Tcp1 (NM_001290712) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI528772; c-cpn; CCT; Cct1; Ccta; p63; Tcp-1; Tp63; TRic
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228088 representing NM_001290712 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGTAACCATTACTAACGATGGTGCCACCATCCTGAAGTTACTGGAGGTAGAACATCCCGCAGCCA AAGTTCTGTGTGAGCTGGCTGACCTGCAAGACAAAGAAGTTGGAGATGGAACTACCTCAGTGGTAATCAT TGCAGCGGAGCTTCTGAAAAATGCAGATGAGCTAGTCAAACAGAAAATTCATCCAACATCAGTTATTAGT GGCTATCGTCTTGCCTGCAAGGAAGCGGTGCGTTATATCAATGAGAACCTGATTATTAACACAGACGAAC TTGGAAGAGACTGTCTGATCAATGCTGCTAAGACATCCATGTCATCCAAAATTATTGGAATAAATGGTGA TTACTTTGCTAATATGGTAGTAGATGCTGTGCTTGCTGTTAAATACACAGATGCCAGAGGCCAGCCTCGC TATCCAGTCAATTCTGTTAATATTCTGAAAGCCCATGGGAGAAGTCAGATAGAAAGCATGCTGATCAATG GCTATGCGCTCAATTGTGTGGTTGGATCTCAGGGCATGCCCAAGAGAATAGTTAATGCAAAAATTGCTTG TCTTGACTTCAGCCTGCAGAAAACAAAAATGAAGCTTGGTGTACAGGTGGTTATTACAGACCCTGAGAAA TTGGACCAAATTAGACAGAGAGAATCGGATATCACCAAGGAGAGAATTCAGAAGATCCTGGCAACTGGTG CCAATGTTATTCTAACCACTGGTGGCATTGATGATATGTATCTCAAGTATTTTGTGGAAGCTGGTGCCAT GGCTGTTAGGAGAGTTTTAAAACGAGACCTGAAGCATGTTGCAAAAGCTTCTGGAGCAAGTATCCTGTCT ACGCTGGCCAATTTGGAAGGCGAAGAAACTTTTGAAGTGACGATGTTGGGACAAGCGGAAGAGGTCGTAC AGGAGAGAATTTGTGATGATGAGCTGATCTTAATCAAAAATACTAAGGCTCGTACATCTGCTTCAATCAT CTTACGAGGAGCAAATGATTTCATGTGTGATGAAATGGAGCGCTCTTTACATGATGCTCTTTGTGTGGTG AAGAGAGTTTTGGAGTTGAAATCTGTGGTCCCAGGTGGAGGTGCTGTAGAAGCTGCCCTGTCCATATACC TGGAAAACTATGCAACAAGCATGGGATCTCGGGAACAGCTTGCTATTGCAGAGTTTGCAAGATCTCTGCT TGTGATTCCTAATACACTGGCAGTGAATGCTGCCCAGGACTCCACCGACCTGGTTGCCAAGTTAAGAGCT TTTCACAATGAGGCTCAAGTGAACCCGGAACGTAAAAATCTAAAGTGGATTGGTCTTGATTTGGTCCATG GGAAACCACGAGACAACAAGCAAGCAGGGGTGTTTGAACCAACCATAGTTAAAGTGAAGAGCCTGAAGTT CGCAACAGAGGCTGCAATCACCATCCTTCGGATTGATGATCTGATAAAATTACACCCAGAAAGCAAAGAC GATAAACACGGAAGTTATGAAAATGCTGTTCACTCTGGAGCCCTTGATGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290712
Insert Size	1524 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290712.1, NP_001277641.1
RefSeq Size	2413 bp
RefSeq ORF	1524 bp
Locus ID	21454
UniProt ID	P11983
Gene Summary	Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. The TRiC complex plays a role in the folding of actin and tubulin.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate exon in the 5' coding region and initiates translation at an alternate start codon compared to variant 1. The encoded protein (isoform 2) has a distinct N-terminus and is shorter than isoform 1.

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