Treh (NM_001277851) Mouse Untagged Clone

CAT#: MC228106

Treh (untagged) - Mouse trehalase (brush-border membrane glycoprotein) (Treh), transcript variant 3



  "NM_001277851" in other vectors (1)

CNY 4,940.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 2210412M19Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC228106 representing NM_001277851
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAGCTCCTGCACCAAGTTCAGATGGCCCAGCTCTACCAAGATGACAAGCAGTTTGTGGATATGTC
ACTGGCCACATCTCCAGATGAAGTCCTGCAGAAGTTCAGTGAGCTGGCCACAGTCCACAACCACAGCATC
CCCAAGGAACAGCTTCAGGAATTTGTCCAGAGTCACTTCCAGCCCGTGGGGCAGGAGCTGCAGTCCTGGA
CCCCTGAGGACTGGAAGGACAGCCCTCAGTTCCTGCAGAAGATCTCGGATGCTAATCTGCGTGTCTGGGC
GGAGGAGCTACACAAGATCTGGAAAAAGCTGGGAAAGAAGGGACTCGTACTGGGTGATGGAAGGCCTGCT
TCTTTCTGAGATGGCCTCAACAGTGAAGGGTATGCTGCAAAACTTTCTGGATCTGGTGAAGACCTACGGA
CATATCCCCAACGGTGGACGCATATATTACCTGCAACGGAGCCAGCCCCCACTCCTGACTCTCATGATGG
ATCGATATGTAGCTCATACCAAGGATGTCGCCTTCCTTCAGGAGAATATTGGGACTCTAGCCTCTGAACT
GGACTTCTGGACTGTGAACAGGACTGTCTCTGTAGTCTCAGGAGGACAAAGCTATGTCTTAAATCGCTAC
TATGTCCCTTATGGGGGACCCAGGCCAGAGTCCTACAGGAAAGACGCAGAATTGGCAAACTCTGTGCCAG
AAGGGGACCGAGAGACTCTGTGGGCTGAGCTCAAGGCTGGGGCTGAGTCTGGCTGGGACTTCTCTTCACG
CTGGCTTGTTGGAGGCCCAGACCCTGATTTGCTCAGCAGCATCCGAACCAGCAAAATGGTACCCGCTGAT
CTGAACGCGTTCCTGTGCCAAGCAGAGGAACTGATGAGTAACTTCTACTCCAGACTAGGGAACGACACAG
AGGCCACAAAGTACAGGAACCTGCGGGCCCAGCGCTTGGCCGCCATGGAAGCTGTCCTGTGGGACGAGCA
GAAGGGTGCCTGGTTTGACTATGACTTGGAAAAGGGGAAGAAGAACCTGGAGTTTTATCCCTCCAACCTC
TCCCCACTTTGGGCTGGCTGCTTCTCAGACCCTAGTGTTGCTGACAAGGCTCTGAAGTACTTGGAGGACA
GCAAGATCTTGACCTACCAATATGGAATCCCAACCTCTCTTCGTAACACAGGCCAGCAGTGGGACTTCCC
CAATGCCTGGGCCCCACTGCAGGACCTGGTCATTAGAGGTTTGGCCAAGTCAGCTTCCCCCCGGACTCAG
GAGGTGGCTTTCCAGCTGGCCCAGAATTGGATCAAAACCAACTTCAAAGTCTACTCCCAAAAGTCAGCGA
TGTTTGAGAAGTATGACATCAGCAACGGTGGACATCCAGGTGGAGGAGGGGAGTATGAAGTTCAGGAAGG
ATTTGGCTGGACAAACGGATTGGCCCTGATGCTTCTGGATCGCTATGGTGACCAGTTGACTTCAGGGACC
CAGTTAGCTTCCCTGGGACCCCACTGCCTAGTGGCTGCCCTTCTTCTCAGTCTTCTGCTACAGTGA


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-RsrII     
ACCN NM_001277851
Insert Size 1536 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001277851.1, NP_001264780.1
RefSeq Size 1952 bp
RefSeq ORF 1536 bp
Locus ID 58866
Gene Summary This gene belongs to the alpha-glucosidase family, whose members encode enzymes that carry out hydrolysis of alpha-glucoside bonds of a variety of carbohydrates. The enzyme encoded by this gene uses the disaccharide trehalose as a highly specific substrate and converts it into two glucose molecules. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2013]
Transcript Variant: This variant (3) differs in the 5' UTR, initiates translation at an alternate start codon and lacks an internal exon, compared to variant 1. The encoded isoform (3) has a distinct N-terminus and is shorter than isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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