Cdc7 (NM_001271567) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI597260; Cdc7l1; muCdc7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228203 representing NM_001271567 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAGCCAATGGCGTTTTCTTCCCTTCGTGGCAGTGACAGGTGTCCGGCTGATGACTCTTTAAAAA AATATGAGCAGAGTGTTAAACTTTCAGGTATTAAAAGAGATATTGAGGAGCTTTGTGAAGCTGTACCACA ACTAGTCAATGTGTTCAAAATTAAGGACAAAATTGGAGAAGGCACCTTCAGCTCTGTTTATTTGGCCACA GCACAGTTGCAAGAAGGACATGAAGAGAAAATTGCGCTGAAACACTTAATTCCCACAAGTCATCCTATGA GGATTGCAGCAGAGCTTCAGTGTCTGACAGTTGCAGGGGGGCAAGACAATGTCATGGGACTTAAGTACTG CTTCAGAAAAAATGATCATGTGGTTATTGCTATGCCGTATCTGGAACATGAGTCCTTTTTGGACATTTTG AATTCTCTTTCCTTCCAAGAAGTTCGGGAATATATGTATAATCTTTTTGTAGCTTTGAAACGGATTCATC AGTTTGGTATTGTTCACCGTGATGTGAAGCCCAGCAATTTTTTATACAATAGACGTCTGAAAAAGTATGC CTTGGTGGACTTCGGTTTGGCCCAGGGAACCCGTGACACAAAAATAGAGCTGCTCAAGTTTGTCCAGTCT GAGGCTCAGCAGGAAGATTGTTCACGAAACAAGTATCATGGAGTCGTTGGACACAAGGGCCTGCTGAGTC GCCCGGCACCTAAAACTGTGGATCAGCAGTGTACCCCAAAAACTTCTGTCAAAAGATCCTACACACAAGT TCACATTAAGCAAGGAAAAGACGGAAAGCTCATCAAGCAATCAAAGACTGTGGACATAATATCACGAAAG CTAGCGACAAAAAAGACGGCCATTTCTACAAAAGCTATGAACAGCGTGATGAGGGAAACTGCCAGGTCCT GCCCTGCTGTCCTCACCTGCGACTGCTATGGATCAGATAGAGTCTGCAGCGTTTGCCTGTCGAGGCGGCA GCAGGTTGCCCCTAGGGCAGGCACACCAGGATTCAGAGCGCCAGAGGTCCTGACAAAGTGTCCTGACCAG ACCACAGCGATTGACATGTGGTCTGCAGGTGTCATATTCCTGTCCTTGCTCAGTGGGCGGTACCCATTTT ACAAGGCCAGTGATGACTTAACTGCTTTGGCTCAGATCATGACAATTCGAGGATCCAGGGAAACTATCCA GGCTGCTAAAGCTTTTGGCAAATCAGTTCTGTGTAGCAAAGAAGTCCCAGCACAAGACTTGAGAGCTCTC TGTGAGAGACTGCGGGGTCTAGACTCTACCACTCCCAGGTCAGCCAGTGGTCCTCCAGGGAATGCTTCCT ATGACCCTGCTGCTTCCAAGAACACTGACCACAAAGCATCCCGTGTACAGGCTGCTCAGGCACAGCACTC AGAGGACTCCTTGTATAAAAGGGACAACGATGGCTATTGGAGTCATCCCAAAGACTGCACTTCCAACTCA GAAGGCTGGGACTCAGTACCTGATGAAGCCTATGACCTGCTCGACAAGCTTCTGGACCTAAACCCAGCTT CAAGGATAACAGCAGAAGCAGCCTTATTACATGCGTTCTTTAAAGATATGTGCTCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271567
Insert Size	1599 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271567.1, NP_001258496.1
RefSeq Size	2872 bp
RefSeq ORF	1599 bp
Locus ID	12545
UniProt ID	Q9Z0H0
Gene Summary	Seems to phosphorylate critical substrates that regulate the G1/S phase transition and/or DNA replication. Can phosphorylates MCM2 and MCM3.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR and lacks an alternate in-frame exon in the coding region compared to variant 1. It encodes isoform 2, which is shorter than isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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