Iffo1 (NM_001302778) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4733401N06Rik; A930037G23Rik; HOM-TES-103; Iffo
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228315 representing NM_001302778 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATCCGTTATTTGGTCCCAACCTCTTCCTCCTACAGCAGGAGCAGCAAGGCTTGGCCGGGCCGCTGG GGGACCCTCTGGGAGGTGACCACTTTGCCGGGGGAGGGGACTTAGCCTCGGCTCCGCTTGCCTCGGCCGG CCCCTCTGCCTATTCGCCTCCGGGGCCTGGTCCGGCGCCCCCCGCAGCCATGGCTCTCCGCAACGACCTA GGCTCCAACATCAACGTACTGAAGACCCTGAACCTCCGGTTTCGCTGCTTCCTAGCCAAGGTGCACGAGC TAGAGCGCCGAAATCGGCTGCTGGAGAAACAGCTGCAGCAGGCTCTAGAGGAGGGTAAGCAGGGCAGGCG GGGCCTGGCCCGCCGTGACCAGGCGGTACAGACCGGCTTCATCAGCCCGATCCGGCCCCTGGGGCTGCCC CTGAGCTCCCGGCCGGCCGCCGTGTGCCCCCCATCAGCGCGGGTGCTGGGCTCGCCCTCCCGCTCGCCAG CTGGACCCTTAGCATCCTCTGCCGCCTGCCACACATCATCCTCCACTTCCACATCCACTGCCTTCTCCTC GTCGACCCGCTTCATGCCTGGCACCATCTGGTCCTTCTCTCACGCCCGCCGGCTTGGACCAGGACTGGAA CCCACGCTGGTGCAAGGGCCTGGCCTGTCGTGGGTACACCCTGACGGGGTGGGCGTCCAGATCGACACCA TCACCCCTGAGATCCGTGCCCTGTACAACGTGCTGGCCAAAGTGAAGCGGGAGCGGGACGAGTACAAGCG AAGGTGGGAAGAGGAATACACGGTTCGGATACAGCTACAAGAGCGAGTGACTGAGCTCCAGGAGGAGGCC CAAGAGGCCGATGCCTGCCAAGAGGAGCTAGCCATGAAGGTTGAGCAGCTGAAGGCAGAGCTGGTGGTCT TCAAGGGGCTCATGAGTAATAATCTGACAGAGCTGGACACCAAGATCCAGGAAAAGGCCATGAAAGTCGA CATGGACATCTGCCGCCGCATTGACATCACGGCCAAGCTCTGTGACTTGGCCCAGCAGCGCAACTGTGAG GATATGATCCAGATGTTCCAGAAGCTGGTCCCGTCCATGGGGGGGCGGAAGCGGGAGCGCAAGGCTGCTG TGGAGGAGGACACCTCCCTGTCGGAGAGTGATGGGCCCCGCCAGCCTGAGGGTGCCGAGGAGGAGAGCAC AGCCCTCAGCATCAACGAGGAGATGCAGCGCATGCTCAGCCAGCTGAGGGAGTATGATTTTGAGGACGAC TGTGACAGCCTGACTTGGGAGGAGACTGAGGAGACCTTGCTACTTTGGGAGGATTTCTCAGGCTATGCCA TGGCAGCCGCAGAGGCCCAGGGAGAGCAGCAGGAGGACAGCCTGGAGAAGGTGATCAAAGACACGGAGTC CCTGTTCAAAACCCGGGAGAAGGAGTACCAGGAGACCATTGACCAGATAGAGCTGGAGCTGGCCACAGCC AAGAACGACATGAACCGACACCTGCATGAGTACATGGAGATGTGCAGCATGAAGCGGGGCCTGGACGTGC AGATGGAGACCTGCCGCCGGCTCATCACACAGTCCGGGGACCGAAAGTCTCCTGCTTTCACTGCGGTCCC GCTTAGCGACCCGCCGCCACCGCCGAGTGAGACTGAGGACTCCGATCGAGACGTCTCATCTGATAGCTCC ATGAGATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001302778
Insert Size	1689 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001302778.1, NP_001289707.1
RefSeq Size	2866 bp
RefSeq ORF	1689 bp
Locus ID	320678
UniProt ID	Q8BXL9
Gene Summary	Nuclear matrix protein involved in the immobilization of broken DNA ends and the suppression of chromosome translocation during DNA double-strand breaks (DSBs) (PubMed:31548606). Interacts with the nuclear lamina component LMNA, resulting in the formation of a nucleoskeleton that will relocalize to the DSB sites in a XRCC4-dependent manner and promote the immobilization of the broken ends, thereby preventing chromosome translocation (PubMed:31548606). Acts as a scaffold that allows the DNA repair protein XRCC4 and LMNA to assemble into a complex at the DSB sites (PubMed:31548606).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) uses two alternate splice sites in the coding region but maintains the reading frame compared to variant 1. It encodes isoform c, which is the same length as isoform a.

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