Cacnb4 (NM_001285426) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	3110038O15Rik; Cchb4; lh
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228062 representing NM_001285426 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGTGGTGGCCCGAGGCACCACGACCCGGAGGAGCAGGTTGAAAAGATCGGATGGCAGCACCACTT CTACCAGCTTCATCCTCAGACAGGGGTCAGCAGATTCCTATACAAGCAGGCCATCGGACTCCGATGTCTC CTTGGAAGAGGACCGGGAAGCGATCCGGCAAGAGCGAGAACAGCAAGCAGCTATCCAGCTTGAGAGAGCG AAGTCCAAACCTGTAGCTTTTGCGGTGAAAACGAACGTGAGCTACTGTGGTGCCCTGGACGAAGATGTGC CCGTTCCCAGCACAGCCATCTCCTTTGACGCCAAGGACTTTCTTCACATTAAAGAGAAATATAACAATGA TTGGTGGATAGGAAGACTGGTAAAAGAGGGCTGTGAGATTGGCTTCATCCCAAGTCCACTGCGCTTGGAG AATATTCGGATTCAACAGGAACAGAAAAGAGGCCGTTTTCATGGCGGGAAATCGAGTGGAAACTCTTCCT CCAGTCTGGGAGAAATGGTATCAGGAACATTCCGAGCAACTCCCACAACAACAGCAAAACAGAAGCAGAA AGTGACGGAGCACATTCCTCCGTATGACGTCGTGCCGTCAATGCGTCCTGTGGTGTTAGTGGGGCCATCA CTGAAAGGTTATGAGGTAACAGACATGATGCAGAAAGCCCTCTTTGATTTCCTGAAGCACAGGTTTGATG GGAGGATATCAATAACAAGAGTGACAGCTGACATTTCTCTTGCTAAGAGATCTGTCCTCAACAATCCTAG CAAGAGAGCAATAATTGAACGTTCCAACACCAGATCCAGCTTAGCGGAAGTACAAAGTGAAATTGAAAGA ATTTTTGAGTTGGCAAGATCTTTGCAATTGGTTGTTCTTGATGCAGACACCATCAACCACCCAGCACAGC TGATAAAGACATCCTTAGCACCCATCATCGTCCACGTGAAGGTCTCGTCCCCAAAGGTTTTACAGCGGCT GATTAAGTCCAGAGGAAAGTCCCAAAGCAAACACTTGAATGTTCAACTGGTGGCGGCCGATAAACTGGCC CAGTGCCCGCCTGAAATGTTTGATGTTATATTAGATGAGAATCAACTTGAGGATGCCTGTGAACATCTGG GAGAGTACCTGGAGGCATACTGGCGTGCCACCCACACGAGCAGTAGCACCCCTATGACCCCATTACTGGG GCGGAACGTGGGCTCCACAGCCCTCTCACCATATCCCACAGCAATCTCTGGATTACAGAGTCAGCGAATG AGACACAGCAACCATTCTACAGAGAATTCTCCAATTGAAAGACGAAGCCTAATGACCTCGGATGAAAATT ACCACAATGAGAGGGCCCGCAAGAGTAGGAACCGCTTGTCTTCCAGCTCCCAGCACAGCCGAGACCACTA CCCTCTGGTGGAAGAAGATTACCCGGACTCGTACCAGGACACTTATAAGCCCCATAGGAACCGAGGATCG CCCGGGGGGTGCAGCCATGACTCCCGACATAGGCTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001285426
Insert Size	1509 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001285426.1, NP_001272355.1
RefSeq Size	7792 bp
RefSeq ORF	1509 bp
Locus ID	12298
Gene Summary	The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR and CDS, and uses a downstream start codon compared to variant 1. The resulting protein (isoform c) has a shorter N-terminus compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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