Treh (NM_001277847) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2210412M19Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228233 representing NM_001277847 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCTGGGAGCTGCACCTGCTGCTTCTGCTGGGGCTGGGACTTAGGTCCCAGGAGGCCCTGCCACCAC CCTGTGAGAGATCTACTGCCATGGAGAGCTCCTGCACCAAGTTCAGATGGCCCAGCTCTACCAAGATGAC AAGCAGTTTGTGGATATGTCACTGGCCACATCTCCAGATGAAGTCCTGCAGAAGTTCAGTGAGCTGGCCA CAGTCCACAACCACAGCATCCCCAAGGAACAGCTTCAGGAATTTGTCCAGAGTCACTTCCAGCCCGTGGG GCAGGAGCTGCAGTCCTGGACCCCTGAGGACTGGAAGGACAGCCCTCAGTTCCTGCAGAAGATCTCGGAT GCTAATCTGCGTGTCTGGGCGGAGGAGCTACACAAGATCTGGAAAAAGCTGGGAAAGAAGGGACTCGTAC TGGGTGATGGAAGGCCTGCTTCTTTCTGAGATGGCCTCAACAGTGAAGGGTATGCTGCAAAACTTTCTGG ATCTGGTGAAGACCTACGGACATATCCCCAACGGTGGACGCATATATTACCTGCAACGGAGCCAGCCCCC ACTCCTGACTCTCATGATGGATCGATATGTAGCTCATACCAAGGATGTCGCCTTCCTTCAGGAGAATATT GGGACTCTAGCCTCTGAACTGGACTTCTGGACTGTGAACAGGACTGTCTCTGTAGTCTCAGGAGGACAAA GCTATGTCTTAAATCGCTACTATGTCCCTTATGGGGGACCCAGGCCAGAGTCCTACAGGAAAGACGCAGA ATTGGCAAACTCTGTGCCAGAAGGGGACCGAGAGACTCTGTGGGCTGAGCTCAAGGCTGGGGCTGAGTCT GGCTGGGACTTCTCTTCACGCTGGCTTGTTGGAGGCCCAGACCCTGATTTGCTCAGCAGCATCCGAACCA GCAAAATGGTACCCGCTGATCTGAACGCGTTCCTGTGCCAAGCAGAGGAACTGATGAGTAACTTCTACTC CAGACTAGGGAACGACACAGAGGCCACAAAGTACAGGAACCTGCGGGCCCAGCGCTTGGCCGCCATGGAA GCTGTCCTGTGGGACGAGCAGAAGGGTGCCTGGTTTGACTATGACTTGGAAAAGGGGAAGAAGAACCTGG AGTTTTATCCCTCCAACCTCTCCCCACTTTGGGCTGGCTGCTTCTCAGACCCTAGTGTTGCTGACAAGGC TCTGAAGTACTTGGAGGACAGCAAGATCTTGACCTACCAATATGGAATCCCAACCTCTCTTCGTAACACA GGCCAGCAGTGGGACTTCCCCAATGCCTGGGCCCCACTGCAGGACCTGGTCATTAGAGGTTTGGCCAAGT CAGCTTCCCCCCGGACTCAGGAGGTGGCTTTCCAGCTGGCCCAGAATTGGATCAAAACCAACTTCAAAGT CTACTCCCAAAAGTCAGCGATGTTTGAGAAGTATGACATCAGCAACGGTGGACATCCAGGTGGAGGAGGG GAGTATGAAGTTCAGGAAGGATTTGGCTGGACAAACGGATTGGCCCTGATGCTTCTGGATCGCTATGGTG ACCAGTTGACTTCAGGGACCCAGTTAGCTTCCCTGGGACCCCACTGCCTAGTGGCTGCCCTTCTTCTCAG TCTTCTGCTACAGTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001277847
Insert Size	1626 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001277847.1, NP_001264776.1
RefSeq Size	1943 bp
RefSeq ORF	1626 bp
Locus ID	58866
Gene Summary	This gene belongs to the alpha-glucosidase family, whose members encode enzymes that carry out hydrolysis of alpha-glucoside bonds of a variety of carbohydrates. The enzyme encoded by this gene uses the disaccharide trehalose as a highly specific substrate and converts it into two glucose molecules. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2013] Transcript Variant: This variant (2) uses an alternate splice site causing a frameshift in the 5' coding region, and lacks an alternate exon in the 5' coding region which corrects the frameshift, compared to variant 1. It encodes isoform 2, which is shorter and contains an alternate internal segment, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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