Traf2 (NM_001290413) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI325259
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228093 representing NM_001290413 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCAGCCAGTGTGACTTCCCCTGGCTCCCTAGAACTGCTACAGCCTGGCTTCTCCAAGACCCTCC TGGGGACCAGGTTAGAAGCCAAGTACCTCTGTTCAGCCTGCAAAAACATCCTGCGGAGGCCTTTCCAGGC CCAGTGTGGGCACCGCTACTGCTCCTTCTGCCTGACCAGCATCCTCAGGTGTGCATCCATTCTCTCCAGC TCTGGGCCCCAGAACTGTGCTGCCTGTGTCTATGAAGGCCTGTATGAAGAAGGCATTTCTATTTTAGAGA GTAGTTCGGCCTTTCCAGATAACGCTGCCCGCAGAGAGGTGGAGAGCCTGCCAGCTGTCTGTCCCAATGA TGGATGCACTTGGAAGGGGACCTTGAAAGAATACGAGAGCTGCCACGAAGGACTTTGCCCATTCCTGCTG ACGGAGTGTCCTGCATGTAAAGGCCTGGTCCGCCTCAGCGAGAAGGAGCACCACACTGAGCAGGAATGCC CCAAAAGGAGCCTGAGCTGCCAGCACTGCAGAGCACCCTGTAGCCACGTGGACCTGGAGGTACACTATGA GGTCTGCCCCAAGTTTCCCTTAACCTGTGATGGCTGTGGCAAGAAGAAGATCCCTCGGGAGACGTTTCAG GACCATGTTAGAGCATGCAGCAAATGCCGGGTTCTCTGCAGATTCCACACCGTTGGCTGTTCAGAGATGG TGGAGACTGAGAACCTGCAGGATCATGAGCTGCAGCGGCTACGGGAACACCTAGCCCTACTGCTGAGCTC ATTCTTGGAGGCCCAAGCCTCTCCAGGAACCTTGAACCAGGTGGGGCCAGAGCTACTCCAGCGGTGCCAG ATTTTGGAGCAGAAGATAGCAACCTTTGAGAACATTGTCTGCGTCTTGAACCGTGAAGTAGAGAGGGTAG CAGTGACTGCAGAGGCTTGTAGCCGGCAGCACCGGCTAGACCAGGACAAGATTGAGGCCCTGAGTAACAA GGTGCAACAGCTGGAGAGGAGCATCGGCCTCAAGGACCTGGCCATGGCTGACCTGGAGCAGAAGGTCTCC GAGTTGGAAGTATCCACCTATGATGGGGTCTTCATCTGGAAGATCTCTGACTTCACCAGAAAGCGTCAGG AAGCCGTAGCTGGCCGGACACCAGCTATCTTCTCCCCAGCCTTCTACACAAGCAGATATGGCTACAAGAT GTGTCTACGAGTCTACTTGAATGGCGACGGCACTGGGCGGGGAACTCATCTGTCTCTCTTCTTCGTGGTG ATGAAAGGCCCCAATGATGCTCTGTTGCAGTGGCCTTTTAATCAGAAGGTAACATTGATGTTGCTGGACC ATAACAACCGGGAGCATGTGATCGACGCATTCAGGCCCGATGTAACCTCGTCCTCCTTCCAGAGGCCTGT CAGTGACATGAACATCGCCAGTGGCTGCCCCCTCTTCTGCCCTGTGTCCAAGATGGAGGCCAAGAATTCC TATGTGCGGGATGATGCGATCTTCATCAAAGCTATTGTGGACCTAACAGGACTCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290413
Insert Size	1527 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290413.1, NP_001277342.1
RefSeq Size	3065 bp
RefSeq ORF	1527 bp
Locus ID	22030
UniProt ID	P39429
Gene Summary	Regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis. Required for normal antibody isotype switching from IgM to IgG. Has E3 ubiquitin-protein ligase activity and promotes 'Lys-63'-linked ubiquitination of target proteins, such as BIRC3, RIPK1 and TICAM1. Is an essential constituent of several E3 ubiquitin-protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases. Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation; this does not depend on the TRAF2 RING-type zinc finger domain. Isoform 2 does not seem to mediate activation of NF-kappa-B but inhibits isoform 1 activity. Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR. In complex with BIRC2 or BIRC3, promotes ubiquitination of IKBKE.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).

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