Ippk (NM_001276399) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1810043M15Rik; InsP6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228221 representing NM_001276399 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCTGTGCGTGCCGCGTGGCCTCACACCGTGCAGCCTCCCCCGGGATGCTCGTCTTACTGTCAGGCA ACGGTGGGCCAGAGGCTGAGCCTGGACCAGGGCCTGGGCAGCACAGGGGAAAGTTCCCAGAGTGCTGTCA GCCCACCGAGTGGAGTTCAGCCTCGAGAAACCACAGAGCTGGTGTTCTGCCTTGCTGCTGCGCTGTCAGG AACGGGGCGCTGCCACAGCGCTGTGTAGTTCTGCGTTTTCTGAAGTTTCCTCCAAATAAGAAGAAGACCT CAGAAGAGATACTTCAGCATCTGCAGAACATAGTAGACTTTGGCAAAAATGTCATGAAAGACTTTTTGGG AGAGAACTATGTTCATTGTGGGGAAGTTGTTCAGCTGCCTTTAGAGTTTGTGAAACAACTCTGTTTAAAG ATACAGTGTGAAAGACCAGAGTCCCGTTGTGACAAGGACCTGGACACATTCAGTGGTTATGCTATGTGCC TTCCCAATTTAACCAGACTTCAGACCTTCCACTTTGCAGAGCACCGGCCGATTCTGTGTGTAGAGATTAA GCCAAAATGTGGGTTTATTCCTTTCTCAAATGATGTTACCCACGAGATGAAGCACAAGGTGTGCCGATAC TGCATGCACCAGCACCTCAAGGTAGCAACTGGAAAGTGGAAGAAAATCAGTAAATACTGTCCCCTCGACC TCTACTCAGGAAATAAGCAGCGCATGCACTTTGCCCTCAGAAGTCTGCTGCAGGAGACACAGAATAATCT TAGGATATTTAAGAATGGAGAGCTGATTTATGGTTGTGGTGATGCCCGGAGCCCTGTGGCTGACTTGAAG GAGCTTGCACACCACCTGAAGCCCTTCTTCTTCCCTTCCAACGGCTTGGCCAGTGGGCCTCACTGCACGA AGGCTGTGATCCGTGAGCTGGTGCATGTTATCACCAGGGTGCTGCTGAGCAGCTCAGAAAAAGCCCGAGC TGGTGCCCTGAGGCTTGGGCTCCAGGGCCCACGAGTCTGTGAAGCCAGTCCTTTCAGCAGGAGCCTACAC AACCAAGGAAAAAACACCTCAGAGCACTCGGGGTTACCGAAGGGCTGTCTTCTGTACAAAACCCTCCAGG TGCAGATGTTGGACCAGCTGGACATCGAAGGCCTCTACCCTCTGTACAAACGGGTTGAGCAATACCTGGA GGAGTTTCCTGAGGAGAGGAAAACATTACAGATAGATGGGCCTTATGATGAAGTATTTTACCAGAAACTG CTTGATCTGTCCACTGAGGATGATGGGACAGTGGCCTTTGCATTAACAAAGGTGCAGCAATACAGAGTAG CCATGACTGCCAAGGACTGTTCCATCATGATCGCACTTTCCCCTTGTCTACAGGGCACCAGCTCTGACCA GAGGCCTGTTATTCCTTCGTCAAGATCCCGGCTGGCCTTCTCTGTGTCTGTATTGGACCTCGACCTCAAG CCCTATGAGAGCATTCCTCATCAGTATAAACTAGACAGCAAGATAGTCAACTATTACTCAAAGACTGTGC ATGCCAAAGATGACACTGTGAGGTCGACTCGGTTCAAGGAACATGAAGATTGCACATTAGTTCTCCATAA GGTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001276399
Insert Size	1617 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001276399.1, NP_001263328.1
RefSeq Size	3015 bp
RefSeq ORF	1617 bp
Locus ID	75678
Gene Summary	Phosphorylates Ins(1,3,4,5,6)P5 at position 2 to form Ins(1,2,3,4,5,6)P6 (InsP6 or phytate). InsP6 is involved in many processes such as mRNA export, non-homologous end-joining, endocytosis, ion channel regulation. It also protects cells from TNF-alpha-induced apoptosis.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate 5' exon and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (b) is longer and has a distinct N-terminus, compared to isoform a.

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