Traf3 (NM_001286122) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI528849; amn; CAP-1; CD40bp; CRAF1; LAP1; T-BAM
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228240 representing NM_001286122 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCAAGCAAAAAGATGGATGCTGCTGGCACACTGCAGCCTAACCCACCCCTAAAGCTGCAGCCTG ATCGCGGCGCAGGGTCCGTGCTCGTGCCGGAGCAAGGAGGCTACAAGGAGAAGTTTGTGAAGACGGTGGA AGACAAGTACAAGTGCGAGAAGTGCCGCCTGGTGCTGTGCAACCCGAAGCAGACGGAGTGTGGCCACCGG TTCTGCGAGAGCTGCATGGCCGCCCTGCTGAGCTCCTCCAGTCCAAAATGCACAGCGTGCCAAGAAAGCA TCATCAAAGACAAGGTGTTTAAGGATAATTGCTGCAAGAGAGAGATTCTGGCCCTTCAGGTCTACTGTCG GAATGAAGGCAGAGGTTGTGCGGAGCAGCTGACTCTGGGACATCTGCTGGTGCACCTAAAAAATGAATGT CAGTTTGAGGAACTTCCCTGTCTGCGTGCCGACTGCAAAGAAAAAGTACTGAGAAAAGACTTGCGGGATC ACGTGGAAAAGGCCTGTAAATACCGCGAGGCCACGTGCAGTCACTGCAAGAGCCAAGTGCCCATGATCAA ACTGCAGAAACATGAAGACACAGATTGTCCCTGTGTGGTGGTATCCTGCCCTCACAAGTGCAGCGTTCAG ACTCTTCTAAGGAGTGAGGGTACAAACCAGCAGATCAAGGCCCATGAGGCCAGCTCCGCGGTACAGCACG TGAACCTGCTGAAGGAGTGGAGCAACTCCCTGGAGAAGAAGGTTTCCCTGCTGCAGAATGAAAGTGTTGA GAAAAACAAGAGCATCCAAAGCCTGCACAACCAGATCTGCAGCTTTGAGATCGAGATTGAGAGGCAGAAG GAGATGCTCCGAAACAACGAGTCCAAGATCCTTCACCTGCAGCGGGTAATCGACAGCCAAGCAGAGAAAC TGAAAGAACTGGACAAGGAGATCCGTCCCTTCCGGCAGAACTGGGAGGAAGCGGACAGCATGAAGAGCAG TGTGGAGTCCCTCCAGAACCGAGTGACTGAGCTGGAGAGCGTAGACAAAAGTGCGGGGCAGGCGGCTCGC AACACAGGCTTGCTGGAGTCCCAGCTGAGCCGGCATGACCAGATGTTGAGTGTTCATGACATCCGCTTGG CCGACATGGACCTGCGGTTCCAGGTCCTCGAGACCGCCAGCTACAACGGGGTGCTGATCTGGAAGATCCG TGACTACAAGCGCCGGAAGCAGGAGGCCGTCATGGGGAAGACCCTGTCTCTCTACAGCCAGCCTTTCTAC ACAGGTTATTTTGGCTATAAGATGTGTGCCAGGGTCTACCTGAATGGGGACGGAATGGGGAAAGGGACAC ACTTGTCGCTGTTTTTTGTCATTATGCGTGGAGAATATGATGCTCTGTTGCCATGGCCGTTCAAGCAGAA AGTGACACTTATGCTGATGGATCAGGGGTCCTCTCGCCGTCATCTGGGAGATGCGTTCAAGCCTGACCCC AACAGCAGCAGCTTCAAGAAACCCACCGGAGAGATGAATATCGCCTCTGGCTGCCCAGTCTTTGTCGCCC AAACTGTTCTAGAGAACGGGACGTATATTAAAGATGATACAATCTTTATTAAGGTCATAGTGGATACCTC GGATCTGCCTGACCCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286122
Insert Size	1629 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286122.1, NP_001273051.1
RefSeq Size	6978 bp
RefSeq ORF	1629 bp
Locus ID	22031
Gene Summary	Regulates pathways leading to the activation of NF-kappa-B and MAP kinases, and plays a central role in the regulation of B-cell survival. Part of signaling pathways leading to the production of cytokines and interferon. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Plays a role in the regulation of antiviral responses. Is an essential constituent of several E3 ubiquitin-protein ligase complexes. May have E3 ubiquitin-protein ligase activity and promote 'Lys-63'-linked ubiquitination of target proteins. Inhibits activation of NF-kappa-B in response to LTBR stimulation. Inhibits TRAF2-mediated activation of NF-kappa-B. Down-regulates proteolytic processing of NFKB2, and thereby inhibits non-canonical activation of NF-kappa-B. Promotes ubiquitination and proteasomal degradation of MAP3K14.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks an internal exon in the 5' UTR and an in-frame exon in the coding region, compared to variant 1. The resulting isoform (b) lacks an internal segment, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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