Asic1 (NM_001289791) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Accn2; AI843610; ASIC; ASIC1a; B530003N02Rik; BNaC2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228306 representing NM_001289791 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCATCCAGATCTTTTGCTCTGTGTCATTCTCCTCTGGAGAGGAGGCCCCAGGATCCATGGGAGATA TCTGGGGTCCCCACCACCACCACCGGCAGCAGCAGGACAGCTCAGAATCGGAAGAAGAAGAAGAGAAGGA AAAGGAGTCAGGGATGGAGCTGGATGAGGGTGACTCACCTAGGGACTTGGTGGCCTTTGCCAACAGTTGT ACCCTCCACGGTGCCAGCCATGTCTTTGTGGAAGGGGGCCCAGGGCCACGGCAGGCCCTATGGGCGGTGG CCTTTGTCATAGCACTGGGTGCCTTCCTGTGCCAGGTAGGGGACCGAGTTGCTTATTACCTCAGCTACCC GCACGTGACTTTGCTAGATGAAGTGGCCACCACAGAGCTGGTCTTCCCAGCTGTCACCTTCTGTAACACC AATGCCGTGCGGTTGTCCCAGCTCAGCTACCCTGACTTGCTCTACCTGGCCCCCATGCTCGGGTTGGATG AGAGTGACGATCCTGGGGTGCCCCTTGCTCCACCTGGGCCAGAGGCTTTCTCGGGCGAGCCCTTTAATCT CCATCGTTTCTACAACCGCTCTTGCCACCGCCTGGAGGACATGCTGCTCTATTGCTCCTACTGTGGGGGC CCCTGTGGTCCCCACAACTTCTCAGTGGTCTTCACGCGGTATGGGAAGTGCTACACATTCAACTCGGGCC AAGATGGGCGGCCACGGCTGAAGACCATGAAAGGTGGGACTGGCAACGGCCTGGAGATCATGCTGGACAT TCAGCAAGATGAATACTTGCCTGTGTGGGGAGAGACTGATGAGACATCGTTCGAAGCAGGCATCAAAGTG CAGATCCACAGTCAGGACGAGCCTCCTTTCATCGACCAGCTGGGCTTTGGCGTGGCCCCAGGCTTCCAGA CGTTTGTGTCTTGCCAGGAGCAGAGGCTCATCTACCTGCCCTCCCCCTGGGGCACCTGCAATGCTGTTAC CATGGACTCGGATTTCTTCGACTCCTACAGCATCACGGCCTGCCGGATTGATTGTGAAACCCGTTACCTG GTGGAAAACTGCAACTGCCGTATGGTGCACATGCCAGGGGATGCCCCATACTGTACTCCGGAGCAGTACA AGGAGTGTGCAGACCCTGCCCTGGACTTCCTAGTGGAGAAAGACCAGGAATACTGTGTGTGTGAGATGCC CTGCAACCTGACCCGCTACGGCAAGGAGCTGTCCATGGTCAAGATCCCCAGCAAAGCCTCAGCCAAGTAC CTGGCCAAGAAGTTCAACAAATCTGAACAGTACATAGGGGAGAATATTCTGGTGCTGGACATTTTCTTTG AAGTCCTCAACTATGAGACCATCGAGCAGAAGAAGGCCTATGAGATCGCAGGGCTTTTGGGTGACATCGG GGGCCAGATGGGATTGTTCATCGGGGCCAGCATCCTCACAGTGCTGGAACTCTTTGACTATGCCTATGAG GTCATTAAGCACCGGCTGTGTAGACGTGGGAAGTGCCAGAAGGAGGCTAAGAGGAACAGCGCAGATAAGG GCGTGGCGCTCAGCCTGGATGACGTCAAAAGACACAATCCCTGCGAGAGCCTCCGAGGACATCCTGCCGG GATGACGTACGCTGCCAACATCCTACCTCACCATCCCGCTCGAGGCACGTTTGAGGACTTTACCTGCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289791
Insert Size	1680 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289791.1, NP_001276720.1
RefSeq Size	4288 bp
RefSeq ORF	1680 bp
Locus ID	11419
UniProt ID	Q6NXK8
Gene Summary	Proton-gated sodium channel; it is activated by a drop of the extracellular pH and then becomes rapidly desensitized. Generates a biphasic current with a fast inactivating and a slow sustained phase. Has high selectivity for sodium ions and can also transport lithium ions with high efficiency. Can also transport potassium ions, but with lower efficiency. It is nearly impermeable to the larger rubidium and cesium ions. Mediates glutamate-independent Ca(2+) entry into neurons upon acidosis. This Ca(2+) overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca(2+) concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR and represents use of an alternate promoter, compared to variant 1. It encodes isoform 2 which has a distinct N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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