Zkscan6 (NM_001302375) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1700128E15Rik; D11Ertd714e; KOX11; Zfp535; Znf18
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228285 representing NM_001302375 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGTTGACTTGGGGCAGGCCCTGGGCCCGCTTCCTTTTCTGGCCAAGGCTGAGGATGCCACATTCT CTGCATCAGATGCTACCCAGCAAAGAGAGCTTGCCAACCCTGAGACTGCTCGCCAGCTTTTCAGGCAGTT TCGTTACCAGGTGCTATCGGGGCCCCAGGAGACCCTGAGACAACTCCGGAAGCTCTGTTTCCAGTGGCTG CGGCCAGAGGTTCACACCAAAGAGCAGATCCTGGAGCTGCTCATGCTGGAGCAGTTTCTGACCATCCTCC CCGGGGAGATCCAGATGTGGGTGAGGAAGCAGTGTCCAGGCAGTGGACAGGAGGCAGTGACGCTCGTGGA AAGCTTGAAGGGAGAACCCCAGAAACTGTGGCACTGGATCAGCACCCAAGTTCTAGGACAAGAGATTCCA TTCGAGAAGGAGAATTTGACACACTGCCCTGGGGACAAGTTGGAGCCTGCACTGGAGGTGGAGCCCTCAC TGGAAGTGGCACCTCAGGACCTGCCCCTTCAGAATTCATCCTCAGCAACTGGGGAGCTACTGAGCCATGG TGTGAAAGAGGAATCAGACATGGAGCCAGAATTAGCTCTGGCTGCTTCCCAGCTTCCTGCCAGACCTGAG GAGAGGCCTGTTAGAGACCAAGAGTTGGGAACAGCAGTTCTCCCACCACTACAGGAGGAGCAGTGGAGAC ACCTGGATTCTACTCAGAAGGAACAGTACTGGGATCTCATGCTGGAGACCTATGGGAAAATGGTCTCAGG AGCAGGCATTTCCAACTCCAAACCTGACTTGACTAACTTAGCAGAATATGGGGAAGAGCTGGTGGGCCTG CACCTTCACGGCGCTGAGAAGATGGCAAGACTCCCCTGCAAAGAAGACAGACAAGAAAATGACAAAGAGA ACCTAAACTTGGAAAATCACAGGGACCAGGGATGTCTGGATGTTTTCTGTCAGGCATCAGGTGAGGCTCC ACCACAGACTGCCTTGAGTGATTTCTTCGGTGAGAGTGAGCCGCATCGCTTTGGAGGAGACAGTGTCCCA GAGGCTCTGGAAAACCATCAGGGTGAGGGAACTGGGGCGCATCTCTTTCCTTATGAAAGGGGCTCTGGGA AACAGCCAGGTCAGCACATACAGAGCTCGTCTTTGGGAGAACTGACTGCCTTGTGGCTAGAGGAGAAGAG AGAGGCCTCTCAGAAGGGACAGGCCAGATCCCCCATGGCCCAGAAACTGCCCACTTGCAGAGAGTGTGGG AAAACCTTCTATAGGAATTCACAGCTTGTTTTTCACCAAAGGACTCACACGGGAGAGACATACTTTCATT GCCACATTTGCAAAAAAGCCTTTCTACGAAGCTCAGACTTTGTGAAACATCAGAGAACTCACACAGGAGA GAAGCCCTGTAAATGTGATTACTGTGGGAAAGGCTTCAGTGACTTCTCAGGCTTGCGCCACCACGAGAAA ATCCACACAGGAGAGAAACCCTATAAATGCCCCCTCTGTGAGAAAAGCTTTATTCAGAGGTCAAACTTCA ATAGACATCAAAGGGTTCATACGGGTGAGAAACCTTATAAATGTACCCACTGTGGCAAGCAGTTCAGCTG GAGCTCCAGCCTTGACAAGCATCAGAGATCACACTTGGGGAAGATGCCCTGCCCATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001302375
Insert Size	1668 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001302375.1, NP_001289304.1
RefSeq Size	2278 bp
RefSeq ORF	1668 bp
Locus ID	52712
UniProt ID	Q810A1
Gene Summary	May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR and uses an alternate splice site in the central coding region compared to variant 1. The encoded isoform (2) is shorter compared to isoform 1.

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