Mier1 (NM_001286221) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4933425I22Rik; 5830411K19Rik; er1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228290 representing NM_001286221 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGCATCAGATGTTTATGTTTAATTGGTTTACAGACTGTCTCTGGATTCTTTTCCTGTCAAATTACAA GCCATCTGTTGAGTCTTCAAGTCCAGGGTGAATATAAGTGTGGCCTGCTCTGTGAAAACACTTCATTGTT GATTGCTAATGTCAGAAGAAAAGTTTCAGGAGGCTCAGCAACATCAGAGGACCATGAGTTTGATCCATCA GCTGACATGCTGGTTCATGATTTTGATGATGAGCGAACATTAGAAGAAGAAGAAATGATGGAAGGAGAGA CAAACTTCAGTTCTGAAATAGAGGATCTTGCGAGGGAAGGCGACATGCCAATCCACGAGCTGCTCAGCCT CTATGGTTATGACAGCACTGTTCGGTTACCTGAGGAAGAGGAGGAGGAAGAGGAGGAGGAGGAAGGTGAA GATGATGAAGATGCTGATAATGATGATAACAGTGGCTGTAGTGGAGAAAATAAAGAAGAGAATATAAAGG ATTCATCGGGTCAGGAGGATGAAACTCAGTCTTCCAATGATGATCCCTCACAGTCTGTTACTTCCCAAGA TGCTCAGGAAATAATCCGCCCACGTCGATGTAAATATTTTGATACAAATAGTGAAATAGAAGAAGAATCT GAAGAAGATGAAGATTATATTCCCTCAGAAGACTGGAAAAAGGAAATCATGGTGGGCTCCATGTTTCAAG CTGAGATTCCAGTTGGTGTTTGTAGATACAAAGAAAATGAAAAAGTGTATGAAAATGATGATCAGCTTCT GTGGGATCCAGAGTGTTTACCAGAAGAGAAAGTGGTTGTCTTTCTTAAGGATGCGTCTAGAAGGACAGGG GATGAGAAAGGTGTGGAAGCAATCCCTGAAGGCTCGCATATCAAAGACAATGAGCAGGCTTTATATGAGT TGGTTAAATGCAGCTTTGATACGGAAGAAGCCTTGAGAAGACTGAGATTTAATGTCAAAGCAGCTCGAGA GGAGTTATCTGTGTGGACAGAGGAAGAGTGTAGAAATTTTGAGCAAGGGCTGAAGGCCTATGGAAAAGAT TTTCATCTGATTCAGGCTAATAAAGTCCGAACAAGATCAGTTGGTGAATGTGTAGCATTCTATTACATGT GGAAAAAGTCTGAGCGCTATGATTTCTTTGCTCAGCAAACAAGGTTTGGAAAAAAGAAATATAATCTTCA TCCTGGTGTAACGGATTACATGGATCGTCTTTTGGATGAAAGTGAAAGTGCTGCTTCTAGCCGAGCACCA TCCCCCCCTCCCACTGCCTCAAATAGCAGTAACAGCCAGTCCGAGAAAGAAGATGGCGCTGTCAGCAGTA GGAATCAGAACGGTGTCTCATCTAATGGACCAGGAGAAATACTAAACAAAGAAGAAGTAAAAGTTGAAGG GTTACATGTTAATGGACCAACAGGTGGAAATAAGAAACCACTTCTTACAGATATGGACACTAATGGTTAT GAAGCAAATAACCTGACCACTGACCCAAAACTTGCCCATATGACTGCAAGAAATGAAAATGATTTTGATG AAAAAAATGAGAGACCTGCCAAAAGGCGGCGGATAAACAGCAGTGGGAAAGAAAGTCCGGGCTCATCTGA GTTTTTCCAGGAAGCAGTCTCACATGGGAAGTTTGAGGAACATGAAAACACGAATGACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286221
Insert Size	1671 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286221.1, NP_001273150.1
RefSeq Size	4781 bp
RefSeq ORF	1671 bp
Locus ID	71148
UniProt ID	Q5UAK0
Gene Summary	Transcriptional repressor regulating the expression of a number of genes including SP1 target genes. Probably functions through recruitment of HDAC1 a histone deacetylase involved in chromatin silencing.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) encodes the longest isoform (c). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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