Sars (NM_001204979) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Sars1; serRS; Strs
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228113 representing NM_001204979 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCTGGACCTGGATTTGTTTCGGGTGGATAAAGGAGGGGACCCAGCCCTCATTCGAGAGACGCAGG AGAAGCGCTTCAAGGACCCGGGGCTGGTGGACCAGCTGGTGAAAGCAGACAGTGAGTGGCGACGATGCAG ATTTCGGGCAGACAACTTGAACAAGCTGAAGAATTTATGCAGCAAAACTATTGGGGAGAAAATGAAGAAA AAGGAAGCAGTGGGAGACGACGAGTCCGTCCCAGAGAACGTGCTGAATTTCGATGACCTCACTGCAGACG CGCTAGCTGCCCTGAAAGTCTCACAGATTAAAAAAGTCCGACTCCTCATTGATGAAGCCATCCAGAAGTG TGATGGGGAGCGGGTAAAGCTGGAAGCAGAGCGATTTGAGAACCTCCGCGAGATTGGGAACCTTCTGCAC CCCTCTGTGCCCATTAGTAATGATGAGGACGCAGACAACAAAGTAGAACGTATTTGGGGAGATTGTACAG TCAGGAAGAAGTATTCCCATGTGGACCTGGTGGTGATGGTAGATGGCTTTGAAGGCGAAAAGGGAGCCGT GGTGGCTGGTAGTCGGGGGTACTTCCTGAAGGGGCCCCTGGTGTTCCTGGAGCAGGCGCTTATCCAATAT GCACTGCGTACCTTGGGGAGTCGGGGCTACACTCCAATCTACACCCCCTTCTTCATGAGGAAAGAGGTCA TGCAGGAAGTGGCCCAGCTCAGCCAGTTTGATGAAGAACTTTATAAGGTGATTGGCAAAGGCAGCGAAAA GTCAGATGACAACTCCTATGACGAGAAATACTTGATTGCCACCTCAGAGCAGCCCATCGCGGCTCTGCAC CGGGACGAGTGGCTGCGGCCAGAGGATCTGCCCATCAAGTACGCTGGCCTCTCCACCTGCTTTCGTCAGG AAGTGGGCTCGCATGGCCGTGACACCCGTGGTATCTTCCGAGTCCATCAGTTTGAGAAGATTGAGCAGTT TGTGTACTCATCGCCCCATGACAATAAGTCGTGGGAGATGTTTGATGAGATGATCGCCACCGCAGAAGAA TTCTACCAGTCTTTGGGGATCCCTTACCACATTGTGAATATTGTCTCAGGCTCCTTGAATCACGCTGCCA GTAAGAAGCTCGACCTGGAGGCCTGGTTCCCAGGCTCGGGTGCCTTCCGTGAGTTGGTGTCCTGTTCTAA TTGCACGGATTACCAAGCTCGCCGCCTGAGAATCCGATATGGGCAGACCAAGAAGATGATGGACAAGGTG GAGTTTGTCCATATGCTTAATGCTACAATGTGTGCTACCACCCGGACCATCTGCGCCATCCTGGAGAACT ACCAGGCAGAGAAGGGCATCGCTGTGCCAGAGAAGTTGAGGGAGTTCATGCCGCCAGGGCTCCAAGAGCT GATCCCGTTTGTGAAGCCTGCACCCATTGACCAGGAGCCATCTAAGAAGCAGAAGAAGCAACATGAAGGC AGCAAAAAGAAAGCGAAAGAGGTCCCCCTGGAGAACCAGCTGCAGAGCATGGAGGTCACTGAGGCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001204979
Insert Size	1539 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001204979.1, NP_001191908.1
RefSeq Size	3632 bp
RefSeq ORF	1539 bp
Locus ID	20226
UniProt ID	P26638
Gene Summary	Catalyzes the attachment of serine to tRNA(Ser) in a two-step reaction: serine is first activated by ATP to form Ser-AMP and then transferred to the acceptor end of tRNA(Ser). Is probably also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec). In the nucleus, binds to the VEGFA core promoter and prevents MYC binding and transcriptional activation by MYC. Recruits SIRT2 to the VEGFA promoter, promoting deacetylation of histone H4 at 'Lys-16' (H4K16). Thereby, inhibits the production of VEGFA and sprouting angiogenesis mediated by VEGFA.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.

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