Npnt (NM_001287102) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1110009H02Rik; AA682063; AI314031; Nctn; POEM
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228399 representing NM_001287102 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGTGCTCCTAGCGGCGGTGCTCGCGTCCTCGCTCTACCTGCAGGTGGCTGCCGACTTCGACGGGA GGTGGCCCAGGCAAATAGTTTCTTCAATCGGCCTATGTCGCTATGGAGGCAGGATTGACTGCTGCTGGGG CTGGGCCCGCCAGTCCTGGGGACAGTGCCAACCTGTGTGTCAACCACAGTGCAAACACGGAGAGTGCGTC GGGCCAAACAAGTGCAAATGTCACCCTGGATTTGCTGGGAAAACCTGCAACCAAGATGAGTCCTTCCACC CAACTCCTCTTGACCAAGGCAGTGAACAGCCTCTTTTCCAACCCCCGGATCATCAAGCCACAAATGTACC TTCAAGGGATTTAAACGAGTGTGGCCTGAAGCCTCGGCCCTGTAAGCACAGGTGCATGAACACTTTCGGC AGCTACAAGTGCTACTGTCTCAACGGATACATGCTGCTGCCAGACGGGTCCTGCTCAAGTGCCCTATCGT GTTCCATGGCAAACTGTCAGTATGGCTGTGACGTGGTCAAAGGACAGGTCCGATGTCAGTGTCCATCCCC TGGCCTGCAGCTAGCTCCCGATGGGAGGACCTGTGTGGATATTGATGAATGTGCGACTGGAAGAGTCTCC TGCCCTCGATTTAGGCAGTGTGTCAACACGTTTGGGAGCTACATCTGCAAGTGTCACACTGGTTTCGACC TCATGTACATTGGAGGCAAATATCAGTGCCATGACATCGACGAGTGCTCTCTTGGACAGCACCAGTGTAG CAGCTATGCCCGGTGTTACAACATACATGGGTCCTACAAGTGCCAATGTAGAGATGGATACGAGGGGGAT GGACTGAACTGTGTGTATATCCCCAAAGTCATGATTGAACCTTCAGGTCCAATCCATATGCCAGAAAGAA ATGGTACAATCTCAAAGGGTGATGGAGGACATGCGAATAGGATTCCTGATGCTGGAAGTACAAGGTGGCC CCTGAAGACACCATATATTCCTCCTGTCATTACCAACAGGCCTACTTCCAAGCCAACAACAAGACCTACA CCAAACCCAACACCACAGCCTACTCCACCACCTCCACCACCCCTCCCGACAGAGCCCAGAACAACTCCAC TACCACCAACCCCAGAAAGGCCATCTACCAGACCCACCACTATAGCACCTGCTACCAGTACCACTACACG AGTAATTACGGTTGACAACAGGATACAGACGGATCCTCAGAAACCCAGAGGAGATGTGTTCATTCCACGG CAGCCGACAAATGACCTGTTTGAGATATTTGAAATCGAAAGAGGGGTCAGCGCGGATGAGGAAGTAAAGG ACGACCCAGGTATTCTCATACACAGCTGCAATTTTGACCATGGACTCTGTGGATGGATCAGAGAAAAAGA TAGTGACTTGCACTGGGAGACAGCCAGGGACCCAGCAGGTGGACAGTATCTCACAGTGTCTGCAGCCAAA GCCCCGGGGGGAAAAGCCGCTCGCTTGGTGTTACGTCTCGGCCACCTCATGCATTCAGGGGACCTGTGCC TGTCCTTTAGGCACAAGGTGACTGGGCTGCACTCGGGCACACTGCAGGTGTTTGTGAGGAAACACGGTAC CCACGGAGCAGCCCTGTGGGGAAGAAATGGTGGCCATGGCTGGAGGCAAACCCAGATCACCTTGCGAGGG GCTGACGTCAAAAGCGTCATCTTCAAAGGTGAAAAAAGGCGTGGTCACACGGGGGAGATTGGATTGGATG ATGTGAGCTTGAAAAGAGGTCGCTGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001287102
Insert Size	1779 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001287102.1, NP_001274031.1
RefSeq Size	4712 bp
RefSeq ORF	1779 bp
Locus ID	114249
UniProt ID	Q91V88
Gene Summary	Functional ligand of integrin alpha-8/beta-1 in kidney development. Regulates the expression of GDNF with integrin alpha-8/beta-1 which is essential for kidney development. May also play a role in the development and function of various tissues, regulating cell adhesion, spreading and survival through the binding of several integrins.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) lacks an in-frame exon in the 5' coding region compared to variant 3. The encoded isoform (d) is shorter than isoform c. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.