Atg16l1 (NM_001205392) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1500009K01Rik; Apg16l; Atg16l; WDR30
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228389 representing NM_001205392 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGTCGGGCCTGCGCGCCGCAGACTTTCCCCGCTGGAAGCGTCACATCGCGGAGGAACTGAGGCGCC GGGACCGACTGCAGAGGCAGGCGTTCGAGGAGATCATTCTGCAGTATACCAAGTTGCTGGAAAAGTCAGA TCTTCATTCAGTATTGACCCAGAAACTACAAGCAGAAAAGCATGACATGCCAAATAGGCATGAAATAAGT CCTGGACATGATGGTGCGTGGAATGATAGTCAACTACAAGAAATGGCCCAGTTGAGGATCAAACACCAGG AAGAGCTGACCGAACTGCACAAGAAGCGTGGGGAGTTAGCTCAGTTGGTGATTGACCTGAACAACCAAAT GCAGCAGAAGGACAAGGAGATACAGATGAATGAAGCAAAGATTTCGGAGTATTTACAGACCATCTCTGAC CTGGAGACAAACTGCCTGGACCTGCGCACCAAACTGCAGGACCTCGAGGTAGCCAACCAGACCCTGAAGG ATGAGTATGACGCCCTGCAGATTACTTTTACTGCCCTAGAAGAGAAACTGAGGAAAACTACTGAGGAGAA CCAGGAACTGGTCACCAGATGGATGGCTGAGAAGGCCCAAGAAGCCAATCGCCTCAATGCAGAGAATGAG AAGGACTCCAGGAGGCGTCAAGCACGGCTGCAGAAGGAGCTTGCAGAAGCAGCAAAGGAACCTCTACCTG TTGAACAGGATGATGACATTGAAGTCATTGTGGATGAGACCTCAGACCACACAGAAGAGACCTCTCCCGT CCGAGCTGTCAGCAGAGCAGCTACGAGACGCTCTGTCTCTTCCATCCCAGTCCCCCAGGATATCATGGAC ACTCATCCTGCTTCTGGTAAAGATGTGAGAGTCCCAACTACTGCCTCGTATGTCTTCGATGCGCATGACG GAGAGGTCAACGCAGTGCAGTTCAGTCCAGGCTCCCGGTTGCTGGCCACTGGAGGCATGGACCGCAGGGT GAAACTTTGGGAAGCATTCGGAGATAAATGTGAATTCAAGGGCTCCCTGTCTGGCAGTAATGCTGGAATT ACAAGCATTGAATTTGATAGTGCTGGAGCTTACCTATTAGCAGCTTCAAATGATTTTGCAAGCCGAATCT GGACTGTGGATGATTATCGATTACGGCACACACTCACAGGCCACAGCGGGAAAGTCCTCTCTGCCAAGTT CCTGCTGGACAATGCACGGATTGTCTCAGGAAGTCACGACCGGACCCTCAAACTCTGGGATCTCCGCAGC AAAGTCTGCATAAAAACAGTGTTTGCAGGATCCAGCTGCAATGACATTGTTTGCACTGAACAATGTGTAA TGAGTGGACATTTTGACAAGAAAATTCGTTTCTGGGATATCCGGTCAGAGAGTGTGGTCCGAGAGATGGA ACTGTTAGGGAAGATCACTGCTCTGGACCTAAACCCTGAGAGAACTGAGCTCCTGAGCTGCTCCCGTGAT GACCTGCTAAAAGTCATCGACCTCCGGACAAATGCAGTCAAACAGACATTCAGTGCGCCTGGATTCAAAT GCGGCTCTGACTGGACCCGGGTTGTCTTCAGCCCTGATGGCAGTTACGTGGCAGCAGGCTCAGCCGAGGG TTCTCTTTATGTCTGGAGTGTGCTGACAGGGAAAGTGGAGAAGGTTCTTTCAAAACAGCACAGCTCTTCT ATCAATGCGGTGGCGTGGGCCCCCTCGGGCTTACATGTTGTCAGTGTGGACAAAGGAAGCAGAGCTGTGC TGTGGGCACAGCCTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001205392
Insert Size	1767 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001205392.1, NP_001192321.1
RefSeq Size	3075 bp
RefSeq ORF	1767 bp
Locus ID	77040
Gene Summary	Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane (PubMed:18849966, PubMed:12665549, PubMed:24954904, PubMed:24553140, PubMed:23392225). Regulates mitochondrial antiviral signaling (MAVS)-dependent type I interferon (IFN-I) production (By similarity). Negatively regulates NOD1- and NOD2-driven inflammatory cytokine response (PubMed:24238340). Instead, promotes with NOD2 an autophagy-dependent antibacterial pathway. Plays a role in regulating morphology and function of Paneth cell.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3, also known as Apg16Lalpha) lacks two consecutive exons in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (3, also known as Apg16Lalpha) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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