Prc1 (NM_001285998) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	D7Ertd348e
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228328 representing NM_001285998 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGAGAAGTGAGGTGCTAGCAGATGAGTCTATCACATGCCTGCAGAAAGCTCTAACTCACCTCCGGG AAATATGGGAACTAATTGGGATTCCAGAGGAGCAGAGGCTACAAAGAACCGAGGTTGTAAAGAAGCATAT AAAGGATCTTCTGGATAGAATGATTGCTGAAGAGGAGAGCCTGAGGGAAAGGCTGCTCAAAAGCATATCC ATCTGTCAGAAGGAGCTGAGTACCCTGTGCAGTGAGCTACAAGTGAAGCCATTTCAGGAAGAAAAAGATA CAACCATCTTGCAACTAGAAAAAGATTTGCGCACTCAAGTAGAATTGATGAGAAAACAGAAAAAGGAGAG AAAGCAGGAACTGAAGCTACTTCAGGAACAGGAGCAAGAACTCCGTGACATCCTCTGTATGCCGCCCTGT GATGTGGACAGCACCTCTGTCCCCACCTTAGAAGAGCTGAAGCTATTCCGACAGCGTGTGGCAACGCTGA GGGAGACAAAGGAATCGAGGCGTGAAGAATTTGTCAACATTAAGAAACAAATCATATTGTGTATGGAAGA ATTAGAACACTCTCCAGATACAAGCTTTGAAAGAGATGTGGTGTGTGAAGATGAAAGTGCTTTTTGTTTA TCACTGGAGAACATTGCAACATTACAGAAGTTGCTGAAGCAGCTGGAAATGAAAAAATCACAAAATGAAG CAGAATGTGAGGGGCTCCGCACTCAAATCCGAGAGCTCTGGGATAGGTTACAAATACCTGAAGAAGAGAG AGAGCCTGTGGAGGCAATTATGACTGGATCAAAAACCAAAATCAGGAATGCGCTGAAATTAGAAGTGGAT CGGTTAGAAGAACTGAAAATGCAAAACATAAAGCAAGTGATTGAGAAAATCCGAGTGGAGCTGGCTCAAT TCTGGGACCAGTGTTTTTATAGCCAGGAACAGAGGCAGGCTTTTGCCCCTTACTATTCTGAGGACTACAC AGAAAACCTGCTCCATCTTCATGATGCCGAGATTGTACGGTTAAGAAACTACTATGACGTTCACAAGGAG CTGTTCCAAGGCGTCCAGAAGTGGGAAGAGAGCTGGAAACTTTTCCTAGAGTTTGAGAGAAAAGCTTCAG ATCCAGGTCGATTTACAAACCGAGGGGGAAATCTTTTAAAAGAAGAAAAGGAACGAGCAAAGCTCCAGAA AACACTCCCTAAGCTGGAAGAGGAGTTGAAAGCACGGATTGAACAGTGGGAACAGGAGCACTCAACAGCA TTTGTGGTGAATGGGCAGAAATTCATGGAGTATGTTACAGAACAGTGGGAATTACATCGGCTGGAGAAAG AGCGAGCCAAACAGGAGAGGCAACTGAAGAACAAGAAACAGACAGAGGCAGAGATGCTGTATGGCAGTAC TCCCCGGACACCTAGCAAAAGACCAGGACAGACGCCCAAGAAGTCTGGCAAAGTACGCAAGATGAACACT ACCACCATGTCTAGTGCTACACCCAACAGTAGCATTCGGCCTGTCTTTGGTGGATCAGTGTACCGGTCAC CTATGTCTCGACTACCCCCTTCTGGCAGCAAGTCAGTTGTCACTTCTCTGTGTTCTGGAAAGAAAACACC TCGAGCTGCCCAGCTTAGGGCCAACAAGGAGAACTTGGACCTCAATGGCAGCATCCTGAGCGCGAGAACT TTCAAAGGCTTCCAGATCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001285998
Insert Size	1701 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001285998.1, NP_001272927.1
RefSeq Size	2972 bp
RefSeq ORF	1701 bp
Locus ID	233406
Gene Summary	Key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. Required to recruit PLK1 to the spindle. Stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle. Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) uses an alternate splice junction at the 3' end of a coding exon compared to variant 1, that causes a frameshift. The resulting isoform (3) has a shorter and distinct C-terminus compared to isoform 1.

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