Sytl4 (NM_001290718) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Gph; Slp4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228064 representing NM_001290718 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGAGATACTAGACCTCTCTTTTCTGTCCGAGATGGAAAGGGATTTGATCCTCGGTGTTCTGCAGA GAGATGAAGAACTCAGAAAAGCAGATGAGAAAAGGATTCGGCGACTGAAGAATGAGCTCCTGGAGATCAA AAGAAAAGGGGCCAAAAGGGGCAGCCAACACTATAGTGATCGAACCTGTGCCAGGTGCCAGGAGGGCCTG GGCCGCTTAATTCCCAAAAGCAGCACTTGTGTGGGCTGCAATCATTTAGTGTGCCGAGAATGCCGAGTTC TGGAAAGCAATGGCTCTTGGAGGTGCAAGGTGTGCTCGAAGGAGATAGAGCTGAAGAAAGCAACTGGAGA TTGGTTTTATGACCAGAAAGTAAATCGCTTTGATTACCGAACAGGCAGCGAGATAATCAGGATGTCCCTG CGCCAGAAGCCTGCAGTGAATAAAAGAGAGACAGCGGGGCAGTCCCTCCTTCAGCAGACACAGATGGGTG ATATCTGGCCAGGAAGAAGGATCATTCAAGAACAACAACAGAGGGAACAAAGTGTGCTGTTTGAAGTGCC AAAGACCAGAAGTGGAAAGAGTGCACTGGAGGCTGAGAGCGAGAGTCTGGATAGCTACACTGCTGACTCA GACAGCACCTCCAGGAGAGATTCTCTGGATAAATCTGGACTCTTCCCAGAATGGAAGAAGATGTCCGCTC CCAAATCTCAAGTTGAAAAGGAAATTCCACCAGGGAACCAAAATGCAGTCTGTGGTGATGAGGGTGACAT GGTATTCAAGAAGAACACCAAAAAAGTCCTGAGGCCTTCAGAATACACTAAGTCTGTGATAGACCTTCGC CCAGAAGATGTGGCCCAAGAAAGTGGCATCTTGGGAGACAGAAGCAAATCTGTACCAGGCCTCAGTGTGG ATATGGAAGAAGAGGAGGAGGAGGAAGAGGACATTGACCACCTGGTGAAGTTGCACCGCCAGAAACTGGC CAGAGGCAGCATGCAGAGTGGCTCCTCCATGAGTACCCTTGGCAGCATTATGAGTATCTATAGCGAAGCT GGTGATTTTGGGAACATCTCTGTGACGGGCAAGATTGCCTTTTCACTGAAGTTTGAGCAGAAAACACAGA CTTTGGTCATCCATGTCAAGGAGTGCCACCAGCTGGCCTATGCTGATGAAGCCAAGAAGCGTTCTAACCC ATATGTGAAGACTTATCTTCTGCCTGACAAGTCCCGCCAAGGAAAAAGAAAAACCAGCATCAAGCGGGAC ACCATCAATCCACTATATGATGAGACCTTTCGGTACGAGATTTCGGAATCTCTTCTGGCTCAGAGGACTT TGCAGTTTTCCGTTTGGCATCATGGTCGTTTTGGCAGAAACACTTTCCTTGGAGAGGCGGAGGTCCACAT GGACTCTTGGAAGCTGGATAAGAAGCTGGATCACTGCCTCCCTTTACATGGAAAGGGATCAGTAATGGCG AAGTGGTGGACTGGATGGATTCGACTGGTGAAGAAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290718
Insert Size	1509 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290718.1, NP_001277647.1
RefSeq Size	3325 bp
RefSeq ORF	1509 bp
Locus ID	27359
UniProt ID	Q9R0Q1
Gene Summary	Modulates exocytosis of dense-core granules and secretion of hormones in the pancreas and the pituitary. Interacts with vesicles containing negatively charged phospholipids in a Ca(2+)-independent manner.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) uses an alternate splice site in the 5' UTR and lacks three alternate exons, which results in a frameshift, compared to variant 1. The encoded isoform (2) has a distinct C-terminus and is shorter than isoform 1. Variants 2 and 3 encode the same isoform (2).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.