Carf (NM_001285463) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Als2cr8; ECBRC-FC1; ECBRC-FC2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228338 representing NM_001285463 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCAATATCTCTGAAGAGAAACCCACTGGTGTAAGAGCAGATGTTCTCGAAGACAGCACCAGCAATT ACGGCCTTCGCTCACCAGCATCCCTCGTGCTACCCAAAAAGGCAGGAGCCAGACTTGTTGAAGAACCTTT GCTGGCTCCTCTCCAGCCACTTTCTTGTAACACACCCATGTGGGCCTGCCGTCTTAGGAGTTGTGAGAAA ATTGGAGATTCCTACCGTGGCTACTGTGTAAGTGAGACTGAATTAGAAAGTGTCCTAACTTTTCACAAGC AGCAAACACAGACTGTCTGGGGTACTCGCCAGTCTCCAAGCCCAGCCAAACCTGCCACACGCTTGATGTG GAAGTCTCAGTATGTCCCATATGACGGAATCCCATTTGTCAATGCAGGAAGTCGAGCTGTGGTAATGGAG TGTCAGTATGGACCAAGGAGAAAAGGTTTTCAGTTAAAAAAAATCAGTGAGCAAGAAAGTAGGTCTTGTC ATCTCTATAAAGCTACTTGTCCAGCCAGGATATACATTAAAAAGGTACAGAAATTTCCTGAATATAGAGT ACCTACAGACCCCCAAATTGACAGGAAGATAATCAGACTGGAGCAGGAGAAAGCCTTCACTATGCTGAAG AAGAACCTGATGGATGCTGGTGGTGTACTTCGATGGTATGTGCAGTTACCTACACAGCAAGCTCATCAGT ATCATGAATCAGAGACTCCTGGTGTCCCTTTGTCACCGTCACCTTTTCCTATGTCTCCTCTTGAAGAAGA GGAAGCTATAGTCAGAGATGAGAACTGTGCGTTGCCCTCTCGTCTCCACCCGCAAGTAGCGCATAAGATT CAGGAACTAGTGTCACAGGGAGTAGGACAAGTGTATGCAGTAAGGAAGCAGCTAAGAAAATTTGTGGAAA GAGAACTGTTCAAACCTGATGAGATACCTGAAAGACATAATTTATCTTATTTTCCAACTGTAAATGATAT AAAAAATCATATCCATGAGGTACAGAAATCCTTGAGGACTGGAGATGTGGTATATAACTCAGAGATTATT CCAGCAACGCTTCAGTGGACGACAGATAGTGGGAACATCCTCAGAGAGACTGTAACAGTTACATTTGCAG AAGGAAATTTACTAGGAGAACCGATTCCCAGCAAAATGGGAACAAGTCAGACACAGACTGCTGTGTCTCC AGAGCCACTTTCTTCATTTCCACCCAAAATATTTACACACTTTCAGGCTTTGAAGTTACAGCCAAGACTC TCCTCTCCTGATGGCTCACAAGCTTTGGTATCAGTAGATAGCCATGCATCCTCTAGTCCTCCTGGCCTTG TGGATACTGTAGGAAATGCTGAAGTGGATAACCACTCTGTACTGCTTGGTCAGAGTCAAAACCCTGGGAC AGATACATGCCTAACCCAAGACAACAGCACCTCCTCCTCCACGGGCCACCTCCCAGAATCAGTTCCGAAT CCAGTTGCAGAGGATCAGCTGCTGGAAGGTGAAGATGTTGAGGATGCAGGGAATCCGGAAGGAAGTGTAA ATAGGACTCTGTTGGGAGATGTACAAACCGTTCCAATACAGATCATAGACAGCCGTCCAGTGCTTGTTGA AGAAAGTCTGTCTAAGAACCAAGTTAAACAAGAAACCAATGAGCCAACACTGTCTACAGAAGCAAAAACT TTCCTGGACTGTAAAAAAATATCTGCTACATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001285463
Insert Size	1713 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001285463.1, NP_001272392.1
RefSeq Size	5701 bp
RefSeq ORF	1713 bp
Locus ID	241066
Gene Summary	Acts as a transcriptional activator that mediates the calcium- and neuron-selective induction of BDNF exon III transcription. Binds to the consensus calcium-response element CaRE1 5'-CTATTTCGAG-3' sequence (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) has multiple differences compared to variant 1. These differences result in a distinct 5' UTR and cause translation initiation at an alternate start codon compared to variant 1. The encoded protein (isoform 2) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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