Mgrn1 (NM_001252437) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2610042J20Rik; md; mKIAA0544; nc
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228205 representing NM_001252437 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTCCATCCTGAGTCGCCGCATCGCGGGCGTAGAGGACATCGACATACAGGCCAACTCGGCCTATC GCTACCCTCCGAAGTCCGCAGGAAACTACTTTGCTTCGCATTTTTTCATGGGAGGAGAGAAATTTGACAC TCCCCATCCTGAAGGTTACCTCTTTGGAGAGAACATGGACCTGAACTTCCTGGGCAGTCGTCCAGTCCAG TTTCCCTATGTCACCCCTGCCCCCCATGAGCCAGTGAAGACACTGAGGAGCCTGGTGAACATTCGCAAAG ACTCTCTCCGGCTTGTGAGGTACAAAGAAGACGCAGACAGCCCCACGGAGGACGGTGAAAAGCCCCGCGT GCTCTACAGTCTGGAATTCACCTTTGATGCCGATGCCCGTGTGGCTATCACCATCTATTGTCAGGCGGTA GAGGAGCTGGTGAATGGCGTGGCAGTGTACAGCTGTAAGAACCCTTCTCTGCAATCTGAGACCGTCCACT ACAAGAGAGGCGTTAGCCAGCAGTTCTCCCTGCCTTCTTTCAAGATTGACTTCTCCGAGTGGAAGGATGA TGAGCTGAACTTTGACCTGGACCGGGGTGTGTTTCCAGTAGTCATCCAGGCTGTGGTGGACGAAGGGGAT GTTGTGGAAGTGACTGGCCACGCTCATGTGCTCCTGGCTGCCTTTGAGAAGCATGTGGATGGCAGCTTCT CCGTGAAGCCATTAAAGCAGAAGCAAATTGTGGACCGGGTCAGCTACCTGCTGCAGGAGATCTACGGCAT CGAGAACAAGAACAACCAGGAGACTAAGCCTTCTGATGATGAGAACAGTGACAACAGCAGTGAGTGTGTG GTGTGCCTGTCAGACCTGCGGGACACACTGATCCTGCCCTGCCGACACCTGTGCCTCTGCACTTCCTGTG CTGACACACTGCGGTACCAGGCCAACAACTGCCCCATCTGCCGGCTGCCGTTCCGAGCCCTTCTGCAGAT CCGGGCTGTGCGGAAGAAGCCAGGGGCCCTGTCTCCCATCTCCTTCAGCCCTGTCTTGGCCCAAAGTGTG GACCACGATGAGCACTCTAGTTCTGACAGTATCCCACCTGGCTATGAGCCCATCTCCTTGCTTGAGGCAC TCAATGGTCTACGAGCTGTCTCCCCGGCTATCCCATCAGCTCCCCTCTATGAGGAAATCACCTACTCAGG CATCTCGGACGGTCTTTCCCAGGCCAGTTGTCCCCTTGCTGGACTTGATCGAATCATGGAAAGTGGCCTA CAGAAGGGCAAGACACAGAGCAAGTCTCCAGACAGCACCCTGCGGTCTCCATCATTCCCCATCCATGAGG AGGACGAGGAGAAGCTCTCAGAGGACTCGGATGCTCCTCTCCCACCGAGCGGTGTGGAGCTGGTACTGCG GGAGAGCAGCTCCCCTGAGAGTTTCGGAACAGAGGAGGGAGATGAGCCGTCCCTAAAGCAAGGGAGCCGA GTACCCTCTATTGATGATGTCCTGCAAGATGGCAGTCCGCAGCATCATGGTTGCAGCCAGCCAGTCCCTC CTGCTGACATCTACCTACCAGCCCTGGGTCCTGAGTCGTGCTCTGTTGGTATAGAGGAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252437
Insert Size	1602 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252437.1, NP_001239366.1
RefSeq Size	3293 bp
RefSeq ORF	1602 bp
Locus ID	17237
UniProt ID	Q9D074
Gene Summary	E3 ubiquitin-protein ligase. Mediates TSG101 monoubiquitination at multiple sites. Plays a role in the regulation of endosome-to-lysosome trafficking. Impairs MC1R- and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production. Does not inhibit ADRB2-signaling. Does not promote MC1R ubiquitination (By similarity). Acts also as a negative regulator of hedgehog signaling (PubMed:29290584).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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