Ecm1 (NM_001252653) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI663821; p85
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228302 representing NM_001252653 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGACCGTATCCAGAGCAGCCTTGATCTTGGCCTGCTTGGCTCTTGCTTCTGCTGCCTCTGAGGGAG CCTTCAAGGCTTCAGACCAGCGAGAGATGACGCCAGAGCGCCTCTTCCAGCACCTCCATGAAGTAGGTTA TGCAGCACCCCCTTCCCCACCACAAACCCGGAGACTCCGAGTTGACCACTCTGTAACTTCTCTGCATGAC CCTCCCCTCTTTGAGGAACAAAGAGAAGTGCAGCCCCCTTCCTCTCCAGAAGACATCCCTGTGTACGAGG AAGACTGGCCCACTTTCCTAAACCCTAATGTAGATAAAGCTGGTCCTGCTGTCCCTCAAGAAGCCATCCC CCTGCAGAAAGAGCAGCCCCCTCCCCAAGTCCATATTGAACAGAAGGAAAACCCGCCTGCCCAGCCTCAG GAGGAGATTGTCCAGAAAGAGGTGAAGCCACACACCTTGGCGGGCCAGCTCCCTCCAGAGCCCCGGACTT GGAATCCAGCCCGTCACTGCCAGCAGGGACGGAGAGGTGTCTGGGGCCACCGGCTGGATGGCTTCCCTCC TGGACGGCCTTCTCCAGACAATCTGAAGCAGATCTGCCTTCCTGAGCGTCAGCATGTGATCTACGGCCCC TGGAACCTGCCGCAGACTGGCTACTCTCACCTTAGTCGCCAGGGAGAGACCCTCAATGTGCTGGAGACCG GATACTCCCGCTGCTGTCGCTGCCGCAGCGACACAAACCGCCTAGACTGTTTGAAGCTTGTGTGGGAGGA TGCAATGACCCAATTTTGTGAGGCCGAATTCTCTGTCAAGACCCGCCCCCACCTGTGCTGCAGACTGCGT GGGGAGGAGCGATTCTCTTGCTTCCAGAAGGAAGCTCCTCGCCCAGACTACCTGCTCCGACCCTGCCCCG TCCACCAGAATGGCATGTCCTCAGGGCCCCAGTTGCCTTTCCCCCCGGGGTTGCCCACACCGGACAATGT CAAAAACATCTGTCTCCTGAGACGCTTCCGCGCCGTGCCACGCAACCTCCCAGCTACTGACGCCATCCAG AGGCAGCTGCAGGCTCTGACTCGGCTGGAGACGGAGTTCCAGCGCTGCTGCCGCCAGGGCCACAACCACA CTTGCACATGGAAGGCCTGGGAGGGTACCCTGGATGGATACTGCGAGCGGGAGCTGGCTATAAAGACCCA CCCCCACTCGTGCTGCCACTACCCTCCTAGTCCTGCCCGTGATGAGTGCTTCGCCCACCTAGCTCCCTAT CCCAACTATGACCGGGATATCTTGACCCTTGACCTCAGCCGAGTCACCCCCAACCTCATGGGCCAGCTCT GTGGAAGTGGAAGGGTCCTTAGCAAGCATAAACAGATTCCGGGGCTGATCCAGAATATGACCATCCGCTG CTGCGAGCTTCCATATCCAGAACAGGCCTGCTGCGGCGAAGAGGAGAAACTGGCCTTCATCGAGAACCTC TGTGGTCCCCGGAGGAATTCGTGGAAAGACCCTGCCCTCTGCTGTGACCTGTCTCCTGAAGATAAGCAAA TCAACTGCTTCAATACCAACTACCTGAGGAACGTGGCTTTAGTGGCTGGAGACACTGGGAATGCCACTGG CTTGGGGGAGCAGGGCCCAACTCGGGGAACAGATGCCAACCCCGCCCCTGGGTCCAAGGAAGAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252653
Insert Size	1677 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252653.1, NP_001239582.1
RefSeq Size	1897 bp
RefSeq ORF	1677 bp
Locus ID	13601
Gene Summary	Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity (By similarity).[UniProtKB/Swiss-Prot Function]

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