Rbm47 (NM_001291226) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	9530077J19Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228146 representing NM_001291226 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGCTGAAGATTCCGCCACAGCCATGAACAGCGATCCAACAGTGGGGTCCTCCACCAAGGTACCGG AGGGTGTGGCGGGCGCGCCCAATGAGGCTGCACTGTTGGCACTGATAGAGCGCACCGGCTACACCATGGT ACAAGAGAACGGGCAGCGCAAGTACGGTGGACCCCCGCCCGGCTGGGAGGGCCCGCATCCCCAGCGTGGC TGTGAGGTCTTCGTGGGCAAGATCCCACGTGACGTGTATGAAGATGAGCTGGTGCCTGTGTTTGAGACCG TAGGTCGCATCTATGAGCTGCGACTCATGATGGATTTCGATGGCAAGAACCGCGGCTATGCCTTCGTGAT GTACTGCCACAAGCATGAGGCCAAGCGAGCCGTTCGGGAACTCAACAACTACGAGATCCGCCCCGGCCGT CTGCTGGGTGTGTGCTGTAGTGTGGACAACTGCCGCCTCTTCATTGGTGGCATCCCCAAGATGAAGAAGC GCGGGGAGATCCTGGAGGAGATCGCCAAGGTCACCGAGGGCGTACTCAACGTGATTGTGTATGCCAGTGC GGCAGACAAGATGAAAAACCGAGGCTTCGCCTTTGTGGAGTATGAGAGCCATCGTGCTGCAGCCATGGCA CGCCGCAAGCTCATGCCGGGTCGGATCCAACTGTGGGGCCACCAGATTGCTGTGGACTGGGCCGAGCCAG AGATTGACGTGGACGAAGATGTGATGCAGACAGTGAAGATCCTCTACGTGCGCAACCTCATGATCGAGAC CACCGAGGAGACCATCAAGAAGAGCTTTGGCCAGTTCAACCCAGGCTGCGTGGAAAGGGTCAAGAAGATC CGTGACTACGCCTTCGTGCACTTCACTAGCCGTGAGGATGCTGTGCATGCTATGAACAACCTCAACGGCA CAGAGCTGGAGGGATCCTGCTTAGAAGTCACGCTGGCCAAGCCCGTGGACAAGGAACAGTACTCCCGCTA CCAGAAGGCAGCCAAGGGTGGTGGTGGCTCGGCTGAGGCCGTAGCGCAGCAACCCAGCTACGTGTACTCC TGTGACCCCTACACGTTGGCCTACTACGGCTACCCCTACAACGCTCTCATCGGGCCCAACCGGGACTACT TCGTGAAAATGGCCATCCCTGCCATCGGTGCTCAGTACTCCATGTTCCAGGCAGCCCCAGCCCCGAAAAT AATCGAAGATGGCAAGATCCACACAATGGAACACATGATCAGCCCCATTGCGGTGCAGCCTGACCCCGCC ACTGCTGCTGCCGCTGCAGCCGCTGCAGCCGCTGCTGCCGTCATTCCTGCGGTATCCACACCGCCACCTT TCCAGGGCCGTCCAATAACTCCTGTGTACACCGTGGCACCAAATGTTCAGAGAATCCCCACCGCCGGCAT CTACGGGGCCAGCTACGTCCCCTTTGCTGCTCCCGCCACTGCCACAATCGCCACACTACAGAAGAACGCC GCGGCTGCCGTGTATGGGGGCTACGCCGGCTACATACCTCAGGCCTTCCCCGCTGCTCTCCAGGTGCCCA TCCACGACGTCTACCAGACTTACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001291226
Insert Size	1566 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291226.1, NP_001278155.1
RefSeq Size	4940 bp
RefSeq ORF	1566 bp
Locus ID	245945
UniProt ID	Q91WT8

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