Brap (NM_001289544) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	3010002G07Rik; BRAP2; IMP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228312 representing NM_001289544 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGATGAGGAGATCAAAAAGAAGACATTGGCTTCCGCTGTCGCCTGTCTAGAAGGGAAGTCAGCCG GGGAGAAAGCAGCCATCATCCACCAGCACCTTGGCCGTCGGGAAATGACGGATGTGATTATTGAGACCAT GAAGGCCAGAGCAGATGAAGTGAGAGACACAGTGGAAGAAAAGAAGCCTTCAGCAGCCCCTGTCTCTGCA CAGAGAAGTAGAGAACAGAGTGAGTCTGTGAACACAGCCCCAGAGTCTCCATCCAAACAGCTCCCAGACC AGATTTCCTTCTTCAGTGGCAACCCTTCTGTTGAAATAGTCCACGGCATTATGCACCTGTACAAGACAAA TAAGATGACCTCCCTAAAAGAAGACGTTCGGCGCAGTGCCATGCTGTGTGTCCTCACCGTCCCTGCCACC ATGACCAGTCATGACCTTATGAAGTTTGTTGCCCCATTCAATGATGTAATTGAACAAATGAAAATCATCA GGGACTCTACTCCGAATCAGTACATGGTACTGATCAAGTTCAGTGCGCAGGCTGATGCAGACAGTTTCTA CATGGCGTGCAATGGCCGCCAGTTCAACTCAATCGAAGATGACGTCTGCCAGCTGGTCTATGTGGAAAGG GCTGAAGTGCTGAAATCTGAAGATGGCGCCAGCCTCCCCGTGATGGACCTGACGGAGCTGCCCAAGTGCA CTGTGTGTCTGGAGCGGATGGACGAGTCTGTGAATGGCATCCTCACCACCCTCTGCAACCACAGCTTCCA TAGTCAGTGTCTGCAGCGGTGGGATGACACCACGTGTCCTGTGTGCCGATACTGTCAAACGCCAGAGCCA GTGGAAGAAAACAAATGTTTTGAGTGTGGTGTCCAGGAAAACCTCTGGATTTGTTTAATATGCGGCCACA TAGGCTGTGGGCGGTACGTGAGTCGGCATGCTTACAAGCACTTTGAGGAGACCCAGCACACATACGCCAT GCAGCTCACCAACCATCGAGTCTGGGACTATGCTGGAGATAATTATGTCCATCGACTGGTTGCAAGCAAG ACGGATGGAAAGATCGTTCAGTACGAGTGTGAGGGCGACACCTGCCAGGAAGAGAAGATAGATGCCTTAC AGTTAGAGTACTCGTACCTGTTGACAAGCCAGCTGGAATCGCAGCGGATATACTGGGAGAACAAAATCGT GCGCATAGAGAAGGACACGGCAGAGGAGATTAACAACATGAAGACCAAGTTTAAAGAGACCATCGAGAAG TGTGACAGCCTGGAGCTCAGGCTCAGTGACCTCCTGAAGGAGAAGCAGTCTGTGGAAAGGAAGTGTACCC AGCTGAACACCAGAGTGGCCAAGCTCAGCACGGAGCTGCAGGAGGAGCAGGAGCTGAACAAGTGTCTGCG CGCCAACCAGCTGGTGCTGCAGAACCAGCTCAAGGAGGAGGAGAAGCTGCTGAAGGAGACCTGTGCCCAG AAAGACCTGCAGATCACCGAGATCCAGGAGCAGCTGCGCGATGTCATGTTCTACCTGGAGACACAGCAGC AGATCAGCCACCTGCCTGCGGAGACGAGGCAGGAGATCCAGGAAGGCCAGATCAACATCGCCATGGCCTC AGCGCCCAACCCACCCTCTTCCGGGGCCGGTGGGAAGCTGCAGTCCAGAAAGGGCCGCAGCAAGAGGGGC AAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289544
Insert Size	1686 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289544.1, NP_001276473.1
RefSeq Size	3865 bp
RefSeq ORF	1686 bp
Locus ID	72399
UniProt ID	Q99MP8
Gene Summary	Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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