Otud5 (NM_001290536) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AA407879; AI553596; BB114028; DUBA; DXImx46e; Sfc7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228329 representing NM_001290536 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTATTCTCCCCAAAAAGAAGCCGCCACCTCCCGACGCCGACCCGGCCAACGAACCGCCGCCGCCCG GGCCGCTGCCCCCGGCGCCTCGGCGCGGTGCGGGTGTAGGCGTGGGTGGCGGCGGCACGGGTGTGGGCGG AGGAGAGCGCGACCGTGACTCCGGCGTCGTGGGGGCCCGTCCCCGGGCTTCGCCGCCACCTCAGGGCCCG CTCCCGGGGCCGCCTGGTGCTCTTCATCGTTGGGCACTGGCCGTGCCGCCTGGCGCAGTCGCGGGCCCTC GGCCACAGCAGGCTTCTCCACCTCCTTGTGGGGGCCCCGGTGGCCCCGGCGGCGGTCCTGGTGACGCTCT TGGTGCGACAACTGCGGGGGTGGGCGCGGCAGGGGTGGTGGTGGGCGTGGGTGGTACCGTGGGCGTGGGC GGCTGCTGCTCGGGGCCCGGGCACAGCAAGCGGCGGCGTCAAGCTCCCGGCGTTGGCGCAGTTGGCGGGG CCAGTCCGGAACGTGAAGAGGTCGGAGCGGGCTACAACAGTGAAGACGAGTATGAAGCTGCCGCAGCGCG AATCGAGGCCATGGATCCCGCCACTGTAGAACAGCAGGAACACTGGTTTGAAAAGGCCTTGCGGGACAAG AAAGGCTTCATCATCAAGCAGATGAAGGAGGACGGTGCCTGTCTATTTCGGGCTGTAGCTGACCAGGTGT ATGGAGACCAGGACATGCATGAGGTTGTTCGAAAGCATTGCATGGACTATCTGATGAAGAACGCTGATTA CTTCTCCAACTATGTCACAGAAGACTTCACCACCTATATCAACCGGAAGCGGAAAAACAACTGCCATGGC AACCACATTGAAATGCAGGCTATGGCAGAGATGTACAACCGTCCTGTGGAGGTGTATCAATATAGCACAG AACCTATCAACACATTCCATGGGATCCATCAAAATGAAGATGAACCCATCCGTGTCAGCTACCACCGGAA TATCCACTATAATTCAGTGGTGAATCCTAACAAGGCCACTATTGGTGTGGGGCTGGGCCTACCGTCATTT AAGCCAGGGTTTGCAGAGCAGTCCCTGATGAAGAATGCCATAAAGACATCAGAAGAGTCATGGATTGAAC AGCAAATGCTGGAAGACAAGAAACGAGCTACAGACTGGGAGGCCACAAATGAGGCCATAGAGGAGCAGGT GGCTCGAGAATCTTACCTTCAGTGGCTGAGGGATCAAGAGAAACAGGCCCGCCAGGTCCGGGGACCCAGC CAGCCCCGGAAAGCCAGTGCCACATGCAGTTCAGCCACAGCAGCAGCCTCCAGTGGCCTGGAGGAATGGA CTAGTCGGTCCCCACGGCAACGAAGTTCAGCCTCGTCACCTGAGCACCCTGAACTGCATGCCGAGCTAGG CATTAAGCCCCCTTCCCCAGGCACTGTGTTAGCTCTTGCCAAACCTCCTTCACCCTGTGCACCAGGTACA AGCAGTCAGTTCTCAGCAGGGGGTGATCGGGCCACCTCTCCTCTTGTGTCCCTCTACCCTGCTCTGGAGT GCCGGGCCCTCATCCAGCAGATGTCCCCCTCTGCCTTTGCAGGTCTGAATGATTGGGACGATGATGAGAT CCTAGCATCGGTGCTGGCAGTGTCCCAACAGGAATACCTAGACAGTATGAAGAAAAACAAAGTGCACAGA GAGCCACCCCCAGACAAGAGTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290536
Insert Size	1704 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290536.1, NP_001277465.1
RefSeq Size	4262 bp
RefSeq ORF	1704 bp
Locus ID	54644
Gene Summary	Deubiquitinating enzyme that functions as negative regulator of the innate immune system. Acts via TRAF3 deubiquitination and subsequent suppression of type I interferon (IFN) production. Has peptidase activity towards 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Can also cleave 'Lys-11'-linked ubiquitin chains (in vitro) (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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