Pomgnt2 (NM_001289558) Mouse Untagged Clone

CAT#: MC228363

Pomgnt2 (untagged) - Mouse protein O-linked mannose beta 1,4-N-acetylglucosaminyltransferase 2 (Pomgnt2), transcript variant 1



  "NM_001289558" in other vectors (1)

CNY 5,610.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Ago61; C85492; Gtdc2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC228363 representing NM_001289558
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCACCTCTCTGCCGTATTCAACGCCCTCCTGGTATCAGTGCTGGCTGCGGTCCTGTGGAAGCATGTTC
GGTTGCGGGAGCATGCGGCCACGCTAGAGGAGGAGCTGGCCCTTGGACAGCAGTCCCTGGATCCAGTCCT
AGGACTGAAGATCGACTACCCGAAGGCCCTGCAGATCCTCATGGAGGGCGGCACCCACATGGTGTGCACA
GGCCGCACCCACACGGACCGCATCTGCCGCTTCAAGTGGCTCTGCTACTCCAATGAGGCCGAAGAATTCA
TCTTTTTCCATGGCAACTCATCTGTGATGCTGCCCAACCTGGGCTCCCGGCGCTTCCAGCCGGCCCTGCT
GGACCTGTCCACTGTGGAGGACCATAATGCCCAGTACTTCAACTTCGTGGAGCTGCCCGCTGCAGCCCTG
CGCTTCATGCCAAAGCCAGTGTTCGTGCCTGATGTAGCCCTCATCGCCAACCGCTTCAACCCCGATAACC
TCATGCACGTCTTTCATGATGACCTGCTCCCACTCTTCTACACACTGAGGCAGTTCCCCGGCCTGGCCCA
AGAGGCCCGGCTCTTTTTCATGGAGGGATGGGGTGAGGGTGCCCACTTTGACCTCTATAAACTCCTCAGC
CCCAAGCAGCCGCTTCTACGTGCACAGCTCAAGACACTGGGCCGACTGCTCTGCTTTTCCCATGCTTTCG
TGGGCCTGTCCAAGGTTACCACCTGGTACCAATACGGCTTTGTACAGCCCCAGGGCCCAAAAGCCAACAT
CCTGGTCTCTGGCAATGAGATCCGGCAGTTCACGCGCTTCATGACTGAAAGGCTGAACGTGAGCCACGCG
GGGGCGCCCCTGGGGGAGGAATACATTCTGGTCTTCAGTCGCACCCAGAACAGACTCATCCTGAACGAGG
CAGAGCTGTTGCTGGAGCTGGCACAAGAGTTCCAGATGAAGACGGTTACAGTGTCCCTGGAGGACCACAC
CTTTGCTGATGTCGTGCGGCTGGTCAGCAATGCCTCCATGTTGGTCAGCATGCATGGGGCCCAGCTGGTT
ACGGCCCTCTTCCTGCCCCGCGGGGCTACCGTGGTTGAACTCTTCCCTTACGCTGTCAATCCTGACCACT
ACACCCCCTATAAGACGCTGGCCACACTGCCTGGCATGGACCTGCAGTATGTAGCCTGGCGAAACATGAT
CCGGGAGAACACAGTCACACACCCTGAGCGACCGTGGGACCAAGGGGGCATCACCCACCTGGACCGGGCT
GAGCAGGCCCGTATCCTGCAGAGCCGAGAGGTCCCCCGGCATCTCTGTTGCCGGAACCCAGAGTGGCTAT
TCCGAATCTACCAGGACACCAGGGTGGACATCCCATCCCTTATGCAATCCATTCGGCGTGTGGTGAAGGG
CCGGCCGGGGCCACGGAGGCAGAGGTGGGCAATCAGCCTGTACCCGGGCAAGGTGCGGGAGGCTCGATGT
CAGGCGTCAGTGCAGGGTGCCACCGAGGCCCGCCTGTCTGTGTCCTGGCAGATCCCGTGGAACCTCAAGT
ACCTGAAAGTGAGGGAGGTGAAATACGAGGTGTGGCTGCAGGAGCAAGGGGAGAACACGTATGTGCCTTA
CATGCTGACCCTGCAGAACCACACCTTCACAGAGAACATCAAACCTTTTACCACCTACCTGGTGTGGGTC
CGCTGTATATTTAACAGGAGCCTCCTGGGACCTTTTGCAGATGTGCTGGTGTGCAGCACGTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001289558
Insert Size 1743 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001289558.1, NP_001276487.1
RefSeq Size 2526 bp
RefSeq ORF 1743 bp
Locus ID 215494
UniProt ID Q8BW41
Gene Summary O-linked mannose beta-1,4-N-acetylglucosaminyltransferase that transfers UDP-N-acetyl-D-glucosamine to the 4-position of the mannose to generate N-acetyl-D-glucosamine-beta-1,4-O-D-mannosylprotein (By similarity). Involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity (PubMed:24256719).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, 3 and 4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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