Snap91 (NM_001277985) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	91kDa; AP180; F1-20; mKIAA0656
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228376 representing NM_001277985 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGGCCAAACGCTCACGGATCGGATCGCCGCCGCTCAGTACAGCGTGACTGGCTCTGCTGTAGCAA GAGCAGTCTGCAAAGCCACCACTCATGAGGTGATGGGCCCCAAGAAGAAGCACCTGGACTATTTGATCCA GGCTACCAATGAGACCAATGTCAATATCCCTCAGATGGCCGACACCCTCTTTGAGCGGGCGACAAACAGT AGCTGGGTGGTGGTATTTAAAGCTTTAGTGACCACACACCATCTCATGGTGCATGGAAATGAGAGATTTA TTCAGTATTTGGCCTCTAGGAATACGCTATTCAATCTCAGCAACTTTCTAGATAAAAGTGGATCCCACGG TTATGATATGTCTACGTTCATACGGCGTTACAGTAGATACTTGAATGAAAAGGCTTTCTCCTACAGACAG ATGGCATTTGACTTTGCCAGAGTGAAGAAAGGGGCTGACGGTGTCATGAGGACGATGGTTCCTGAAAAGC TCCTGAAGAGTATGCCAATCCTGCAGGGGCAGATCGATGCACTGCTGGAGTTTGATGTGCATCCAAATGA ACTAACCAATGGTGTCATAAATGCTGCATTTATGCTTCTTTTCAAAGATCTTATCAAACTGTTTGCTTGC TACAATGACGGCGTCATTAACTTACTTGAAAAATTTTTCGAGATGAAGAAAGGTCAATGCAAAGACGCGC TAGAAATTTACAAGCGATTTCTAACTAGAATGACGAGGGTGTCTGAATTCCTCAAGGTCGCCGAGCAAGT TGGTATTGATAAAGGTGACATTCCCGACCTCACACAGGCTCCCAGCAGTCTTATGGAGACCCTTGAACAA CATCTAAATACCCTAGAAGGAAAGAAACCTGGAAACAAATCTGGTGCTCCCTCTCCACTAAGTAAGTCTT CTCCAGCCACAACTGTTACATCTCCTAATTCTACACCAGCTAAAACTATCGACACATCCCCGCCAGTTGA CATATTTGCAACAGCATCCGCGGCTGCCCCAGTCAGCTCTGCTAAGCCATCAAGCGATCTCCTTGATCTT CAGCCCGACTTCTCTGGAGCAGCTGCGGGGGCAGCAGCACCTGTAGTGCCTCCTTCTGGGGGTGCGACCG CCTGGGGAGGATTTGGGGGTTCTTTCATGGCCCCTTCTACAACGCCAGTGACTCCAGCTCAGAATAACCT GCTGCAACCCAGTTTCGAGGCAGCTTTTGGAACGACGCCTTCGACTTCAAGCAGCAGCTCTTTTGACCCA TCAGTGTTTGATGGTTTAGGCGATCTTCTGATGCCAACCATGGCACCATCCGGGCAGCCTGCCCCTGTCT CAATGGTCCCACCCAGTCCTGCAATGGCAGCCAGCAAAGGCCTCGGAAGTGACCTTGACTCGTCTCTGGC CAGTTTAGTAGGCAATCTTGGGATTTCTGGTACCACATCAAAAAAGGGAGATCTCCAGTGGAATGCTGGG GAGAAAAAGCTGACTGGTGGAGCCAACTGGCAACCGAAAGTCACTCCAGCCACATGGTCAGCGGGTGTTC CCCCGCAAGGCACTGTTCCACCAACCAGCTCAGTCCCTCCGGGTGCCGGGGCCCCGTCCGTTGGGCAACC TGGAGCAGGATTTGGAATGCCTCCTTCAGGGACAGGCATGACCATGATGTCTCAGCAGCCAGTCATGTTT GCACAGCCCATGATGAGGCCACCCTTTGGAGCTGCAGCTGTGCCCGGCACACAGCTGCAATTTTTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001277985
Insert Size	1749 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001277985.1, NP_001264914.1
RefSeq Size	3386 bp
RefSeq ORF	1749 bp
Locus ID	20616
Gene Summary	Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. Binding of AP180 to clathrin triskelia induces their assembly into 60-70 nm coats.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) lacks multiple exons in the coding region, compared to variant 1. The The resulting isoform (5) lacks two internal segments and has a shorter and distinct C-terminus, compared to isoform 1.

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