Slc33a1 (NM_001272035) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Acatn; AI315656; AI788741; D630022N01Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228261 representing NM_001272035 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCACCGACCATCTCCCACAAGGACAGCAGTCGGCAGCGGCGGTCAGGCATGTTTAGCCACGCTCTGG ATATGAAGAGTGGCCCGCTGCCACCGGGCGGCTGGGATGACAGTCGCCGAGACTCGGTGGGCGGGGAGGG GGACCGGGAAGTTCTTCTAGGTGATGCCGGCCCTGGTGACTTACCTAAAGCCCCGCGGAGCTATAGATCC GAACTAAGTAGCATTTTGTTACTACTCTTTCTTTACGTGCTTCAGGGCATTCCCTTAGGCCTGGCGGGGA GCATCCCCCTCATTTTGCAAAGCAAAAATGTTAGCTATACAGATCAGGCTTTCTTCAGTTTTGTCTTCTG GCCATTCAGTCTTAAGTTGCTCTGGGCTCCCTTGGTTGATGCCGTCTACTTTAAGAACTTTGGTCGTCGC AAGTCCTGGCTTGTCCCTACTCAGTATACACTAGGAATCTTCATGATATATTTATCCACTCAAGTGGACC GTTTGCTCGGAAATATTGATGGCAGAACACCGGATGTAGTTGCTCTCACTGTGACATTCTTCTTGTTTGA ATTCCTGGCTGCCACACAGGACATCGCTGTGGATGGTTGGGCATTAACTATGTTATCCCGGGAAAACGTG GGCTATGCTTCGACATGCAATTCTGTGGGCCAAACAGCTGGCTACTTTTTGGGCAATGTTTTATTTTTGG CTCTTGAATCTGCTGACTTTTGTAACAAATACTTGCGGTTTCAGCCTCAACCCAGGGGAATCGTTACCCT TTCAGATTTTCTATTTTTCTGGGGAACTGTATTTTTAATAACAACAACATTAGTAGCCCTTCTGAAAAAA GAAAACAGAGAAGCGTCAATAGTAAAAGAAGAGACCCAAGGGATCACAGACACTTACAAGCTGCTATTCT CAATTATAAAAATGCCAGCAGTTCTGGCCTTTTGCCTTCTGATTCTAACGTCAAAGATTGGCTTCTCAGC AGCTGATGCTGTGACAGGCCTGAAGCTGGTGGAAGAAGGGGTGCCTAAAGAGCACCTGGCCTTACTAGCT GTCCCAATGGTCCCTCTGCAGATAATCCTGCCACTCCTCATCAGCAAGTATACTGCAGGTCCCCAGCCTC TGAACATATTTTACAAAGCCATGCCCTACAGATTATTGCTTGGATTAGAATATGCTCTACTTGTTTGGTG GACTCCTAAAGTAGAGCATCAAGGAGGATTCCCTCTATATTACTATATTATAGTGCTGCTGAGTTATGCA TTACATCAGGTCACTCTGTACAGCATGTATGTGTCGATAATGGCTTTCAATGCCAAGGTCAGTGATCCTC TTATTGGGGGAACATACATGACCCTTTTAAACACTGTGTCTAATCTGGGAGGAAACTGGCCTTCTACAGT GGCTCTTTGGCTAGTGGATCCCCTCACAGTGAAGGAATGTGTAGGAGCTTCAAACCAGAATTGTCGGACA CCTGATGCTATCGAACTTTGCAAAAAACTTGGAGGCTCGTGTGTTACAGCTCTGGATGGTTACTATGTGG AATCCATTATCTGTGTTCTCATTGGATTTGGTTGGTGGTTCTTTCTTGGTCCAAAATTTAAAAAGTTACA GGATGAAGGACCATCCTCATGGAAGTGCAAAAGGAACAACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001272035
Insert Size	1653 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001272035.1, NP_001258964.1
RefSeq Size	2430 bp
RefSeq ORF	1653 bp
Locus ID	11416
UniProt ID	Q99J27
Gene Summary	Probable acetyl-CoA transporter necessary for O-acetylation of gangliosides (PubMed:10570973). Negatively regulates BMP signaling (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 3' UTR compared to variant 1. Both variants 1 and 2 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data.

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