Rnpc3 (NM_001287015) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2810441O16Rik; AI447568; C030014B17Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228115 representing NM_001287015 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGGCCTGAGCCGCCGATGCCGCTGTCCCGAGGCGGCCCAGGCTCCGCTTCGCTTTCTCCGCCCC GGGGAGACCGCACCCTCCTCGTCAGGCACCTGCCGGCCGAGCTGACGGCTGAGGAGAAGGAGGACTTACT GAAGTACTTCGGGGCGCAGTCGGTGCGCGTCCTGTCAGATAAGGGGCGCCTGAAACACACAGCATTTGCT ACTTTCCCTAATGAAAAAGCAGCAATAAAGGCACTGACGAGACTCCATCAGCTGAAACTTCTAGGTCATA CTTTAGTTGTTGAATTTGCAAAAGAGCAAGATCGAGTTCACTCCCCATGTTCCACCTCAAATACAGAGAA AAAGAAAAGGTTGGATGACACTGTGGAAAATGATAAAGAGAAGAAAGAACCTGACATTTTAACAGTAGAA AATGGAATTGCACCAAATCATGGGCTGACTTTTCCTCTGAATTCATGCCTTAAATATATGTATCCTCCAC CATCAAGCACAATCCTCGCAAATATAGTAAATGCTCTGGCAAGTGTCCCAAAATTCTATGTACAGGTGCT TCATCTTATGAATAAAATGAATTTGCCCACACCATTTGGACCGATTACTGCACGACCTCCTATGTATGAG GACTATATGCCATTGCATGCACCCCTTCCACCTACATCCCCTCAACCGCCTGAGGAGCCTCCCTTACCAG ATGAGGATGAGGATTTATCTAGCAAAGAATCAGAATATGAAAGCAGTGATGAGGAGGACCGACAGAGAAT GAACAGATTAATGGAACTAGCAAATCTTCAGCCCAAAAGACCCAAAACAGAAAAGCCACGCCACGTGAGA AAAAAGCGAAAAATAAAGGATATGTTGAATATACCTTCATCAGCTTCACATAGTTTGCATCCTGTGCTGT TACCTTCGGATGTATTTGACCAGCCACAATCTGTAGGTAATAAAAAAATTGAGTTCAATATATCAACCAA CATGCCTGCAGCATTTAACAAAGATTTGGAGACACAACCAAATAATGAAGAAGAAAATTCTGATTCACCA GACACTGGACTTGATTCAAATACAGGATTTGGCAAAATCTTCCCTAAACCTAATTTGAACATCACAGAAG AGATTACAGAAGACTCTGATGAAATACCTTCACAATTTATTTCTAGAAAAGAGTTGGAAAAGGGTCGTAT TTCTAGAGAAGAAATGGAAACACTTTCAGTTTTTAGAAGTTATGAACCTGGTGAACCAAACTGCAGAATT TATGTAAAGAATTTAGCTAGACATGTTCAGGAAAAGGACCTTAAATTTATATTTGGAAGATACGTTGACT TTTCTTCAGAAACACAGCGGATCATGTTTGATATTCGCTTAATGAAAGAAGGACGCATGAAAGGACAAGC TTTTGTTGGACTTCCAAATGAAAAAGCAGCCGCTAAAGCGTTGAAAGAAGCTAATGGCTATGTTCTTTTT GGAAAACCCATGGTGGTTTTTGCTCGATCTGCTAGACCAAAACAGGATTCTAAAGAAGGAAAACGGAAGT GA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001287015
Insert Size	1542 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001287015.1, NP_001273944.1
RefSeq Size	3885 bp
RefSeq ORF	1542 bp
Locus ID	67225
UniProt ID	Q3UZ01
Gene Summary	Participates in pre-mRNA U12-dependent splicing, performed by the minor spliceosome which removes U12-type introns. U12-type introns comprises less than 1% of all non-coding sequences. Binds to the 3'-stem-loop of m(7)G-capped U12 snRNA (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. It encodes isoform b which is shorter than isoform a.

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