Mtif2 (NM_001282120) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2310038D14Rik; 2410112O06Rik; IF-2(Mt); IF-2mt; IF2(mt)
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228371 representing NM_001282120 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCTGAAATGGAGCAAATTAAAACAGGAAAGAATTAGAGAAAATAAAGATGCTGTGAGAAGGCCTG GGACAGATCCAGCTTTATTAAAACCAAGGTCCCCAGTTGTTACTGTAATGGGCCATGTTGATCATGGGAA AACGACCTTACTTGACAAACTTCGAGAAACTCAAGTTGCAGCGATGGAAGTCGGAGGCATCACTCAACAC ATTGGTGCTTTTCTTGTCTCTCTGCCTTCTGGAGAAAAGATAACTTTTCTTGATACTCCTGGACATGCTG CCTTCTCAGCAATGAGAGCCAGAGGAGCTCAGGTCACCGACATTGTTGTGTTGGTTGTAGCTGCAGATGA TGGGGTAATGAAACAAACTGTGGAATCCATTCAGCATGCAAAAGATGCAGAAGTTCCTATTATCCTTGCA ATCAATAAGTGCGACAAGACAGATGCTGATCCTGAAAAGGTGAAAAAAGAGCTCCTAGCTTACGATGTGG TGTGTGAAGAGTATGGTGGTGATGTTCAAGCAGTGCACGTCTCTGCACTTACGGGCGATAACCTGATGGC TTTGGCAGAAGCAACAATTGCTCTCGCAGAAATCTTGGAACTGAAAGCAGATCCCACCGGTCCAGTGGAA GGAACAGTAATAGAGTCTTTCACAGACAAAGGAAGAGGTCCTGTTACAACAGCTATAATTCAAAGAGGAA CTCTGAGAAAAGGCTCAATTCTAGTTGCTGGGAAGAGTTGGGCAAAAGTTCGACTAATATTTGATGAAAA TGGAAAAATACTTAATGAGGCCTATCCCAGCATGCCAGTGGGAATCATAGGCTGGAGAGACCTTCCTTCT GCAGGAGATGAAATTCTTGAAGTAGAATCTGAGCCAAGGGCCCGTGAAGTTATTGAATGGAGGAAGTCTG AGCAAAAAGAAGAAAAAGGCAAAGATGACCTGAAAATAATGGAAGAAAAGCGAAGGGAACACCAAGAAGC GCATCGGAAAGCCCGTGAGAAGTATGGCAGTCTGCACTGGAAAGAGAGATCATATATAAAGTTCCTTGAA AGGAAACAACAGAGACCCTTAAAGCCCAAAGAAAAGGTAGAAAGGCAATCAAATGTACTTCCTATAATTA TTAAAGGTGACGTTGATGGGTCTGTGGAGGCCATCTTGAACCTTCTGGATACCTACGATGCTTCCCACGA ATGTGAACTCGAATTAGTACATTTTGGATTGGGTGACATCAGTGAAAATGATGTTACCTTTGCTGAGACA TTTGATGGTGTTATTTATGGTTTTAATGTGGAGGCAGGCAGTGCTATTCAGCAGTCAGCTGCACAAAAGG GAGTTAAGATTAAACTTCACAAAATCATCTACCATCTTATTGAAGATTTGCAGGAGGAACTAAGCAGCAG ATTGCCCCACACACTGGAGGAGTACCCAATAGGTGAGGCTTCTATACTAGCTACCTTCACTGTAACAGAA GGGAAGAAAAAAATTCCTGTTGCTGGCTGCAGAGTTCAAAAGGGACAATTAGAAAGACATAAGAAGTTTA AATTAATCCGAAACGGCCAAGTTATTTGGAAGGGATCATTAACCTCTCTGAAACACCATAAAGATGACAT TTCAGTTATAAAAACTGGTATGGACTGTGGTCTTAGTTTAGATGAAGAAAAAGTCGAATTCAAACCGGGA GATCAAGTCATTTGTTATGAAGAAAATAAAGTTCCAACTAAGACTTCCTGGGATCCAGGATTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001282120
Insert Size	1746 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282120.1, NP_001269049.1
RefSeq Size	3103 bp
RefSeq ORF	1746 bp
Locus ID	76784
UniProt ID	Q91YJ5
Gene Summary	One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) differs in its 5' UTR and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus, compared to isoform 1.

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