Lrrtm4 (NM_001282102) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	7530419J18Rik; A230052N11
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228138 representing NM_001282102 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGGTTTCCGTCTAATCACGCAGCTGAAAGGCATGAGTGTGTTTCTGGTGCTATTTCCCACCCTGC TGCTGGTTATGCTGACGGGGGCTCAGAGAGCTTGCCCAAAGAACTGCAGATGCGATGGCAAGATTGTATA CTGTGAGTCTCACGCCTTCGCAGACATCCCTGAGAACATTTCTGGAGGGTCACAAGGTTTATCCTTAAGG TTCAACAGCATTCAGAAACTCAAATCCAATCAGTTTGCCGGCCTTAACCAGCTTATATGGCTTTATCTTG ACCATAATTACATTAGCTCAGTGGACGAAGATGCCTTTCAAGGGATCCGCAGACTGAAAGAATTAATTCT AAGCTCCAACAAAATTACCTATCTGCACAATAAAACATTTCACCCAGTCCCCAACCTCCGCAACCTGGAT CTCTCCTACAACAAACTTCAGACCTTACAGTCTGAGCAGTTTAAAGGCCTCCGGAAACTCATCATTTTGC ACTTGCGATCTAATTCTCTCAAGACCGTGCCCATACGGGTTTTTCAAGACTGCCGGAACCTTGACTTTCT GGATTTGGGTTATAATCGGCTCCGAAGCTTATCCCGAAACGCGTTTGCCGGTCTTCTGAAGTTAAAAGAG CTCCACCTGGAGCATAATCAGTTTTCAAAGATCAACTTTGCTCATTTTCCTCGTCTCTTCAACCTGCGAT CCATCTACCTGCAATGGAACAGGATCCGCTCTGTCAGTCAAGGCCTAACATGGACCTGGAGTTCCTTGCA CACCTTGGATTTATCAGGGAATGACATCCAAGCAATCGAGCCTGGCACATTTAAATGCCTCCCCAATTTG CAAAAATTGAATTTGGATTCCAACAAGCTCACCAATGTCTCCCAGGAGACTGTCAATGCATGGATATCCT TAATATCCATCACCTTGTCTGGAAACATGTGGGAATGTAGTCGGAGCATCTGTCCTCTATTCTACTGGCT GAAGAATTTCAAAGGGAATAAAGAAAGTACCATGATATGTGCAGGGCCTAAGCACATACAGGGTGAGAAG GTTAGCGATGCCGTGGAGACTTACAACATCTGCTCTGATGTCCAGGTGGTCAACACAGAACGATCACATC TGGCGCCTCAAACTCCCCAGAAGCCCCCGTTCATCCCCAAACCTACCATCTTCAAACCGGATGCAGTCCC AGCCACCCTAGAGGCAGTGAGCCCATCCCCGGGCTTTCAAATTCCAGGTACGGATCATGAGTACGAGCAC GTGTCGTTTCACAAAATCATCGCAGGGAGCGTGGCTCTGTTCCTCTCCGTGGCCATGATTCTCCTGGTGA TCTATGTGTCTTGGAAACGGTACCCAGCCAGCATGAAACAACTCCAACAGCACTCACTTATGAAGAGGAG ACGGAAAAAGGCGAGGGAGTCGGAGAGACAGATGAATTCCCCTTTACAGGAATACTACGTGGACTACAAA CCGACGAACTCTGAGACCATGGATATATCGGTTAATGGATCTGGTCCCTGCACATATACCATCTCTGGCT CCAGGGAGTGTGAGGTATGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001282102
Insert Size	1560 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282102.1, NP_001269031.1
RefSeq Size	3629 bp
RefSeq ORF	1560 bp
Locus ID	243499
Gene Summary	May play a role in the development and maintenance of the vertebrate nervous system. Exhibits strong synaptogenic activity, restricted to excitatory presynaptic differentiation (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate 5' terminal exon, and thus differs in the 5' UTR and 5' coding region, compared to variant 1. These differences result in translation initiation at an alternate AUG and result in an isoform (2) with a longer and distinct N-terminus, compared to isoform 1.

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