Gba (NM_001077411) Mouse Untagged Clone

CAT#: MC228122

Gba (untagged) - Mouse glucosidase, beta, acid (Gba), transcript variant 2



  "NM_001077411" in other vectors (1)

CNY 4,940.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms betaGC; GBA1; GC; GCase; GLUC
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC228122 representing NM_001077411
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCCAGGCTCATCGGATTCTTCCTATTTCAGGCGGTATCTTGGGCATATGGTGCCCAACCCTGCA
TCCCCAAAAGCTTTGGCTACAGCTCAGTGGTCTGTGTCTGTAATGCATCGTACTGTGACTCTCTTGACCC
CGTGACCTTACCGGCTCTGGGTACCTTCAGCCGTTACGAGAGCACTCGACGTGGACGTCGGATGGAGCTG
AGTGTCGGGGCCATCCAGGCCAATCGCACTGGCACAGGGTTACTACTCACTTTGCAGCCAGAAAAGAAGT
TCCAGAAAGTGAAAGGATTTGGAGGCGCCATGACAGACGCCACTGCGCTCAACATCCTTGCTTTGTCCCC
ACCTACTCAGAAGCTGCTACTCAGATCCTACTTCTCTACCAACGGAATTGAATATAACATCATCCGGGTA
CCCATGGCCAGTTGTGACTTCTCCATCCGTGTCTATACCTATGCTGACACCCCTAACGACTTCCAGTTAT
CCAACTTCAGCCTCCCAGAAGAAGACACCAAGCTCAAGATACCCCTGATTCACCAAGCCCTGAAGATGTC
CTCACGCCCCATTTCACTCTTTGCCAGTCCCTGGACATCACCCACTTGGCTCAAGACCAATGGAAGAGTG
AATGGGAAGGGGTCGCTCAAGGGTCAGCCAGGGGATATCTTTCACCAGACCTGGGCCAATTACTTTGTCA
AGTTTCTGGATGCTTATGCTAAGTATGGCCTAAGATTCTGGGCAGTGACAGCGGAGAATGAACCTACAGC
AGGGCTCTTCACGGGGTACCCCTTCCAGTGCCTGGGCTTCACTCCTGAACACCAGAGAGACTTCATTTCC
CGTGACCTAGGGCCAGCCCTTGCCAACAGTTCCCATGATGTGAAGCTACTCATGCTAGATGACCAACGCT
TGCTGCTACCCCGCTGGGCAGAGGTGGTGCTCTCTGATCCAGAGGCAGCTAAGTACGTTCATGGCATTGC
TGTTCACTGGTATATGGATTTCCTGGCTCCAGCCAAAGCTACTTTAGGAGAGACACACCGCTTGTTCCCC
AACACAATGCTCTTTGCTTCGGAGGCCTGCGTGGGCTCCAAGTTCTGGGAACAGAGTGTTCGGTTGGGCT
CCTGGGATCGAGGGATGCAGTACAGTCACAGCATCATTACGAACCTCCTTTACCACGTAACTGGATGGAC
GGACTGGAACCTTGCCCTGAATCCTGAAGGAGGGCCCAACTGGGTCCGCAACTTTGTCGATAGCCCCATT
ATTGTCGACATCCCCAAAGACGCATTTTACAAACAGCCCATGTTCTACCATCTTGGCCACTTCAGCAAGT
TCATTCCCGAGGGATCCCAGAGAGTGGCGTTGGTGGCCAGTGAGAGCACTGACTTGGAAACAGTAGCACT
GTTACGCCCTGACGGCTCTGCAGTTGTGGTCGTGTTAAACCGATCTTCGGAGGATGTCCCTCTTACCATC
AGTGATCCTGACCTGGGCTTCCTGGAGACCGTGTCACCTGGCTACTCCATTCACACTTACCTGTGGCGTC
GCCAGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001077411
Insert Size 1548 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001077411.2, NP_001070879.1
RefSeq Size 2069 bp
RefSeq ORF 1548 bp
Locus ID 14466
UniProt ID P17439
Gene Summary Glucosylceramidase that catalyzes, within the lysosomal compartment, the hydrolysis of glucosylceramide/GlcCer into free ceramide and glucose (PubMed:24211208). Thereby, plays a central role in the degradation of complex lipids and the turnover of cellular membranes (PubMed:27378698). Through the production of ceramides, participates to the PKC-activated salvage pathway of ceramide formation (By similarity). Also plays a role in cholesterol metabolism (PubMed:24211208). May either catalyze the glucosylation of cholesterol, through a transglucosylation reaction that transfers glucose from glucosylceramide to cholesterol (PubMed:24211208). The short chain saturated C8:0-GlcCer and the mono-unsaturated C18:0-GlcCer being the most effective glucose donors for that transglucosylation reaction (By similarity). Under specific conditions, may alternatively catalyze the reverse reaction, transferring glucose from cholesteryl-beta-D-glucoside to ceramide (By similarity). Finally, may also hydrolyze cholesteryl-beta-D-glucoside to produce D-glucose and cholesterol (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) represents the shortest transcript and encodes the functional protein. Variants 1 and 2 encode the same protein.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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