Acvrl1 (NM_001277255) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Acvrlk1; AI115505; AI427544; Alk1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228132 representing NM_001277255 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGGAAATCTCACCACATCTCTTCTCCTATCTCCAAGGACCATGACCTTGGGGAGCTTCAGAAGGG GCCTTTTGATGCTGTCGGTGGCCTTTGGCCTAACCAGGGGAGACTTGGCGAAGCCTTCCAAGCTGGTGAA CTGCACTTGTGAGAGCCCACACTGCAAGAGACCATTCTGCCAGGGGTCATGGTGCACAGTGGTGCTGGTT CGGGAGCAGGGCAGGCACCCCCAGGTCTATCGGGGCTGCGGGAGCCTGAACCAGGAGCTCTGCTTGGGAC GTCCCACAGAGTTTCTGAACCATCACTGCTGCTATAGATCCTTCTGCAACCACAACGTGTCTCTGATGCT GGAGGCCACCCAAACTCCTTCGGAGGAGCCAGAAGTTGATGCCCATCTGCCTCTGATCCTGGGTCCTGTG CTGGCCTTGCCGGTCCTGGTGGCCCTGGGTGCTCTAGGCTTGTGGCGTGTCCGGCGGAGGCAGGAGAAGC AGCGGGATTTGCACAGTGACCTGGGCGAGTCCAGTCTCATCCTGAAGGCATCTGAACAGGCAGACAGCAT GTTGGGGGACTTCCTGGACAGCGACTGTACTACGGGCAGCGGCTCGGGGCTCCCCTTCTTGGTGCAGAGG ACGGTAGCTCGGCAGGTTGCGCTGGTAGAGTGTGTGGGAAAGGGCCGATATGGTGAGGTGTGGCGCGGTT CGTGGCATGGCGAGAGTGTGGCGGTCAAGATTTTCTCCTCACGAGATGAGCAGTCCTGGTTCCGGGAGAC GGAGATCTACAACACAGTTCTGCTTAGACACGACAACATCCTAGGCTTCATCGCCTCCGACATGACTTCG CGGAACTCGAGCACGCAGCTGTGGCTCATCACCCACTACCATGAACACGGCTCCCTCTATGACTTTCTGC AGAGGCAGACGCTGGAGCCCCAGTTGGCCCTGAGGCTAGCTGTGTCCGCGGCCTGCGGCCTGGCGCACCT ACATGTGGAGATCTTTGGCACTCAAGGCAAACCAGCCATTGCCCATCGTGACCTCAAGAGTCGCAATGTG CTGGTCAAGAGTAACTTGCAGTGTTGCATTGCAGACCTGGGACTGGCTGTGATGCACTCACAAAGCAGCG ATTACCTGGATATCGGCAACAACCCCCGAGTGGGTACCAAAAGATACATGGCACCCGAGGTGCTGGATGA GCACATCCGCACAGACTGCTTTGAGTCGTACAAGTGGACAGACATCTGGGCCTTTGGCCTAGTGCTATGG GAGATCGCCCGGCGGACCATCATCAATGGCATTGTGGAGGATTACAGGCCACCTTTCTATGACATGGTAC CCAATGACCCCAGTTTTGAGGACATGAAAAAGGTGGTGTGCGTTGACCAGCAGACACCCACCATCCCTAA CCGGCTGGCTGCAGATCCGGTCCTCTCCGGGCTGGCCCAGATGATGAGAGAGTGCTGGTACCCCAACCCC TCTGCTCGCCTCACCGCACTGCGCATAAAGAAGACATTGCAGAAGCTCAGTCACAATCCAGAGAAGCCCA AAGTGATTCACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001277255
Insert Size	1554 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001277255.1, NP_001264184.1
RefSeq Size	3663 bp
RefSeq ORF	1554 bp
Locus ID	11482
Gene Summary	Type I receptor for TGF-beta family ligands BMP9/GDF2 and BMP10 and important regulator of normal blood vessel development. On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. May bind activin as well.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) differs in the 5' UTR and coding sequence and uses an upstream in-frame AUG translation start codon compared to variant 1. The resulting isoform (b) is longer at the N-terminus compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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