Abcg8 (NM_001286005) Mouse Untagged Clone

CAT#: MC228250

Abcg8 (untagged) - Mouse ATP-binding cassette, sub-family G (WHITE), member 8 (Abcg8), transcript variant 2



  "NM_001286005" in other vectors (1)

CNY 5,230.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 1300003C16Rik; AI114946; sterolin-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC228250 representing NM_001286005
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAATCAGGACAAATTTGGATAAATGGGCAACCCAGTACGCCTCAGCTGGTGAGGAAGTGCGTTGCGC
ATGTGCGGCAGCATGACCAACTGCTGCCCAACCTGACCGTCAGAGAGACCCTGGCTTTCATTGCCCAGAT
GCGCCTGCCCAGGACCTTCTCCCAGGCCCAGCGTGACAAACGGGTGGAAGACGTAATCGCCGAGCTGCGG
CTGCGGCAGTGCGCCAACACCAGAGTGGGCAACACGTATGTACGTGGGGTGTCCGGGGGTGAGCGCCGAC
GAGTGAGCATTGGGGTGCAGCTCCTGTGGAACCCAGGAATCCTCATTCTGGATGAACCCACTTCTGGCCT
CGACAGCTTCACAGCCCACAATCTGGTGACAACCTTGTCCCGCCTGGCCAAGGGCAACAGGCTGGTGCTC
ATCTCCCTCCACCAGCCTCGCTCTGACATCTTCAGGCTATTTGACCTGGTCCTTCTGATGACATCTGGCA
CCCCTATCTACCTGGGGGCGGCGCAGCAAATGGTGCAGTACTTCACATCCATTGGCCACCCTTGTCCTCG
CTATAGCAACCCTGCGGACTTCTACGTGGACTTGACCAGCATCGACAGACGCAGCAAAGAACGGGAGGTG
GCCACCGTGGAGAAGGCACAGTCTCTTGCAGCCCTGTTCCTAGAAAAAGTACAAGGCTTTGATGACTTTC
TGTGGAAAGCTGAGGCAAAGGAACTCAACACAAGCACCCACACAGTCAGCCTGACCCTCACACAGGACAC
TGACTGTGGGACTGCTGTTGAGCTGCCCGGGATGATAGAGCAGTTTTCCACCCTGATCCGTCGTCAGATT
TCCAATGACTTCCGGGACCTGCCCACGCTGCTCATTCATGGGTCGGAAGCCTGCCTGATGTCCCTCATCA
TTGGCTTCCTTTACTACGGCCATGGGGCCAAGCAGCTCTCCTTCATGGACACAGCAGCCCTCCTCTTCAT
GATAGGGGCGCTCATTCCTTTCAATGTCATCCTGGATGTCGTCTCCAAATGTCACTCGGAGAGGTCAATG
CTGTACTATGAGCTGGAAGACGGGCTGTACACTGCTGGTCCTTATTTCTTTGCCAAGATCCTAGGAGAAT
TGCCGGAGCACTGTGCCTACGTCATCATCTACGCGATGCCCATCTACTGGCTGACAAACCTGCGGCCCGT
GCCTGAGCTCTTCCTTCTACACTTCCTGCTCGTGTGGTTGGTGGTCTTCTGCTGCAGGACCATGGCCCTG
GCTGCCTCTGCCATGCTGCCCACCTTCCACATGTCCTCCTTCTTCTGCAATGCCCTCTACAACTCCTTCT
ACCTTACTGCCGGCTTCATGATAAACTTGGACAACCTGTGGATAGTGCCTGCATGGATCTCCAAGCTGTC
GTTCCTCCGGTGGTGCTTCTCGGGGCTGATGCAGATTCAATTTAATGGACACCTTTACACCACACAAATC
GGCAACTTCACCTTCTCCATCCTCGGAGACACGATGATCAGTGCCATGGACCTGAACTCGCATCCACTCT
ATGCGATCTACCTCATTGTCATCGGCATCAGCTACGGCTTCCTGTTCCTGTACTATCTATCCTTGAAGCT
CATCAAACAGAAGTCAATTCAAGACTGGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001286005
Insert Size 1641 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001286005.1, NP_001272934.1
RefSeq Size 3432 bp
RefSeq ORF 1641 bp
Locus ID 67470
UniProt ID Q9DBM0
Gene Summary ABCG5 and ABCG8 form an obligate heterodimer that mediates Mg(2+)- and ATP-dependent sterol transport across the cell membrane (PubMed:16352607, PubMed:16867993, PubMed:18402465). Plays an essential role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile (PubMed:12444248, PubMed:14504269, PubMed:14657202, PubMed:25378657). Plays an important role in preventing the accumulation of dietary plant sterols in the body (PubMed:12444248, PubMed:14657202). Required for normal sterol homeostasis (PubMed:12444248, PubMed:14657202). The heterodimer with ABCG5 has ATPase activity (PubMed:16352607, PubMed:16867993).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) contains a distinct 5' UTR and lacks an in-frame portion of the 5' coding region, compared to variant 1. The resulting isoform (2) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...