Iffo1 (NM_001302778) Mouse Untagged Clone

CAT#: MC228315

Iffo1 (untagged) - Mouse intermediate filament family orphan 1 (Iffo1), transcript variant 3



  "NM_001302778" in other vectors (1)

CNY 5,510.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 4733401N06Rik; A930037G23Rik; HOM-TES-103; Iffo
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC228315 representing NM_001302778
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATCCGTTATTTGGTCCCAACCTCTTCCTCCTACAGCAGGAGCAGCAAGGCTTGGCCGGGCCGCTGG
GGGACCCTCTGGGAGGTGACCACTTTGCCGGGGGAGGGGACTTAGCCTCGGCTCCGCTTGCCTCGGCCGG
CCCCTCTGCCTATTCGCCTCCGGGGCCTGGTCCGGCGCCCCCCGCAGCCATGGCTCTCCGCAACGACCTA
GGCTCCAACATCAACGTACTGAAGACCCTGAACCTCCGGTTTCGCTGCTTCCTAGCCAAGGTGCACGAGC
TAGAGCGCCGAAATCGGCTGCTGGAGAAACAGCTGCAGCAGGCTCTAGAGGAGGGTAAGCAGGGCAGGCG
GGGCCTGGCCCGCCGTGACCAGGCGGTACAGACCGGCTTCATCAGCCCGATCCGGCCCCTGGGGCTGCCC
CTGAGCTCCCGGCCGGCCGCCGTGTGCCCCCCATCAGCGCGGGTGCTGGGCTCGCCCTCCCGCTCGCCAG
CTGGACCCTTAGCATCCTCTGCCGCCTGCCACACATCATCCTCCACTTCCACATCCACTGCCTTCTCCTC
GTCGACCCGCTTCATGCCTGGCACCATCTGGTCCTTCTCTCACGCCCGCCGGCTTGGACCAGGACTGGAA
CCCACGCTGGTGCAAGGGCCTGGCCTGTCGTGGGTACACCCTGACGGGGTGGGCGTCCAGATCGACACCA
TCACCCCTGAGATCCGTGCCCTGTACAACGTGCTGGCCAAAGTGAAGCGGGAGCGGGACGAGTACAAGCG
AAGGTGGGAAGAGGAATACACGGTTCGGATACAGCTACAAGAGCGAGTGACTGAGCTCCAGGAGGAGGCC
CAAGAGGCCGATGCCTGCCAAGAGGAGCTAGCCATGAAGGTTGAGCAGCTGAAGGCAGAGCTGGTGGTCT
TCAAGGGGCTCATGAGTAATAATCTGACAGAGCTGGACACCAAGATCCAGGAAAAGGCCATGAAAGTCGA
CATGGACATCTGCCGCCGCATTGACATCACGGCCAAGCTCTGTGACTTGGCCCAGCAGCGCAACTGTGAG
GATATGATCCAGATGTTCCAGAAGCTGGTCCCGTCCATGGGGGGGCGGAAGCGGGAGCGCAAGGCTGCTG
TGGAGGAGGACACCTCCCTGTCGGAGAGTGATGGGCCCCGCCAGCCTGAGGGTGCCGAGGAGGAGAGCAC
AGCCCTCAGCATCAACGAGGAGATGCAGCGCATGCTCAGCCAGCTGAGGGAGTATGATTTTGAGGACGAC
TGTGACAGCCTGACTTGGGAGGAGACTGAGGAGACCTTGCTACTTTGGGAGGATTTCTCAGGCTATGCCA
TGGCAGCCGCAGAGGCCCAGGGAGAGCAGCAGGAGGACAGCCTGGAGAAGGTGATCAAAGACACGGAGTC
CCTGTTCAAAACCCGGGAGAAGGAGTACCAGGAGACCATTGACCAGATAGAGCTGGAGCTGGCCACAGCC
AAGAACGACATGAACCGACACCTGCATGAGTACATGGAGATGTGCAGCATGAAGCGGGGCCTGGACGTGC
AGATGGAGACCTGCCGCCGGCTCATCACACAGTCCGGGGACCGAAAGTCTCCTGCTTTCACTGCGGTCCC
GCTTAGCGACCCGCCGCCACCGCCGAGTGAGACTGAGGACTCCGATCGAGACGTCTCATCTGATAGCTCC
ATGAGATAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001302778
Insert Size 1689 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001302778.1, NP_001289707.1
RefSeq Size 2866 bp
RefSeq ORF 1689 bp
Locus ID 320678
UniProt ID Q8BXL9
Gene Summary Nuclear matrix protein involved in the immobilization of broken DNA ends and the suppression of chromosome translocation during DNA double-strand breaks (DSBs) (PubMed:31548606). Interacts with the nuclear lamina component LMNA, resulting in the formation of a nucleoskeleton that will relocalize to the DSB sites in a XRCC4-dependent manner and promote the immobilization of the broken ends, thereby preventing chromosome translocation (PubMed:31548606). Acts as a scaffold that allows the DNA repair protein XRCC4 and LMNA to assemble into a complex at the DSB sites (PubMed:31548606).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (3) uses two alternate splice sites in the coding region but maintains the reading frame compared to variant 1. It encodes isoform c, which is the same length as isoform a.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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