Bbs12 (NM_001255992) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Gm407; Gm721; Gm1805
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228404 representing NM_001255992 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGAAGGCTTGAACTCTTGCATTGAAGCAGTAGTTTCTCTTCAAGTACCCATACACAATGTATTTG ACCACATGGACAACACAAGTACAGTTTACAAACTTGAAACAGTTAATGCCACTTTGTGTCCTTTTCTACA AGACCCTTCAGGTTCTGGGTTGTTACAGGAAAAGCGTGATTTCAAAGATGCTACATCTCCGTTATTGTCC ACTTACAGTCTTTCTGGGAGACATGCTGAGTCACCCAAATTCTTCAAACCTCAGAATAATCTTGAAACAG AAAAAAACACATTGCAAGTTTTGAAAAACAATCTATATACAGATTCCTTCTGCAAAAAGTCAGCACTAGC TCACAGTAGGCATTTTAATAGGACAGATAATAGCCACTGGATAAGCCGACATGATGGATTTCTAGAACAA CTTGAATCAACTCCGAAAGTACTTAGATGTAATGATTTTGGGGAGTTAGCAGTTGGCTTGAGCCATGGAG ATCACAGCAGCATGGCATTGGCCAAAGCAGCAGTGAGGTTGCAGTGGCAGAGTCTGTGTCTGCAGCAAGC CAACTGGATGGCACCCTTTATGTTTGATATTTCAAGACTTCTCACTTGCTGTATTCCAGGTCTACCTGAA ACTTTTTCCCGTGTTGGTCTAGGATATGTCACTTTTGTTACCATGTCTAGTATTACTCTTATCAAGGAAT TGCAGGATCAGCCTTTCCGAGTGATTCTCATTGAGGGTGACCTCACAGAGAGTTACCGACACCTGGGATT TAATAAGTCTGTAAATATTAAGACTAAGTTAGATAGTGGGGAGCTTTCAGAAGATAGTGCAGAAGAACTG TGGACAAATCATGTGTTACAGGTGTTAATCCAGTTCAATGTGACCCTCATCTTGGTACAAGGAAGTGTAT CAGAACACTTGACTGAAAAATGCATGCACAGTAAGCGGCTGGTAATTGGGGCAGTGAATGGCAGTGTGTT GCAGGCATTTGCAGAGGCCACAAGAGCAGTGCCAGTAGCCTATGTTACACAAGTGAATGAAGACTGTGTA GGCAGCGGGGTCTCTGTGACCTTCTGGATGAGTCCTCATGATATAAACAGGAGCAACAGAATAGCAATCT TGTTAACAGCAGAAGGAATTAATTTGATTACAGCAGTACTTACTAGTCCAGCAAGTGCTCAGATGGAAAC CAAGGAAGACAGGTTCTGGTCTTGTGTGTATCGTTTATATCATGCCCTAAAAGAAGAGAAGGTCTTCCTT GGCGGTGGTGCTGTTGAATTTTTATGTCTTAGCCATCTTCAAATTCTTGCTGAGCAATCTTTAAATAGAG GAAACCATGCTTGTTTAGGATGGCTTCCTGATTCTTCCTCTTGGATGGCCTCATCTCTGTCCGTCTACAG ACCTACTGTGCTGAAGTCCCTGGCAGGTGGGTGGCATGAATTCCTGTCAGCTATCATGTGTAACACTGCC ACTCACCCATCAGCAGTGGAAGCTAGGACATTCATTCAACAACATGTACAAAATGCCATTGACTCTGGCT CCCCTTCATCTTACATCTTGAGTGAATATAGTAAACTAAGTAGTGGAGTTTTTCATTCAGGTATTTCAGA TAACCTGGAGCTGGTTCCAAGAGTTTATGATACTGTTACACCAAAGATTGAAGCATGGCGCCGAGCATTG GATGTAGTGCTCTTAGTGCTTCAGACAGACAGTGAAATAATTACTGGACTTGTACACACAGAGATGAATT CACAGGAATTAGATGGAGTTTTATTTTTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001255992
Insert Size	1782 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001255992.1, NP_001242921.1
RefSeq Size	2347 bp
RefSeq ORF	1782 bp
Locus ID	241950
Gene Summary	Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Involved in adipogenic differentiation.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) has an alternate splice site, which results in a downstream AUG start codon, compared to variant 1. The resulting isoform (2) is shorter at the N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.