Tnk2 (NM_001289443) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Ack; Ack-1; Ack1; Cdgip; Pyk1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228134 representing NM_001289443 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCCTCCTGACCTGCTGAGTGTGGAACTGAGCACCTCCCGACCCACCCAGCACCTAGGACGGGTGA AAAAGCCAACATACGACCCTGTGAGTGAGGACCCAGACCCCCTGTCCAGCGACTTCAAGAGGCTTGGCCT GAGGAAGCCAGCCCTGCCTCGAGGGCTGTGGCTGGCAAAGCCCTCAGCCCGAGTGCCAGGCACCAAGGCA GACCGCAGCAGTGGGGGTGAGGTCACACTCATCGACTTCGGTGAGGAGCCTGTGGTCCCAACCCCTCGGC CCTGTGCACCCTCCTTGGCACAGTTGGCCATGGATGCCTGCTCCTTGCTGGACAAGACACCACCACAGAG CCCCACACGGGCACTGCCACGACCTTTACACCCCACCCCTGTGGTAGACTGGGACGCGCGTCCGCTGCCC CCGCCCCCTGCCTATGACGACGTGGCCCAGGATGAGGATGACTTTGAGGTCTGCTCCATCAACAGCACGC TAGTAGGTGCAGGCCTCCCTGCTGGGCCTAGCCAAGGCGAGACCAATTACGCCTTTGTACCTGAGCAGGC GCAGATGCCCCCTGCCCTGGAGGATAACCTGTTCCTTCCACCCCAGGGCGGAGGCAAGCCGCCCAGCTCA GTGCAGACTGCAGAGATTTTCCAGGCACTGCAGCAGGAGTGTATGCGGCAGCTACAGGTCCCCACTGGCC AGCTGACCCCCTCCCCGACCCCAGGAGGTGATGACAAGCCCCAGGTGCCACCCCGGGTACCTATTCCCCC TCGGCCCACGCGTCCACGTGTGGAGCTATCTCCAGCTCCCTCGGGTGAGGAAGAGACAAGCCGGTGGCCT GGACCTGCCTCGCCCCCCCGAGTGCCTCCCCGGGAACCTCTGTCTCCTCAAGGTTCAAGGACCCCAAGCC CCCTAGTGCCACCTGGCAGCTCTCCACTACCGCATCGGCTCTCTAGCTCACCTGGAAAGACCATGCCCAC CACTCAAAGCTTTGCCTCAGACCCTAAGTATGCCACTCCACAAGTGATCCAGGCTCCTGGCCCACGGGCA GGCCCCTGCATCCTGCCCATTGTCCGCGATGGCAGGAAGGTCAGCAGCACTCATTACTACCTGCTGCCTG AGCGCCCTCCTTACCTGGAACGCTATCAGCGCTTCCTGCGGGAGGCCCAGAGCCCGGAAGAGCCGGCCGC CCTGCCTGTGCCCCCGCTGTTGCCCCCGCCCAGTACTCCAGCCCCTGCTGCCCCCACTGCCACCGTCAGA CCTATGCCTCAGGCCGCCCCAGACCCAAAGGCCAACTTCTCCACCAATAACAGCAACCCAGGGGCACGGC CACCATCCCTGAGGGCCACGGCTCGGCTGCCACAGAGGGGCTGCCCAGGGGACGGGCAAGAGGCTGCTCG GCCAGCAGACAAGGTCCAGATGGTGGAGCAGCTCTTTGGGCTGGGTCTTCGGCCACGGGTGGAGTGCCAC AAGGTCCTAGAGATGTTCGACTGGAACCTAGAGCAAGCCGGCTGTCACCTTCTGGGCTCCTGTGGCCCTG CTCATCACAAACGCTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001289443
Insert Size	1557 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289443.1, NP_001276372.1
RefSeq Size	2721 bp
RefSeq ORF	1557 bp
Locus ID	51789
UniProt ID	O54967
Gene Summary	Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development. Activates AKT1 by phosphorylating it on 'Tyr-176'. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Phosphorylates MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to the control of AXL receptor levels. Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in its 5' UTR, lacks part of the 5' coding region, lacks two in-frame exons in the central coding region, and uses a downstream in-frame start codon, compared to variant 1. The encoded isoform (3) is shorter, compared to isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.