Pdp1 (NM_001290391) Mouse Untagged Clone

CAT#: MC228319

Pdp1 (untagged) - Mouse pyruvate dehyrogenase phosphatase catalytic subunit 1 (Pdp1), transcript variant 5



  "NM_001290391" in other vectors (1)

CNY 5,510.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Gm1024; Ppm2c
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC228319 representing NM_001290391
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGTGTGTGTCCCGGGCCCAGACGAATTGGAATCCCAGTCAGAACGTCCAGTCTGCCACTGTTCTCTG
ATGCCATGCCAGCACCAACTCAACTGTTTTTTCCTCTCGTCCGTAACTGTGAACTGAGCAGAATCTATGG
CACTGCATGTTACTGCCACCACAAACATCTCTGCTGTTCACCACCATACATTCCTCAGAATCGTCTGAGG
TACACACCCCATCCTGCATATGCTACCTTTTGTAGGCCACGGGAGAACTGGTGGCAGTATACTCAAGGAA
GGAGATACGCTTCTACACCGCAGAAATTTTACCTCACACCTCCACAAGTCAACAGCATCCTTAAAGCTAA
TGAATACAGCTTCAAAGTACCAGAATTTGATGGCAAAAATGTCAGTTCCATTCTTGGATTTGACAGCAAT
CAGCTGCCTGCAAATGCACCCATAGAGGACCGGAGAAGTGCAGCAACCTGCTTGCAGACCAGAGGGATGC
TCTTGGGGGTTTTTGATGGTCATGCAGGCTGTGCTTGTTCCCAGGCAGTCAGTGAAAGACTCTTCTATTA
TATTGCTGTTTCCTTGTTGCCCCATGAGACTTTGCTAGAGATTGAAAATGCAGTGGAGAGTGGTCGGGCA
CTGCTACCTATCCTTCAGTGGCACAAGCACCCCAATGATTACTTCAGTAAGGAGGCGTCCAAATTGTATT
TCAACAGCTTGAGGACTTACTGGCAAGAACTCATAGACCTCAATACTGGAGAATCAGCTGATATTGATGT
TAAGGAGGCTTTAATTAATGCTTTCAAGAGACTCGATAATGACATTTCATTGGAGGCTCAAGTTGGTGAT
CCTAATTCTTTTCTAAATTACCTGGTGCTTCGGGTAGCATTTTCTGGGGCTACTGCTTGTGTGGCCCATG
TAGATGGTGTTGACCTCCATGTGGCTAACACTGGTGATAGTAGAGCCATGCTAGGTGTGCAAGAAGAAGA
TGGCTCTTGGTCAGCAGTCACACTCTCTAATGACCACAATGCTCAGAATGAAAGAGAACTAGAGCGTCTG
AAACTGGAACACCCAAAAAATGAGGCCAAGAGCGTGGTAAAGCAGGACCGGCTGCTTGGCTTGCTGATGC
CCTTTAGGGCTTTTGGAGATGTAAAGTTCAAATGGAGCATTGACCTTCAAAAGAGAGTGATAGAGTCTGG
CCCAGACCAGTTGAATGACAATGAATACACCAAGTTTATCCCTCCTAACTATCATACACCTCCTTATCTT
ACTGCTGAGCCAGAGGTAACTTATCACAGATTAAGGCCACAGGATAAATTCCTAGTGTTAGCAACTGATG
GGTTGTGGGAGACTATGCATAGACAGGATGTGGTTAGGATTGTGGGTGAATACTTAACTGGTATGCATCA
CCAACAGCCAATAGCAGTTGGTGGGTACAAGGTGACTCTGGGACAGATGCATGGCCTTTTAACAGAAAGG
AGAGCAAAGATGTCATCAGTGTTTGAGGATCAGAATGCAGCAACCCATCTCATTCGCCATGCTGTAGGCA
ATAATGAATTTGGGGCTGTTGATCATGAACGACTCTCTAAAATGCTTAGTCTTCCTGAAGAGCTTGCTCG
GATGTATAGAGATGACATTACAATCATTGTAGTTCAGTTCAATTCTCATGTTGTAGGGGCATACCAAAAC
CAGGAACAGTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001290391
Insert Size 1692 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001290391.1, NP_001277320.1
RefSeq Size 4184 bp
RefSeq ORF 1692 bp
Locus ID 381511
UniProt ID Q3UV70
Gene Summary Catalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (5) differs in the 5' UTR and the 5' coding region and initiates translation at an alternate start codon, compared to variant 4. Variants 2 and 5 encode the same isoform (b), which has a shorter and distinct N-terminus, compared to isoform d.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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