Grk6 (NM_001286064) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Gprk6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228380 representing NM_001286064 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTCGAGAACATCGTAGCGAACACGGTGCTACTCAAGGCCCGGGAAGGTGGTGGCGGGAATCGCA AAGGCAAGAGCAAGAAATGGCGCCAGATGCTGCAGTTCCCCCATATCAGCCAGTGTGAGGAGCTTCGACT CAGCCTTGAGCGTGACTACCACAGCCTATGTGAGCGCCAGCCCATTGGGCGCCTGTTATTTCGTGAGTTC TGTGCTACGAGACCTGAGCTGACCCGGTGTACTGCCTTCCTGGATGGGGTGTCTGAATATGAGGTGACCC CTGATGAGAAGCGGAAAGCATGTGGGCGCCGACTAATGCAGAACTTTCTGAGCCACACGGGTCCTGACCT CATCCCTGAAGTTCCACGGCAGCTGGTGAGTAACTGTGCCCAGCGGCTAGAGCAGGGACCCTGCAAAGAC CTCTTCCAGGAGCTGACCCGGCTGACCCACGAGTACCTGAGCACGGCCCCTTTTGCCGACTACCTCGACA GCATCTACTTCAACCGTTTTCTGCAGTGGAAGTGGCTGGAAAGGCAACCAGTGACCAAAAACACCTTCAG GCAGTACCGAGTCCTGGGCAAAGGTGGCTTTGGGGAGGTGCGGGCAACAGGCAAGATGTACGCATGCAAG AAACTGGAAAAGAAGCGGATAAAGAAGCGAAAGGGGGAGGCCATGGCTCTCAACGAGAAACAGATCTTGG AGAAAGTGAACAGTAGGTTTGTAGTGAGCTTAGCCTACGCCTATGAGACCAAGGATGCACTGTGCCTGGT GCTGACATTGATGAATGGAGGTGACCTAAAGTTCCACATCTACCACATGGGCCAGGCTGGCTTTCCTGAA GCACGTGCTGTCTTCTATGCTGCTGAGATCTGCTGTGGCCTGGAAGACCTGCACCGGGAACGCATTGTGT ACAGGGATCTAAAGCCAGAGAATATCCTTCTGGATGACCATGGCCACATTCGGATCTCGGACCTGGGACT GGCCGTGCATGTGCCTGAGGGCCAGACCATCAAAGGCCGTGTGGGCACTGTGGGCTACATGGCTCCAGAG GTGGTGAGGAATGAGCGCTACACGTTCAGTCCTGACTGGTGGGCGCTAGGCTGCCTCCTGTACGAGATGA TCGCAGGTCAGTCGCCCTTCCAGCAGAGGAAGAAGAAGATAAAGCGCGAAGAGGTGGAGCGGCTGGTCAA GGAAGTGGCCGAGGAGTACACAGACCGCTTTTCCTCACAGGCGCGCTCACTCTGTTCTCAGCTTCTTAGC AAGGACCCTGCTGAGCGCCTGGGGTGTCGTGGAGGTGGCGCCCGTGAGGTAAAGGAGCACCCCCTTTTCA AGAAACTGAATTTCAAGCGGCTGGGAGCTGGCATGCTAGAGCCACCTTTTAAGCCTGATCCCCAGGCTAT TTATTGCAAGGATGTCCTGGACATTGAACAGTTCTCTACAGTTAAAGGTGTGGATCTGGAGCCCACAGAC CAAGACTTCTACCAGAAGTTTGCCACAGGCAGTGTGTCCATCCCCTGGCAGAATGAGATGGTGGAGACCG AGTGCTTCCAGGAACTCAATGTCTTTGGGCTGGATGGGTCTGTTCCCCCAGACCTGGACTGGAAGGGCCA GCCCACTGCACCCCCCAAGAAGGGATTGCTACAGAGACTCTTCAGTCGCCAAAGGATTGCTGTGGGAACT GCAGCGACAGTGAGGAAGAGCTCCCCACCCGCCTCTAGCCCCCAGGCCGAGGCCCCCACCGGCGGTTGGC GGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286064
Insert Size	1755 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286064.1, NP_001272993.1
RefSeq Size	3001 bp
RefSeq ORF	1755 bp
Locus ID	26385
UniProt ID	O70293
Gene Summary	Specifically phosphorylates the activated forms of G protein-coupled receptors. Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their desensitization. Seems to be involved in the desensitization of D2-like dopamine receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis (By similarity). Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor, LRP6 during Wnt signaling (in vitro) (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (e) is shorter, compared to isoform a.

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