Ppil2 (NM_001252445) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	0610009L05Rik; 1700016N17Rik; 4921520K19Rik; 4930511F14Rik; AA589416; C130078A06Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228144 representing NM_001252445 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAAGCGACAGCACCAGAAGGACAAGATGTATATCACTTGTGCTGAGTACACTCATTTCTATGGTG GCAGGAAGCCAGATATCTCACAGACAAGTTTTCGCCGCTTACCTTTTGACCACTGCAGTCTCTCTCTCCA GCCTTTTGTCTACCCAGTCTGCACCCCAGAAGGTGTCGTCTTTGACTTGCTGAACATTGTTCCCTGGCTT AAGAAGTATGGGACGAATCCCAGCACTGGAGAGAAACTTGATGGGAAGTCCTTGATCAAGCTGAACTTCG CAAAGAACAGCGAAGGGCAGTACCACTGTCCAGTGCTGTATTCCGTGTTCACTGACAACACCCATATTGT GGCCATCAGGACAACTGGCAATGTCTACACCTATGAGGCAGTGGAGCAGCTAAACATCAAGGCCAAGAAC TTGCGGGATCTGTTGACTGATGAGCCCTTTTCCAGGCAAGACATCATCACCCTGCAGGACCCCACCAATT TGGACAAATTCAATGTTAGCAACTTCTTCCATGTGAAGAATAACATGAGAATAATAGACCCAGATGAGGA AAAGGCCAAACAAGACCCATCTTATTATTTGAAAAACACAAATTCGGAGACGAGAGAGACGCTACAGGAG CTCTACAAAGAGTTCAAAGGAGATGAGATTTTAGCAGCTACCATGAGGCCACCTGAGAAGAAGAAGGTGG ACCAACTGAATGCGGCCCACTACTCCACAGGGAAGGTCAGTGCATCCTTCACCTCTACTGCCATGGTGCC CGAGACCACGCATGAAGCAGCTGTCATTGATGAAGATGTACTGCGCTACCAGTTTGTGAAGAAAAAGGGC TATGTAAGGCTTCACACCAACAAGGGCGACCTTAACTTAGAGCTGCACTGTGACCTGACACCAAAAACCT GTGAAAACTTCATCAAGCTCTGCAAGAAACAGTATTATGATGGTACCATCTTTCACAGGTCCATCAGGAA CTTTGTGATCCAGGGCGGTGACCCCACAGGTACAGGCACAGGTGGAGAGTCATTCTGGGGCAAGCCTTTC AAAGATGAGTTCCGTCCCAACCTTTCACACACGGGCCGTGGGGTGCTCAGCATGGCCAATTCGGGGCCCA ACACCAACAAATCTCAGTTCTTCATCACATTCCGATCCTGCGCTTACCTGGATAAGAAGCATACCATCTT TGGACGGGTTGTTGGGGGCTTTGACACGCTGACAGCCATGGAGAATGTGGAGAGTGACCCTAAAACTGAC CGTCCTAAGGAGGAAGTGCTCATATGTACAACCACAGTGTTTGTGGACCCCTATGAGGAGGCTGATGCCC AGATTGCCCAGGAACGGAAGAAGACACAGCATCAGGTGGATCCAGAGGCCAAGGTCAAGATGAGTCAGCC CCAGCCTGGAAACCAGGGACCCCAGACATACCGCCAGGGGGTGGGCAAGTACATCCACCCTGCGGCCACG AAACGATCAGCAGAGGAAGAACCATCGACCAGCACTGCCACCCCCACGGCCAAGAAAAGGCCCAGTCGGG GCTTTGGGGACTTCAGCTCCTGGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252445
Insert Size	1566 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252445.1, NP_001239374.1
RefSeq Size	1812 bp
RefSeq ORF	1566 bp
Locus ID	66053
UniProt ID	Q9D787
Gene Summary	Has a ubiquitin-protein ligase activity acting as an E3 ubiquitin protein ligase or as an ubiquitin-ubiquitin ligase promoting elongation of ubiquitin chains on substrates. By mediating 'Lys-48'-linked polyubiquitination of proteins could target them for proteasomal degradation. May also function as a chaperone, playing a role in transport to the cell membrane of BSG/Basigin for instance. Probable inactive PPIase with no peptidyl-prolyl cis-trans isomerase activity.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 3' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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