Cmah (NM_001284519) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228356 representing NM_001284519 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGACAGGAAACAGACAGCTGAGACCCTGCTGACCCTGTCTCCTGCTGAAGTTGCCAACCTCAAGG AAGGGATCAATTTTTTTCGAAATAAGACTACTGGGAAAGAGTACATTTTATACAAGGAGAAGGACCATCT AAAGGCATGCAAGAACCTCTGCAAGCACCAGGGAGGCCTGTTCATGAAAGACATCGAGGATTTAGATGGA AGGTCCGTTAAATGCACAAAGCACAACTGGAAGTTAGACGTGAGCACCATGAAATATATCAACCCTCCAG GGAGCTTCTGTCAAGACGAGCTCGTTATTGAAATGGATGAAAACAATGGGCTTTCCCTGGTAGAACTGAA CCCTCCTAACCCCTGGGACTCTGATCCCAGGTCTCCTGAAGAATTAGCTTTTGGGGAAGTACAGATAACA TATCTCACTCATGCCTGCATGGACCTCAAGTTGGGAGACAAGCGAATGGTATTTGACCCTTGGTTAATTG GCCCTGCTTTTGCCCGAGGATGGTGGTTGCTACATGAGCCTCCATCTGACTGGTTGGAGAGGCTGTGCAA AGCAGACCTCATTTATATCAGCCACATGCACTCAGACCACCTGAGCTACCCTACCCTGAAGCAGCTTTCC CAGAGACGACCAGACATTCCCATTTATGTTGGCGACACAGAAAGGCCTGTGTTTTGGAACCTGGATCAGA GTGGCGTCGGGTTAACTAACATCAACGTGGTTCCATTTGGAATATGGCAACAGGTAGACAAAAGTCTGCG GTTCATGATCTTGATGGACGGCGTTCATCCTGAGATGGACACATGCATTATCGTGGAGTACAAAGGTCAT AAAATACTCAACACAGTGGACTGCACCAGACCCAATGGGGGAAGGCTTCCTGAGAAAGTTGCTCTAATGA TGAGTGATTTCGCAGGAGGTGCATCAGGCTTTCCAATGACTTTCAGTGGTGGAAAATTTACTGAGGAATG GAAAGCCCAGTTCATTAAGGCTGAAAGAAGAAAGCTTCTGAATTACAAAGCTCAGCTGGTGAAGGACCTG CAGCCCCGAATCTACTGTCCGTTTGCTGGGTACTTTGTGGAGTCTCACCCATCTGACAAGTACATTAAGG AAACAAACACCAAAAATGACCCAAATCAGCTCAACAATCTTATCAGGAAAAACTCTGACGTGGTGACATG GACCCCACGACCTGGCGCTGTCCTCGACCTTGGCAGGATGCTGAAGGACCCAACAGACAGCAAGGGCATT GTGGAGCCTCCAGAGGGGACAAAGATTTACAAGGATTCCTGGGACTTTGGCCCGTACCTGGAGATCTTGA ATTCTGCTGTCAGAGATGAAATCTTCTGTCATTCATCCTGGATTAAAGAGTACTTCACGTGGGCTGGATT TAAGAATTACAACCTGGTGGTCAGGATGATTGAAACAGATGAAGATTTCAGCCCTTTTCCTGGAGGGTAC GACTATCTGGTGGACTTTCTAGATTTATCCTTTCCGAAAGAAAGACCCAGCCGGGAGCATCCTTATGAAG AAATCCATAGCCGGGTGGATGTCATCAGGTACGTGGTGAAGAACGGCCTGCTGTGGGATGATCTGTATAT TGGATTCCAGACCCGATTGCTGCGGGACCCTGATATATACCATCATCTGTTTTGGAATCATTTTCAGATA AAACTCCCTCTAACACCACCCAACTGGAAGTCGTTCCTAATGCACTGTGATTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001284519
Insert Size	1734 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001284519.1, NP_001271448.1
RefSeq Size	9909 bp
RefSeq ORF	1734 bp
Locus ID	12763
UniProt ID	Q61419
Gene Summary	Sialic acids are components of carbohydrate chains of glycoconjugates and are involved in cell-cell recognition and cell-pathogen interactions. Catalyzes the conversion of CMP-N-acetylneuraminic acid (CMP-Neu5Ac) into its hydroxylated derivative CMP-N-glycolylneuraminic acid (CMP-Neu5Gc), a sialic acid abundantly expressed at the surface of many cells.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) represents the longest transcript and encodes the longer isoform (a). Variants 1, 2, and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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