Tyrp1 (NM_001282014) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	b; brown; isa; Oca3; TRP-1; TRP1; Tyrp
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228215 representing NM_001282014 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAATCTTACAACGTCCTCCCCCTAGCCTATATCTCCCTTTTCCTGATGCTGTTTTATCAGGTTTGGG CTCAGTTTCCACGAGAGTGTGCCAATATTGAGGCTCTGAGACGTGGGGTGTGTTGCCCAGACCTGCTCCC TTCCTCTGGACCGGGGACTGACCCTTGTGGCTCATCATCAGGAAGAGGCAGGTGTGTGGCTGTGATTGCA GACTCCCGACCCCACAGCCGCCATTATCCCCACGATGGTAAAGATGACCGAGAAGCCTGGCCTCTGAGGT TCTTTAATAGAACATGTCAGTGCAATGATAATTTCTCAGGACACAACTGTGGGACTTGCCGTCCTGGGTG GAGAGGAGCTGCATGCAACCAGAAAATTCTCACAGTCAGGAGAAATCTTCTAGACTTAAGTCCAGAAGAA AAGAGCCACTTTGTCAGGGCCTTGGATATGGCGAAGCGCACAACTCACCCTCAATTTGTCATTGCCACAA GGAGGTTAGAAGACATACTGGGACCAGATGGCAACACACCACAATTTGAGAACATTTCCGTTTATAACTA CTTTGTTTGGACACACTATTATTCAGTCAAAAAAACCTTCCTCGGGACAGGACAGGAAAGCTTTGGGGAT GTGGATTTCTCTCACGAAGGACCCGCTTTTCTCACATGGCACAGGTACCATCTGCTGCAGCTGGAGAGAG ACATGCAGGAGATGCTGCAGGAGCCTTCTTTCTCCCTTCCTTACTGGAATTTTGCAACTGGGAAAAACGT CTGCGATGTCTGCACTGATGACTTGATGGGATCCAGAAGCAACTTCGATTCTACTCTTATAAGCCCCAAC TCTGTCTTTTCTCAATGGAGAGTGGTCTGTGAATCCTTGGAAGAGTACGATACCCTGGGAACACTTTGTA ACAGCACTGAGGGTGGACCAATCAGGAGAAACCCAGCTGGAAATGTAGGGAGACCAGCAGTGCAGCGTCT TCCTGAGCCACAGGATGTCACTCAGTGCTTGGAGGTCCGTGTATTTGACACACCTCCTTTTTATTCCAAT TCTACAGACAGTTTTCGAAATACAGTGGAAGGTTACAGTGCTCCCACGGGAAAATATGACCCTGCTGTTC GAAGCCTTCACAACCTGGCCCACCTCTTCCTGAATGGAACGGGAGGACAAACCCATTTGTCTCCCAATGA TCCTATTTTTGTCCTCCTGCACACTTTCACTGATGCGGTCTTTGACGAATGGCTAAGGAGGTATAACGCC GATATTTCTACCTTCCCGTTGGAAAACGCACCTATTGGACATAACAGGCAATACAACATGGTGCCATTCT GGCCTCCAGTTACCAACACAGAAATGTTTGTTACTGCTCCAGACAATCTGGGATATGCTTATGAAGTTCA ATGGCCAGGTCAGGAGTTTACTGTATCTGAAATCATTACCATTGCTGTAGTGGCTGCGTTGTTACTTGTA GCTGCCATTTTCGGGGTTGCTTCTTGTCTGATCCGTTCTAGAAGCACCAAGAATGAAGCCAACCAGCCTC TCCTCACTGATCACTATCAACGCTATGCTGAGGACTATGAGGAGCTCCCGAATCCTAACCACTCCATGGT CTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001282014
Insert Size	1614 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282014.1, NP_001268943.1
RefSeq Size	2740 bp
RefSeq ORF	1614 bp
Locus ID	22178
UniProt ID	P07147
Gene Summary	Plays a role in melanin biosynthesis (PubMed:2245916). Catalyzes the oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid (PubMed:7813420). May regulate or influence the type of melanin synthesized (PubMed:7813420, PubMed:2245916). Also to a lower extent, capable of hydroxylating tyrosine and producing melanin (PubMed:1537333).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1, 2, and 3 encode the same protein.

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