Fzd5 (NM_001042659) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	5330434N09Rik; AI427138; Fz-5; Fz5; mFz5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228382 representing NM_001042659 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCGACCCGACCCGTCTGCGCCTCCCTCTCTTCTGCTGCTGTTGCTGGCGCAGCTGGTGGGCCGGG CAGCGGCCGCCTCCAAGGCCCCGGTGTGCCAGGAAATCACGGTGCCCATGTGCCGAGGCATCGGCTACAA CCTGACGCACATGCCCAACCAGTTCAACCATGACACGCAGGACGAAGCAGGCCTGGAGGTGCACCAATTC TGGCCGCTTGTGGAGATCCACTGCTCACCGGACCTGCGCTTCTTCCTGTGCTCTATGTACACGCCCATCT GTTTGCCTGACTACCACAAGCCGCTACCACCGTGCCGTTCCGTGTGCGAGCGCGCCAAGGCCGGCTGCTC GCCGCTCATGCGCCAGTACGGCTTCGCCTGGCCCGAGCGCATGAGCTGCGACCGCCTCCCTGTGCTGGGC GGCGACGCCGAGGTTCTGTGTATGGATTATAACCGAAGCGAAGCCACCACCGCGTCCCCTAAGTCCTTCC CGGCCAAACCTACACTCCCAGGACCACCAGGGGCGCCATCTTCCGGGGGCGAGTGCCCCTCGGGAGGCCC ATCCGTGTGCACGTGCCGCGAGCCCTTCGTGCCCATCCTGAAGGAGTCACACCCACTCTACAACAAGGTG CGCACCGGCCAAGTGCCCAACTGCGCGGTGCCCTGCTACCAGCCGTCCTTCAGCCCGGACGAGCGCACAT TCGCCACCTTCTGGATTGGCCTGTGGTCTGTGCTGTGCTTCATCTCCACGTCCACCACCGTTGCCACCTT CCTCATTGACATGGAACGATTCCGCTACCCTGAGCGCCCCATCATCTTCTTGTCTGCGTGCTACCTGTGT GTGTCACTGGGATTCTTGGTGCGCCTGGTAGTGGGCCATGCCAGCGTCGCTTGCAGCCGTGAGCACAGCC ACATTCACTATGAGACTACCGGCCCTGCGCTGTGCACGGTTGTCTTCCTCTTAGTCTATTTCTTTGGCAT GGCCAGCTCCATCTGGTGGGTCATCCTGTCGCTCACCTGGTTCTTGGCGGCTGGCATGAAGTGGGGCAAT GAAGCCATCGCAGGTTATGCACAGTACTTCCACCTTGCTGCCTGGCTCATCCCCAGTGTCAAGTCCATTA CGGCGCTGGCACTGAGCTCGGTGGACGGGGACCCAGTGGCTGGCATCTGCTATGTGGGCAACCAAAACCT GAACTCACTACGAGGCTTTGTCTTGGGCCCACTGGTGCTGTACCTGTTGGTGGGCACGCTCTTCCTTCTG GCAGGCTTCGTGTCACTCTTCCGCATCCGGAGCGTCATCAAGCAGGGTGGCACTAAGACGGACAAGCTAG AGAAGCTCATGATCCGCATCGGCATCTTCACCCTGCTCTACACGGTGCCAGCCAGCATCGTGGTGGCCTG CTACCTGTATGAGCAGCACTACCGGGAGAGCTGGGAGGCAGCCCTCACCTGCGCGTGTCCGGGACCGGAC GCTGGCCAGCCACGCGCCAAACCCGAGTACTGGGTGCTCATGCTCAAGTACTTCATGTGCCTGGTGGTGG GCATCACGTCGGGAGTCTGGATCTGGTCCGGCAAGACTCTGGAGTCTTGGCGGCGGTTCACCAGCCGCTG CTGCTGCAGCTCTCGGCGGGGCCACAAGAGCGGTGGCGCTATGGCCGCAGGAGACTATGCGGAGGCCAGC GCCGCGCTCACCGGCAGGACCGGGCCGCCTGGCCCCACCGCCGCATACCACAAGCAAGTGTCCCTGTCGC ACGTATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001042659
Insert Size	1758 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001042659.1, NP_001036124.1
RefSeq Size	6823 bp
RefSeq ORF	1758 bp
Locus ID	14367
UniProt ID	Q9EQD0
Gene Summary	Receptor for Wnt proteins (PubMed:11092808, PubMed:18230341). Can activate WNT2, WNT10B, WNT5A, but not WNT2B or WNT4 (in vitro); the in vivo situation may be different since not all of these are known to be coexpressed (PubMed:11092808). In neurons, activation of WNT7A promotes formation of synapses (By similarity). Functions in the canonical Wnt/beta-catenin signaling pathway (PubMed:18230341). The canonical Wnt/beta-catenin signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes (PubMed:18230341). A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable). Plays a role in yolk sac angiogenesis and in placental vascularization (PubMed:11092808).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein.

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