAGTACCCATACACAATGTATTTG
ACCACATGGACAACACAAGTACAGTTTACAAACTTGAAACAGTTAATGCCACTTTGTGTCCTTTTCTACA
AGACCCTTCAGGTTCTGGGTTGTTACAGGAAAAGCGTGATTTCAAAGATGCTACATCTCCGTTATTGTCC
ACTTACAGTCTTTCTGGGAGACATGCTGAGTCACCCAAATTCTTCAAACCTCAGAATAATCTTGAAACAG
AAAAAAACACATTGCAAGTTTTGAAAAACAATCTATATACAGATTCCTTCTGCAAAAAGTCAGCACTAGC
TCACAGTAGGCATTTTAATAGGACAGATAATAGCCACTGGATAAGCCGACATGATGGATTTCTAGAACAA
CTTGAATCAACTCCGAAAGTACTTAGATGTAATGATTTTGGGGAGTTAGCAGTTGGCTTGAGCCATGGAG
ATCACAGCAGCATGGCATTGGCCAAAGCAGCAGTGAGGTTGCAGTGGCAGAGTCTGTGTCTGCAGCAAGC
CAACTGGATGGCACCCTTTATGTTTGATATTTCAAGACTTCTCACTTGCTGTATTCCAGGTCTACCTGAA
ACTTTTTCCCGTGTTGGTCTAGGATATGTCACTTTTGTTACCATGTCTAGTATTACTCTTATCAAGGAAT
TGCAGGATCAGCCTTTCCGAGTGATTCTCATTGAGGGTGACCTCACAGAGAGTTACCGACACCTGGGATT
TAATAAGTCTGTAAATATTAAGACTAAGTTAGATAGTGGGGAGCTTTCAGAAGATAGTGCAGAAGAACTG
TGGACAAATCATGTGTTACAGGTGTTAATCCAGTTCAATGTGACCCTCATCTTGGTACAAGGAAGTGTAT
CAGAACACTTGACTGAAAAATGCATGCACAGTAAGCGGCTGGTAATTGGGGCAGTGAATGGCAGTGTGTT
GCAGGCATTTGCAGAGGCCACAAGAGCAGTGCCAGTAGCCTATGTTACACAAGTGAATGAAGACTGTGTA
GGCAGCGGGGTCTCTGTGACCTTCTGGATGAGTCCTCATGATATAAACAGGAGCAACAGAATAGCAATCT
TGTTAACAGCAGAAGGAATTAATTTGATTACAGCAGTACTTACTAGTCCAGCAAGTGCTCAGATGGAAAC
CAAGGAAGACAGGTTCTGGTCTTGTGTGTATCGTTTATATCATGCCCTAAAAGAAGAGAAGGTCTTCCTT
GGCGGTGGTGCTGTTGAATTTTTATGTCTTAGCCATCTTCAAATTCTTGCTGAGCAATCTTTAAATAGAG
GAAACCATGCTTGTTTAGGATGGCTTCCTGATTCTTCCTCTTGGATGGCCTCATCTCTGTCCGTCTACAG
ACCTACTGTGCTGAAGTCCCTGGCAGGTGGGTGGCATGAATTCCTGTCAGCTATCATGTGTAACACTGCC
ACTCACCCATCAGCAGTGGAAGCTAGGACATTCATTCAACAACATGTACAAAATGCCATTGACTCTGGCT
CCCCTTCATCTTACATCTTGAGTGAATATAGTAAACTAAGTAGTGGAGTTTTTCATTCAGGTATTTCAGA
TAACCTGGAGCTGGTTCCAAGAGTTTATGATACTGTTACACCAAAGATTGAAGCATGGCGCCGAGCATTG
GATGTAGTGCTCTTAGTGCTTCAGACAGACAGTGAAATAATTACTGGACTTGTACACACAGAGATGAATT
CACAGGAATTAGATGGAGTTTTATTTTTGTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001255992
Insert Size 1782 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001255992.1, NP_001242921.1
RefSeq Size 2347 bp
RefSeq ORF 1782 bp
Locus ID 241950
Gene Summary Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Involved in adipogenic differentiation.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) has an alternate splice site, which results in a downstream AUG start codon, compared to variant 1. The resulting isoform (2) is shorter at the N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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