Pip5k1a (NM_001293707) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	PI4P5K-I[a]; PIP5K1-alpha; PIP5KIalpha; Pipk5a
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228253 representing NM_001293707 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCCGCCTCCTCAGGGCCAGCGGCGGCCGGGTTTTCATCCCTTGATGCCGGGGCCCCTGCTGGTA CCGCAGCAGCAGCATCTGGAATCAAGAGAGCCACAGTATCTGAGGGACCTTCCGCCTCTGTCATGCCTGT TAAAAAAATAGGCCATCGAAGTGTTGATTCCTCTGGAGAGACTACATACAAGAAGACAACTTCATCAGCC TTGAAAGGTGCCATCCAGTTAGGCATCACTCACACTGTGGGCAGCCTGAGCACCAAACCAGAGCGTGATG TCCTCATGCAAGACTTCTACGTGGTGGAGAGCATCTTTTTCCCCAGTGAAGGCAGCAACCTGACACCGGC TCACCATTACAATGACTTTCGATTCAAGACCTATGCGCCTGTTGCCTTCCGTTACTTCAGAGAGCTCTTT GGCATCCGGCCTGATGATTACTTGTACTCCCTTTGCAGTGAGCCATTGATTGAACTCTCCAATTCTGGAG CTAGTGGTTCCCTCTTCTATGTGTCCAGTGATGATGAATTCATCATTAAGACCGTCCAGCATAAAGAAGC AGAATTTCTGCAGAAGTTGCTTCCAGGATACTACATGAATCTTAACCAAAACCCTCGTACTTTGCTGCCC AAATTTTATGGATTGTACTGTGTGCAAGCAGGCGGCAAGAACATACGAATTGTGGTGATGAACAATCTCT TGCCTCGGTCAGTCAAAATGCATATGAAATATGACCTGAAGGGTTCAACTTACAAGCGGCGGGCTTCTCA GAAAGAACGAGAGAAAACTCTCCCCACCTTTAAAGACCTGGACTTCCTACAAGATATCCCTGACGGCCTA TTTTTGGATGCTGACATGTACAGTGCTCTGTGTAAGACTCTACAGCGTGACTGTTTGGTGCTGCAGAGCT TCAAGATAATGGACTACAGCCTGTTGATGTCCATCCACAACATGGACCATGCACAGAGGGAGCCCACGAG CAATGACACACAGTACTCGGCTGACACGCGCAGACCAGCCCCACAGAAGGCGCTCTATTCCACAGCTATG GAATCCATCCAGGGCGAGGCTCGACGGGGCGGCACTGTGGAGACTGAGGACCACATGGGTGGCATCCCTG CCCGGAATAACAAAGGAGAAAGGCTCCTGCTTTATATTGGCATCATTGATATTCTTCAGTCCTACAGGTT TGTTAAGAAGTTGGAGCACTCTTGGAAAGCACTGGTCCATGATGGGGATACGGTGTCAGTGCATCGTCCA GGCTTCTATGCTGAGCGGTTCCAGCGTTTCATGTGCAACACAGTGTTCAAGAAGATTCCCTTGAAGCCTT CTCCTACCAAAAAGTTTCGGTCTGGCCCGTCTTTCTCTCGGCGATCAGGCCCCAGCGGCAACTCCTGCAC CTCCCAGCTGATGGCCTCTGGGGAACACAGAGCACAAGTGACCACCAAGGCGGAAGTGGAGCCAGATGTA CACCTTGGGCGTCCTGATGTCTTACCTCAGACTCCACCTTTGGAGGAAATAAGTGAGGGTTCACCTGTTC CTGGCCCCAGTTTCTCACCTGTAGTTGGACAACCTTTGCAAATACTAAATTTGAGTTCAACCTTGGAAAA GCTTGATGTTGCAGAGTCAGAGTTCACCCACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001293707
Insert Size	1644 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001293707.1, NP_001280636.1
RefSeq Size	3676 bp
RefSeq ORF	1644 bp
Locus ID	18720
UniProt ID	P70182
Gene Summary	Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PtdIns4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). PtdIns(4,5)P2 is involved in a variety of cellular processes and is the substrate to form phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3), another second messenger. The majority of PtdIns(4,5)P2 is thought to occur via type I phosphatidylinositol 4-phosphate 5-kinases given the abundance of PtdIns4P. Participates in a variety of cellular processes such as actin cytoskeleton organization, cell adhesion, migration and phagocytosis. Required for membrane ruffling formation, actin organization and focal adhesion formation during directional cell migration by controlling integrin-induced translocation of RAC1 to the plasma membrane. Together with PIP5K1C is required for phagocytosis, but they regulate different types of actin remodeling at sequential steps. Promotes particle ingestion by activating WAS that induces Arp2/3 dependent actin polymerization at the nascent phagocytic cup. Together with PIP5K1B is required after stimulation of G-protein coupled receptors for stable platelet adhesion. Plays a role during calcium-induced keratinocyte differentiation. Recruited to the plasma membrane by the E-cadherin/beta-catenin complex where it provides the substrate PtdIns(4,5)P2 for the production of PtdIns(3,4,5)P3, diacylglycerol and inositol 1,4,5-trisphosphate that mobilize internal calcium and drive keratinocyte differentiation. Together with PIP5K1C have a role during embryogenesis. Functions also in the nucleus where acts as an activator of TUT1 adenylyltransferase activity in nuclear speckles, thereby regulating mRNA polyadenylation of a select set of mRNAs (PubMed:10679324, PubMed:18772378, PubMed:19153220, PubMed:20622009, PubMed:8798574). Positively regulates insulin-induced translocation of SLC2A4 to the cell membrane in adipocytes (PubMed:27739494).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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