Eya2 (NM_001271962) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Eab1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228200 representing NM_001271962 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTAGAAGTGGTGACCTCACCCAGCCTCGCAACAAGCAGTGACTGGAGCGAGCACGGTGCTGCCGTGG GGACGCTGAGTGACAGGGAAGGCATCGCCAAATCAGCGGCTCTGAGTGTGCCTCAGCTCTTTGTGAAGTC TCATCCACGTGTCCCTCCTGGTCAGTCCTCCACAGCCATGGCGGCCTATGGCCAGACACAGTACAGCACA GGCATTCAGCAGGCACCACCCTATACAGCGTACCCAACTCCGGCGCAAGCCTATGGAATCCCCCCTTACA GCATCAAGACAGAAGACAGTTTGAATCACTCCCCCAGCCAGAGCGGGTTCCTGAGCTATGGACCGAGCTT CAGCACCGCGCCTGCTGGACAGAGCCCCTACACCTACCCCGTGCACAGCACCGCTGGGCTCTATCAAGGC GCCAACGGACTGACCAACACCGCTGGATTTGGGAGCGTGCACCAGGATTATCCGTCCTACCCCAGCTTTT CACAGAACCAGTACCCCCAGTATTTCAGCCCATCATACAACCCGCCCTACGTCCCTGCCAGCAGCCTCTG CTCCTCGCCCCTCTCCACGTCCACCTACGTCCTCCAGGAGGCTCCTCACAATGTCCCCAGCCAGAGTTCT GAGTCCCTGGCCGGAGACTACAACACACACAACGGACCCTCCACACCAGCAAAGGAGGGTGACACAGAGA GGCCACATCGAGCCTCGGATGGGAAGCTACGGGGCCGGTCAAAGAGAAATAGTGACCCTTCCCCAGCAGG AGACAATGAAATCGAGCGCGTGTTCGTCTGGGACCTGGACGAGACAATCATTATCTTCCACTCCCTGCTC ACAGGGACGTTTGCATCCAGATACGGGAAGGACACCACGACGTCTGTGCGCATTGGCCTGATGATGGAGG AGATGATCTTCAACCTTGCTGACACACACCTGTTCTTCAATGACCTGGAGGACTGTGACCAAATCCACGT GGATGATGTCTCATCCGATGACAATGGTCAGGATTTAAGCACATACAACTTCTCCACTGATGGCTTCCAC AGCACGGCGCCAGGAGCCAGCTTGTGCCTGGGTACAGGTGTTCATGGCGGTGTGGACTGGATGAGGAAAC TGGCCTTCCGCTACCGTCGTGTGAAGGAGATGTACAACACCTACCGCAACAACGTGGGTGGCTTGATAGG TGCTCCCAAAAGAGAGACCTGGCTGCAGCTGCGCGCCGAGCTGGAGGCCCTGACTGACCTCTGGCTCACC CACTCCCTGAAAGCCCTCAATCTCATCAACTCTCGACCCAACTGTGTCAATGTGTTGGTCACCACCACGC AACTGATCCCTGCATTGGCCAAGGTCCTGCTGTACGGTCTGGGCTCCGTGTTCCCCATCGAGAACATCTA CAGTGCGACCAAGACAGGCAAGGAGAGCTGCTTCGAAAGAATCATGCAGAGGTTTGGCCGCAAAGCTGTC TACATTGTGATAGGCGACGGGGTAGAGGAAGAGCAAGGAGCCAAAAAGCACAACATGCCTTTCTGGAGGA TATCCTGTCATGCTGACCTGGAGGCTCTAAGGCATGCCCTGGAACTGGAGTATCTATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271962
Insert Size	1599 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271962.1, NP_001258891.1
RefSeq Size	2283 bp
RefSeq ORF	1599 bp
Locus ID	14049
UniProt ID	O08575
Gene Summary	This gene encodes a member of the eyes absent protein family. The encoded protein is a tyrosine phosphatase which acts as a transcriptional activator during development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data.

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