Gba (NM_001077411) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	betaGC; GBA1; GC; GCase; GLUC
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228122 representing NM_001077411 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCCAGGCTCATCGGATTCTTCCTATTTCAGGCGGTATCTTGGGCATATGGTGCCCAACCCTGCA TCCCCAAAAGCTTTGGCTACAGCTCAGTGGTCTGTGTCTGTAATGCATCGTACTGTGACTCTCTTGACCC CGTGACCTTACCGGCTCTGGGTACCTTCAGCCGTTACGAGAGCACTCGACGTGGACGTCGGATGGAGCTG AGTGTCGGGGCCATCCAGGCCAATCGCACTGGCACAGGGTTACTACTCACTTTGCAGCCAGAAAAGAAGT TCCAGAAAGTGAAAGGATTTGGAGGCGCCATGACAGACGCCACTGCGCTCAACATCCTTGCTTTGTCCCC ACCTACTCAGAAGCTGCTACTCAGATCCTACTTCTCTACCAACGGAATTGAATATAACATCATCCGGGTA CCCATGGCCAGTTGTGACTTCTCCATCCGTGTCTATACCTATGCTGACACCCCTAACGACTTCCAGTTAT CCAACTTCAGCCTCCCAGAAGAAGACACCAAGCTCAAGATACCCCTGATTCACCAAGCCCTGAAGATGTC CTCACGCCCCATTTCACTCTTTGCCAGTCCCTGGACATCACCCACTTGGCTCAAGACCAATGGAAGAGTG AATGGGAAGGGGTCGCTCAAGGGTCAGCCAGGGGATATCTTTCACCAGACCTGGGCCAATTACTTTGTCA AGTTTCTGGATGCTTATGCTAAGTATGGCCTAAGATTCTGGGCAGTGACAGCGGAGAATGAACCTACAGC AGGGCTCTTCACGGGGTACCCCTTCCAGTGCCTGGGCTTCACTCCTGAACACCAGAGAGACTTCATTTCC CGTGACCTAGGGCCAGCCCTTGCCAACAGTTCCCATGATGTGAAGCTACTCATGCTAGATGACCAACGCT TGCTGCTACCCCGCTGGGCAGAGGTGGTGCTCTCTGATCCAGAGGCAGCTAAGTACGTTCATGGCATTGC TGTTCACTGGTATATGGATTTCCTGGCTCCAGCCAAAGCTACTTTAGGAGAGACACACCGCTTGTTCCCC AACACAATGCTCTTTGCTTCGGAGGCCTGCGTGGGCTCCAAGTTCTGGGAACAGAGTGTTCGGTTGGGCT CCTGGGATCGAGGGATGCAGTACAGTCACAGCATCATTACGAACCTCCTTTACCACGTAACTGGATGGAC GGACTGGAACCTTGCCCTGAATCCTGAAGGAGGGCCCAACTGGGTCCGCAACTTTGTCGATAGCCCCATT ATTGTCGACATCCCCAAAGACGCATTTTACAAACAGCCCATGTTCTACCATCTTGGCCACTTCAGCAAGT TCATTCCCGAGGGATCCCAGAGAGTGGCGTTGGTGGCCAGTGAGAGCACTGACTTGGAAACAGTAGCACT GTTACGCCCTGACGGCTCTGCAGTTGTGGTCGTGTTAAACCGATCTTCGGAGGATGTCCCTCTTACCATC AGTGATCCTGACCTGGGCTTCCTGGAGACCGTGTCACCTGGCTACTCCATTCACACTTACCTGTGGCGTC GCCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001077411
Insert Size	1548 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001077411.2, NP_001070879.1
RefSeq Size	2069 bp
RefSeq ORF	1548 bp
Locus ID	14466
UniProt ID	P17439
Gene Summary	Glucosylceramidase that catalyzes, within the lysosomal compartment, the hydrolysis of glucosylceramide/GlcCer into free ceramide and glucose (PubMed:24211208). Thereby, plays a central role in the degradation of complex lipids and the turnover of cellular membranes (PubMed:27378698). Through the production of ceramides, participates to the PKC-activated salvage pathway of ceramide formation (By similarity). Also plays a role in cholesterol metabolism (PubMed:24211208). May either catalyze the glucosylation of cholesterol, through a transglucosylation reaction that transfers glucose from glucosylceramide to cholesterol (PubMed:24211208). The short chain saturated C8:0-GlcCer and the mono-unsaturated C18:0-GlcCer being the most effective glucose donors for that transglucosylation reaction (By similarity). Under specific conditions, may alternatively catalyze the reverse reaction, transferring glucose from cholesteryl-beta-D-glucoside to ceramide (By similarity). Finally, may also hydrolyze cholesteryl-beta-D-glucoside to produce D-glucose and cholesterol (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) represents the shortest transcript and encodes the functional protein. Variants 1 and 2 encode the same protein.

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