Cmah (NM_001284519) Mouse Untagged Clone

CAT#: MC228356

Cmah (untagged) - Mouse cytidine monophospho-N-acetylneuraminic acid hydroxylase (Cmah), transcript variant 3



  "NM_001284519" in other vectors (1)

CNY 5,610.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC228356 representing NM_001284519
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGGACAGGAAACAGACAGCTGAGACCCTGCTGACCCTGTCTCCTGCTGAAGTTGCCAACCTCAAGG
AAGGGATCAATTTTTTTCGAAATAAGACTACTGGGAAAGAGTACATTTTATACAAGGAGAAGGACCATCT
AAAGGCATGCAAGAACCTCTGCAAGCACCAGGGAGGCCTGTTCATGAAAGACATCGAGGATTTAGATGGA
AGGTCCGTTAAATGCACAAAGCACAACTGGAAGTTAGACGTGAGCACCATGAAATATATCAACCCTCCAG
GGAGCTTCTGTCAAGACGAGCTCGTTATTGAAATGGATGAAAACAATGGGCTTTCCCTGGTAGAACTGAA
CCCTCCTAACCCCTGGGACTCTGATCCCAGGTCTCCTGAAGAATTAGCTTTTGGGGAAGTACAGATAACA
TATCTCACTCATGCCTGCATGGACCTCAAGTTGGGAGACAAGCGAATGGTATTTGACCCTTGGTTAATTG
GCCCTGCTTTTGCCCGAGGATGGTGGTTGCTACATGAGCCTCCATCTGACTGGTTGGAGAGGCTGTGCAA
AGCAGACCTCATTTATATCAGCCACATGCACTCAGACCACCTGAGCTACCCTACCCTGAAGCAGCTTTCC
CAGAGACGACCAGACATTCCCATTTATGTTGGCGACACAGAAAGGCCTGTGTTTTGGAACCTGGATCAGA
GTGGCGTCGGGTTAACTAACATCAACGTGGTTCCATTTGGAATATGGCAACAGGTAGACAAAAGTCTGCG
GTTCATGATCTTGATGGACGGCGTTCATCCTGAGATGGACACATGCATTATCGTGGAGTACAAAGGTCAT
AAAATACTCAACACAGTGGACTGCACCAGACCCAATGGGGGAAGGCTTCCTGAGAAAGTTGCTCTAATGA
TGAGTGATTTCGCAGGAGGTGCATCAGGCTTTCCAATGACTTTCAGTGGTGGAAAATTTACTGAGGAATG
GAAAGCCCAGTTCATTAAGGCTGAAAGAAGAAAGCTTCTGAATTACAAAGCTCAGCTGGTGAAGGACCTG
CAGCCCCGAATCTACTGTCCGTTTGCTGGGTACTTTGTGGAGTCTCACCCATCTGACAAGTACATTAAGG
AAACAAACACCAAAAATGACCCAAATCAGCTCAACAATCTTATCAGGAAAAACTCTGACGTGGTGACATG
GACCCCACGACCTGGCGCTGTCCTCGACCTTGGCAGGATGCTGAAGGACCCAACAGACAGCAAGGGCATT
GTGGAGCCTCCAGAGGGGACAAAGATTTACAAGGATTCCTGGGACTTTGGCCCGTACCTGGAGATCTTGA
ATTCTGCTGTCAGAGATGAAATCTTCTGTCATTCATCCTGGATTAAAGAGTACTTCACGTGGGCTGGATT
TAAGAATTACAACCTGGTGGTCAGGATGATTGAAACAGATGAAGATTTCAGCCCTTTTCCTGGAGGGTAC
GACTATCTGGTGGACTTTCTAGATTTATCCTTTCCGAAAGAAAGACCCAGCCGGGAGCATCCTTATGAAG
AAATCCATAGCCGGGTGGATGTCATCAGGTACGTGGTGAAGAACGGCCTGCTGTGGGATGATCTGTATAT
TGGATTCCAGACCCGATTGCTGCGGGACCCTGATATATACCATCATCTGTTTTGGAATCATTTTCAGATA
AAACTCCCTCTAACACCACCCAACTGGAAGTCGTTCCTAATGCACTGTGATTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001284519
Insert Size 1734 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001284519.1, NP_001271448.1
RefSeq Size 9909 bp
RefSeq ORF 1734 bp
Locus ID 12763
UniProt ID Q61419
Gene Summary Sialic acids are components of carbohydrate chains of glycoconjugates and are involved in cell-cell recognition and cell-pathogen interactions. Catalyzes the conversion of CMP-N-acetylneuraminic acid (CMP-Neu5Ac) into its hydroxylated derivative CMP-N-glycolylneuraminic acid (CMP-Neu5Gc), a sialic acid abundantly expressed at the surface of many cells.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (3) represents the longest transcript and encodes the longer isoform (a). Variants 1, 2, and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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