Acin1 (NM_001242605) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2610036I19Rik; 2610510L13Rik; Acinus; acinusL; acinusS; Acn; C79325; mKIAA0670
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228326 representing NM_001242605 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACACCAGTGAAAACAGACCTGAAAATGAGGTGCCTGAGCCTCCTCTGCCTGTTGCAGACCAAGTCA GCAATGATGAGCGCCCAGAGGGTGGTGCTGAAGAAGAGGAAAAGAAAGAGAGTTCGATGCCCAAGTCATT CAAGAGGAAAATCTCCGTTGTCTCAGCTACCAAGGGGGTGCAAGCTGGAAACAGTGACACAGAGGGGGGC CAGCCTGGCCGAAAACGCCGTTGGGGAGCCAGCACTGCCGCGACACAGAAGAAACCGTCCATCAGTATCA CCACTGAGTCACTCAAGAGCCTCATCCCCGACATCAAACCCCTGGCGGGGCAGGAGGCTGTTGTGGATCT TCATGCCGATGACTCCCGAATCTCTGAGGATGAGACAGAGCGTAATGGCGACGATGGGACCCATGACAAG GGACTGAAGATATGCCGGACAGTCACTCAGGTAGTACCCGCAGAGGGCCAGGAGAATGGGCAGAGGGAAG AGGAAGAAGAGAAAGAGCCTGAAGCCGAGCTGCCGGCGCCACCCCAGGTGTCAGTGGAGGTTGCCTTGCC CCCACCTGTGGAGCACGAAGTAAAGAAAGTAACATTAGGAGATACCTTAACCCGGAGGTCCATCAGCCAA CAGAAGTCTGGAGTTTCCATTACAATTGATGACCCAGTCCGGACCGCCCAGGTGCCCTCCCCACCCAGGG GCAAGATCAGTAACATTGTCCACATCTCCAACCTGGTTCGTCCCTTCACTTTAGGCCAGCTGAAGGAATT ATTGGGGCGTACAGGAACTTTGGTGGAAGAGGCCTTCTGGATAGACAAGATCAAATCTCATTGCTTTGTG ACGTACTCTACAGTAGAGGAAGCCGTTGCCACCCGCACAGCTCTGCACGGGGTCAAGTGGCCCCAGTCCA ACCCCAAATTCCTTTGTGCTGACTATGCTGAGCAAGATGAGCTGGACTATCACCGGGGACTCTTGGTAGA TCGGCCATCTGAAACTAAGGCAGAGGAACAGGGAGCACCAAGGCCCCTGCATCCCCCACCCCCACCCCCA GTCCAGCCACCGCCCCACCCCCGGGCTGAGCAGCGGGAGCAGGAAAGGGCTGTTCGAGAGCAATGGGCAG AACGGGAACGGGAAATGGAGCGCCGGGAGAGGACTCGGTCTGAGAGAGAATGGGATCGGGACAAAGTTCG AGAGGGACCCCGCTCCCGATCACGGTCCCGTGACCGCCGCCGGAAAGAGCGAGCAAAATCTAAAGAAAAG AAGAGTGAAAAGAAAGAAAAAGCCCAGGAGGAGCCACCTGCCAAGCTGCTGGATGACCTCTTCCGTAAGA CTAAGGCAGCTCCCTGCATCTATTGGCTCCCTCTGACTGAGAGCCAAATTGTTCAGAAGGAGGCAGAGCA AGCTGAACGGGCCAAGGAGCGGGAGAAGCGGCGAAAAGAACGAGAAGAAGAAGAACAAAAGGAACGGGAG AAGGAAGCTGAGCGGGAACGGAACCGGCAGCTAGAACGGGAGAAGAGGAGGGAGCACAGCAGGGAGAGAG AGAGGGACAGGGAGAGAGAGCGGGACAGGGGTGACCGAGAGCGGGAGAGGGAGCGAGACCGAGACCGAGG CAGGGAGAGGGATCGCAGAGACACCAAGCGCCACAGCAGAAGCCGGAGTCGAAGCACACCTGTACGGGAC CGGGGTGGGCGCCGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001242605
Insert Size	1698 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001242605.1, NP_001229534.1
RefSeq Size	2454 bp
RefSeq ORF	1698 bp
Locus ID	56215
UniProt ID	Q9JIX8
Gene Summary	Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Component of the ASAP complexes which bind RNA in a sequence-independent manner and are proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets; ACIN1 confers RNA-binding to the complex. The ASAP complex can inhibit RNA processing during in vitro splicing reactions. The ASAP complex promotes apoptosis and is disassembled after induction of apoptosis. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the activity is different from the established EJC assembly and function. Induces apoptotic chromatin condensation after activation by CASP3. Regulates cyclin A1, but not cyclin A2, expression in leukemia cells (By similarity).[UniProtKB/Swiss-Prot Function]

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