Abcg8 (NM_001286005) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1300003C16Rik; AI114946; sterolin-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228250 representing NM_001286005 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAATCAGGACAAATTTGGATAAATGGGCAACCCAGTACGCCTCAGCTGGTGAGGAAGTGCGTTGCGC ATGTGCGGCAGCATGACCAACTGCTGCCCAACCTGACCGTCAGAGAGACCCTGGCTTTCATTGCCCAGAT GCGCCTGCCCAGGACCTTCTCCCAGGCCCAGCGTGACAAACGGGTGGAAGACGTAATCGCCGAGCTGCGG CTGCGGCAGTGCGCCAACACCAGAGTGGGCAACACGTATGTACGTGGGGTGTCCGGGGGTGAGCGCCGAC GAGTGAGCATTGGGGTGCAGCTCCTGTGGAACCCAGGAATCCTCATTCTGGATGAACCCACTTCTGGCCT CGACAGCTTCACAGCCCACAATCTGGTGACAACCTTGTCCCGCCTGGCCAAGGGCAACAGGCTGGTGCTC ATCTCCCTCCACCAGCCTCGCTCTGACATCTTCAGGCTATTTGACCTGGTCCTTCTGATGACATCTGGCA CCCCTATCTACCTGGGGGCGGCGCAGCAAATGGTGCAGTACTTCACATCCATTGGCCACCCTTGTCCTCG CTATAGCAACCCTGCGGACTTCTACGTGGACTTGACCAGCATCGACAGACGCAGCAAAGAACGGGAGGTG GCCACCGTGGAGAAGGCACAGTCTCTTGCAGCCCTGTTCCTAGAAAAAGTACAAGGCTTTGATGACTTTC TGTGGAAAGCTGAGGCAAAGGAACTCAACACAAGCACCCACACAGTCAGCCTGACCCTCACACAGGACAC TGACTGTGGGACTGCTGTTGAGCTGCCCGGGATGATAGAGCAGTTTTCCACCCTGATCCGTCGTCAGATT TCCAATGACTTCCGGGACCTGCCCACGCTGCTCATTCATGGGTCGGAAGCCTGCCTGATGTCCCTCATCA TTGGCTTCCTTTACTACGGCCATGGGGCCAAGCAGCTCTCCTTCATGGACACAGCAGCCCTCCTCTTCAT GATAGGGGCGCTCATTCCTTTCAATGTCATCCTGGATGTCGTCTCCAAATGTCACTCGGAGAGGTCAATG CTGTACTATGAGCTGGAAGACGGGCTGTACACTGCTGGTCCTTATTTCTTTGCCAAGATCCTAGGAGAAT TGCCGGAGCACTGTGCCTACGTCATCATCTACGCGATGCCCATCTACTGGCTGACAAACCTGCGGCCCGT GCCTGAGCTCTTCCTTCTACACTTCCTGCTCGTGTGGTTGGTGGTCTTCTGCTGCAGGACCATGGCCCTG GCTGCCTCTGCCATGCTGCCCACCTTCCACATGTCCTCCTTCTTCTGCAATGCCCTCTACAACTCCTTCT ACCTTACTGCCGGCTTCATGATAAACTTGGACAACCTGTGGATAGTGCCTGCATGGATCTCCAAGCTGTC GTTCCTCCGGTGGTGCTTCTCGGGGCTGATGCAGATTCAATTTAATGGACACCTTTACACCACACAAATC GGCAACTTCACCTTCTCCATCCTCGGAGACACGATGATCAGTGCCATGGACCTGAACTCGCATCCACTCT ATGCGATCTACCTCATTGTCATCGGCATCAGCTACGGCTTCCTGTTCCTGTACTATCTATCCTTGAAGCT CATCAAACAGAAGTCAATTCAAGACTGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286005
Insert Size	1641 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286005.1, NP_001272934.1
RefSeq Size	3432 bp
RefSeq ORF	1641 bp
Locus ID	67470
UniProt ID	Q9DBM0
Gene Summary	ABCG5 and ABCG8 form an obligate heterodimer that mediates Mg(2+)- and ATP-dependent sterol transport across the cell membrane (PubMed:16352607, PubMed:16867993, PubMed:18402465). Plays an essential role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile (PubMed:12444248, PubMed:14504269, PubMed:14657202, PubMed:25378657). Plays an important role in preventing the accumulation of dietary plant sterols in the body (PubMed:12444248, PubMed:14657202). Required for normal sterol homeostasis (PubMed:12444248, PubMed:14657202). The heterodimer with ABCG5 has ATPase activity (PubMed:16352607, PubMed:16867993).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) contains a distinct 5' UTR and lacks an in-frame portion of the 5' coding region, compared to variant 1. The resulting isoform (2) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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