Nprl3 (NM_001284359) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Aag; CGTHBA; HS-26; HS-40; m(alpha)RE; Mare; Phg; Prox1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228373 representing NM_001284359 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGACAATACCAGCCCCATCAGCGTGATCCTGGTGAGCTCGGGCAGCAGGGGCAATAAGCTGCTCT TCAGGTACCCCTTCCAGAGGAGCCAGGAGCACCCCGCGTCCCAGACAAATAAACCACGGAGCAGATATGC TGTCAACAACACTGGCGAACATGCTGATGACCAGGACGGTGACTCCAGCAAGCCATGTCCTCCAACCGAT GAGCAATTGGTTGCAGGGTTCTCGGATGTTATTCTGGCAACAATTTTGGCAACCAAATCTGAAATGTGCG GCCAAAAATTTGAACTGAAGATTGATAATGTACGGTTTGTTGGGCACCCAACACTGCTGCAGCATGCTCT GGGGCAGGTCTCCAAAACAGATCCATCTCCAAAGCGGGAAGCTCCGACCATGATTCTTTTTAACGTGGTA TTTGCACTGAGGGCCAATGCTGATCCATCAGTGATAAACTGTCTACACAACTTGTCCCGCCGAATTGCTA CTGTCCTGCAGCATGAGGAGCGCCGCTGCCAGTACCTCACTCGAGAGGCCAAGCTGATCCTAGCACTTCA GGATGAGGTGTCTGCTATGGCTGATGCAAATGAAGGTCCTCAGTCCCCATTCCAGCACATCCTACCCAAG TGCAAGCTGGCTCGGGACCTCAAAGAAGCTTATGACAGCCTGTGCACATCTGGTGTGGTACGGCTTCACA TTAACAGCTGGCTGGAAGTGAGTTTCTGTCTGCCCCACAAGATCCACTATGCAGCTTCAAGTCTGATTCC TCCTGAGGCTATTGAACGGAGCCTGAAAGCCATCCGCCCGTACCATGCCTTGCTACTTCTCAGTGACGAG AAGTCTCTGCTGAGTGAGCTTCCCATTGACTGCTCCCCGGCTCTGGTGCGGGTGATCAAGACCACGTCTG CTGTGAAGAACCTGCAGCAGCTAGCCCAGGATGCCGATCTGGCCTTGCTGCAGGTCTTCCAGTTGGCAGC TCACCTGGTATACTGGGGCAAGGCTGTCATCATCTACCCACTGTGTGAGAACAACGTCTATGTCATGTCT CCCAATGCCAGTGTGTGTCTGTACTCCCCGCTAGCTGAACAGTTCTCCCGGCAGTTCCCGTCCCATGACC TGCCATCTGTCCTGGCCAAGTTTTCCTTGCCTGTTTCTTTATCGGAGTTCAGGAGCCCTCTGGCTCCCCC TGCACAGGAGACCCAGCTCATCCAGATGGTGGTGTGGATGCTGCAGCGCCGGCTCCTCATCCAGCTGCAT ACCTATGTTTGCCTAATGGCCTCACCCAGCGAAGAGGAGCCCCGACTGCGAGAGGATGATGTCCCCTTCA CAGCCAGAGTTGGTGGCCGCAGCCTCAGCACACCCAATGCTCTAAGCTTTGGCTCCCCAACCAGCAGTGA CGACATGACCCTTACCAGCCCCAGTATGGACAACTCAAGTGCTGAGCTGCTCCCCAGTGGGGACTCACCA CTGAACAAGAGGATGACAGAGAACCTGCTGGCTAGCCTCTCAGAGCATGAGCGGGCTGCTATCCTCAATG TGCCTGCAGCCCAAAACCCTGAGGACCTCCGCATGTTTGCCAGGCTCCTTCACTACTTCCGTGGCCGCCA CCATCTGGAGGAGATCATGTACAATGAGAACACTCGGCGCTCCCAGCTACTCATGCTCTTTGACAAATTC CGCAGTGTGCTGGTGGTGACCACCCATGAGGACCCTGTTATTGCCGTCTTCCAGGCACTGCTCACATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001284359
Insert Size	1749 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001284359.1, NP_001271288.1
RefSeq Size	2904 bp
RefSeq ORF	1749 bp
Locus ID	17168
UniProt ID	Q8VIJ8
Gene Summary	As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longest isoform (1).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.